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Effects of electron donors on the degradation of hexachlorocyclohexane and microbial community in submerged soils

  • Soils, Sec 4 • Ecotoxicology • Research Article
  • Published:
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Abstract

Purpose

Hexachlorocyclohexane (HCH) is a pesticide that is persistent in aerobic but not in anaerobic conditions. The aim of this investigation was to elucidate the impacts of different electron donors on the degradation of hexachlorocyclohexane and their relationships with the microbial community in submerged soils.

Materials and methods

Soil samples were collected from a pesticides-polluted site which was previously used for long-term storage of pesticides since the 1960s. Residual amounts of HCHs in submerged soils were assessed to evaluate the efficiency of the added electron donors after incubation. Changes in microbial community were monitored during incubation.

Results and discussion

The efficiencies of removal of HCHs by Fe0, Fe0 + straw and glucose treatments were 63.8%, 40.3%, and 22.0%, compared with 5.4% by the control. Soil redox potential decreased rapidly following addition of Fe0 and became anaerobic, but there was no significant decrease in redox potential following the addition of glucose. Principal coordinates analysis (PCoA) confirms that the electron donors greatly affected the soil bacterial community. Furthermore, community composition analysis shows that Firmicutes was the dominant phylum after addition of carbon sources (straw and glucose) but Proteobacteria were dominant in the control and the Fe0 treatment. Prauserella, Actinobacteria, and Streptomyces showed highly significant increases in the Fe0 treatment and were positively correlated with total HCH residues.

Conclusions

These findings indicate that the addition of organic materials (straw and glucose) had a gradual effect on the degradation of HCH while Fe0 acted rapidly. Elucidation of the relationships between electron donors and soil microbial communities will help in the bioremediation of HCH-contaminated sites.

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References

  • Adrian L, Löffler FE (2016) Organohalide-respiring bacteria. Springer-Verlag, Berlin Heidelberg

    Book  Google Scholar 

  • Alvarez A, Benimeli CS, Saez JM, Fuentes MS, Cuozzo SA, Polti MA, Amoroso MJ (2012) Bacterial bio-resources for remediation of hexachlorocyclohexane. Int J Mol Sci 13:15086–15106

    Article  CAS  Google Scholar 

  • Ammeri RW, Mehri I, Badi S, Hassen W, Hassen A (2017) Pentachlorophenol degradation by Pseudomonas fluorescens. Water Qual Res J Can 52:99–108

    Article  CAS  Google Scholar 

  • Anh TPN et al (2011) Characterization of the 1,1-dichloro-2,2-bis(4-chlorophenylln ethylene (DDE)) degradation system in janibacter sp TYM3221. Enzym Microb Technol 49:532–539

    Article  Google Scholar 

  • Badea SL, Vogt C, Weber S, Danet AF, Richnow HH (2009) Stable isotope fractionation of gamma-hexachlorocyclohexane (lindane) during reductive dechlorination by two strains of sulfate-reducing bacteria. Environ Sci Technol 43:3155–3161

    Article  CAS  Google Scholar 

  • Benimeli CS, Castro GR, Chaile AP, Amoroso MJ (2006) Lindane removal induction by Streptomyces sp M7. J Basic Microbiol 46:348–357

    Article  CAS  Google Scholar 

  • Benimeli CS, Castro GR, Chaile AP, Amoroso MJ (2007) Lindane uptake and degradation by aquatic Streptomyces sp strain M7. Int Biodeterior Biodegrad 59:148–155

    Article  CAS  Google Scholar 

  • Chen M, Liu C, Li X, Huang W, Li F (2014) Iron reduction coupled to reductive dechlorination in red soil. Soil Sci 179:457–467

    Article  CAS  Google Scholar 

  • Chen M, Tong H, Li F, Liu C, Lan Q, Liu C (2018) The effect of electron donors on the dechlorination of pentachlorophenol (PCP) and prokaryotic diversity in paddy soil. Eur J Soil Biol 86:8–15

    Article  CAS  Google Scholar 

  • Cong X, Xue N, Wang S, Li K, Li F (2010) Reductive dechlorination of organochlorine pesticides in soils from an abandoned manufacturing facility by zero-valent iron. Sci Total Environ 408:3418–3423

