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Biogeographical distribution of dissimilatory nitrate reduction to ammonium (DNRA) bacteria in wetland ecosystems around the world

Journal of Soils and Sediments volume 20, pages 3769–3778 (2020)Cite this article

Abstract

Purpose

Nitrate (NO3−) is the second preferred terminal electron acceptor after oxygen (O2), as it plays an essential role as the main electron acceptor for respiration under oxygen-depleted conditions. Dissimilatory nitrate reduction to ammonium (DNRA) is an increasingly important process of microbial-driven nitrate reduction in wetland ecosystems, making it necessary to study the biogeographical distribution and structure of the DNRA community, and to identify the factors governing DNRA in global wetlands.

Materials and methods

We collected samples from 15 wetland sites around the world and used qPCR assay analysis to quantify the DNRA functional gene nrfA. High-throughput sequencing was conducted to analyze the microbial diversity and community structure of DNRA bacteria. The most connected genera were derived from molecular ecological network analysis. Principal coordinates analysis (PCoA), redundancy analysis (RDA), and Pearson’s correlation analysis were used to explore the relationship between microbial structure and environmental factors.

Results and discussion

The environmental conditions of the wetlands varied largely with the latitude. At the phylum level, Proteobacteria was dominant, and Anaerolinea was the key genus. Pearson’s correlation analysis also illustrated that the annual average temperature, as a factor of latitude, most significantly affected DNRA abundance, followed by total organic matter (TOM) and C/N ratio.

Conclusions

For the first time, we summarized the characteristics of DNRA bacteria at the molecular level, along with the influencing factors, in wetlands with a wide biogeographical distribution. This study provides a scientific foundation for the future study of DNRA bacteria in wetlands around the world.

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Acknowledgments

The author Guibing Zhu gratefully acknowledges the support of a Humboldt Research Fellowship (1152633) and Program of the Youth Innovation Promotion Association (CAS).

Funding

This research is financially supported by the National Natural Science Foundation of China (No.41671471, 41322012, and 21707155), China Postdoctoral Science Foundation (2017M620931), Strategic Priority Research Program of the Chinese Academy of Sciences (XDB15020303), National Key R&D Program (2016YFA0602303), Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (2017BT01Z176), and special fund from the State Key Joint Laboratory of Environment Simulation and Pollution Control.

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Authors and Affiliations

  1. Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

    Huawei Pan, Dongdan Yuan, Weiyue Liu, Yanxia Pi, Shanyun Wang & Guibing Zhu

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Weiyue Liu & Guibing Zhu

Authors
  1. Huawei Pan
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  2. Dongdan Yuan
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  3. Weiyue Liu
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  4. Yanxia Pi
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  5. Shanyun Wang
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Correspondence to Guibing Zhu.

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Pan, H., Yuan, D., Liu, W. et al. Biogeographical distribution of dissimilatory nitrate reduction to ammonium (DNRA) bacteria in wetland ecosystems around the world. J Soils Sediments 20, 3769–3778 (2020). https://doi.org/10.1007/s11368-020-02707-y

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Keywords

  • Abundance
  • Biogeographical distribution
  • DNRA
  • High-throughput sequencing
  • Wetland ecosystems