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Effects of dissolved oxygen on phosphorus transformation in reservoir sediments: novel insights on bacterial community and functional genes

  • Sediments, Sec 2 • Physical and Biogeochemical Processes • Research Article
  • Published:
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Abstract

Purpose

Dissolved oxygen (DO) has important effects on microorganisms, phosphorus cycle genes, and phosphorus release from sediments. However, the mechanism by which DO affects the release of phosphorus through microorganisms and genes is not obvious. This paper aims to investigate how microorganisms and genes affect the release of phosphorus from sediments under different dissolved oxygen conditions.

Materials and methods

Sediment and water samples were collected and placed in incubators for 30 and 60 days of culture. Dissolved oxygen concentrations were maintained at 0.5 mg L−1, 1 mg L−1, 2 mg L−1, and 3 mg L−1. Fluorescence was used to quantitatively determine the number of functional genes, obtain microbial species through high-throughput sequencing, and analyze the correlation with phosphorus in sediments.

Results and discussion

At the genus level, Pseudomonas has a greater contribution to mineralization. On the 30th day, as the concentration of DO increased, the number of functional genes (phoD, ppk, and qppC) increased significantly. The expression of phoD (alkaline phosphatase gene) and pqqC (pyrroloquinoline quinone) together promoted the release of phosphorus. After 60 days culture, the ReP (the release of phosphorus from sediments) content decreased from 53.5 to 14.2 mg kg−1 with increasing DO concentration.

Conclusions

OrP (organic phosphorus) and AcP (acid extracted phosphorus) are converted to AlP (alkali extracted phosphorus) and thus lead to the reduction of ReP. The expression of ppk (polyphosphate kinase) gene was inhibited under low DO and promoted the release of phosphorus.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. U20A20316, No. 51509199, and No. 72091510) and the Natural Science Foundation of Hebei Province (Grant No. E2020402074 and No. E2020402044).

Author information

Authors and Affiliations

  1. Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing, 100124, China

    Yi Ding & Hao Wang

  2. Hebei Key Laboratory of Intelligent Water Conservancy, School of Water Conservancy and Hydroelectric, Hebei University of Engineering, Handan, 056038, China

    Qi Zhang & Beibei Chai

  3. Hebei Collaborative Innovation Center for the Regulation and Comprehensive Management of Water Resources and Water Environment, Hebei University of Engineering, Handan, 056038, China

    Beibei Chai

  4. State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research, Beijing, 100038, China

    Xiaohui Lei & Mao Ye

  5. State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Beijing Normal University, Beijing, 100875, China

    Bin Chen

Authors
  1. Yi Ding
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  2. Hao Wang
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  3. Qi Zhang
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  4. Beibei Chai
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  5. Xiaohui Lei
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  6. Mao Ye
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  7. Bin Chen
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Corresponding authors

Correspondence to Beibei Chai or Xiaohui Lei.

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The authors declare no competing interests.

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Responsible editor: Haihan Zhang

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Highlights

• Our work provides insights into the role of bacteria in phosphorus release from sediments at various levels of dissolved oxygen.

• The relative abundance of the four most abundant genera containing ppk, including Pseudomonas, Variovorax, Rhodococcus, and Luteitalea, was positively related to the content of OrP, but the relative abundance of Pseudomonas was negatively related to OrP content.

• Pseudomonas had a greater contribution in mineralization of OrP, but Variovorax, Rhodococcus, and Luteitalea mainly promoted OrP synthesis.

• Some genera containing pqqC, including Candidatus Solibacter, Luteitalea, Gemmatirosa, Pseudomonas, and Granulicella, may produce organic acids that do not bind phosphate to calcium.

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Ding, Y., Wang, H., Zhang, Q. et al. Effects of dissolved oxygen on phosphorus transformation in reservoir sediments: novel insights on bacterial community and functional genes. J Soils Sediments 22, 2094–2104 (2022). https://doi.org/10.1007/s11368-022-03233-9

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  • DOI: https://doi.org/10.1007/s11368-022-03233-9

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