    Article  CAS  Google Scholar 

  • Cong X, Li FS, Kelly RM, Xue ND (2018) Distribution and removal of organochlorine pesticides in waste clay bricks from an abandoned manufacturing plant using low-temperature thermal desorption technology. Environ Sci Pollut Res 25:12119–12126

    Article  CAS  Google Scholar 

  • Cruz-Gonzalez G et al (2018) Degradation of chlordecone and beta-hexachlorocyclohexane by photolysis, (photo-)Fenton oxidation and ozonation. J Environ Sci Health Part B Pestic Contam Agric Wastes 53:121–125

    Article  CAS  Google Scholar 

  • Cui ZJ, Meng FL, Hong JL, Li XZ, Ren XH (2012) Effects of electron donors on the microbial reductive dechlorination of hexachlorocyclohexane and on the environment. J Biosci Bioeng 113:765–770

    Article  CAS  Google Scholar 

  • Dominguez CM, Oturan N, Romero A, Santos A, Oturan MA (2018) Removal of lindane wastes by advanced electrochemical oxidation. Chemosphere 202:400–409

    Article  CAS  Google Scholar 

  • Elcey CD, Kunhi AAM (2010) Substantially enhanced degradation of hexachlorocyclohexane isomers by a microbial consortium on acclimation. J Agric Food Chem 58:1046–1054

    Article  CAS  Google Scholar 

  • Fan YT, Zhang YH, Zhang SF, Hou HW, Ren BZ (2006) Efficient conversion of wheat straw wastes into biohydrogen gas by cow dung compost. Bioresour Technol 97:500–505

    Article  CAS  Google Scholar 

  • Gao YF, Yang H, Zhan XH, Zhou LX (2013) Scavenging of BHCs and DDTs from soil by thermal desorption and solvent washing. Environ Sci Pollut Res 20:1482–1492

    Article  CAS  Google Scholar 

  • Huang Y, Pan H, Wang Q, Ge Y, Liu W, Christie P (2019) Enrichment of the soil microbial community in the bioremediation of a petroleum-contaminated soil amended with rice straw or sawdust. Chemosphere 224:265–271

    Article  CAS  Google Scholar 

  • Lal R, Pandey G, Sharma P, Kumari K, Malhotra S, Pandey R, Raina V, Kohler HP, Holliger C, Jackson C, Oakeshott JG (2010) Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation. Microbiol Mol Biol Rev 74:58–80

    Article  CAS  Google Scholar 

  • Lane DJ (1991) 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics, pp 115–175

  • Langenhoff AAM, Staps SJM, Pijls C, Rijnaarts HHM (2013) Stimulation of hexachlorocyclohexane (HCH) biodegradation in a full scale in situ bioscreen. Environ Sci Technol 47:11182–11188

    Article  CAS  Google Scholar 

  • Li YF (1999) Global technical hexachlorocyclohexane usage and its contamination consequences in the environment: from 1948 to 1997. Sci Total Environ 232:121–158

    Article  CAS  Google Scholar 

  • MacRae IC, Yamaya Y, Yoshida T (1984) Persistence of hexachlorocyclohexane isomers in soil suspensions soil. Biol Biochem 16:285–286

    Article  CAS  Google Scholar 

  • Mansouri A, Cregut M, Abbes C, Durand MJ, Landoulsi A, Thouand G (2017) The environmental issues of DDT pollution and bioremediation: a multidisciplinary review. Appl Biochem Biotechnol 181:309–339

    Article  CAS  Google Scholar 

  • Middeldorp PJM, van Doesburg W, Schraa G, Stams AJM (2005) Reductive dechlorination of hexachlorocyclohexane (HCH) isomers in soil under anaerobic conditions. Biodegradation 16:283–290

    Article  CAS  Google Scholar 

  • Ni MF, Tian SL, Huang QF, Yang YM (2018) Electrokinetic-Fenton remediation of organochlorine pesticides from historically polluted soil. Environ Sci Pollut Res 25:12159–12168

    Article  CAS  Google Scholar 

  • Papale M, Giannarelli S, Francesconi S, di Marco G, Mikkonen A, Conte A, Rizzo C, de Domenico E, Michaud L, Giudice AL (2017) Enrichment, isolation and biodegradation potential of psychrotolerant polychlorinated-biphenyl degrading bacteria from the Kongsfjorden (Svalbard Islands, High Arctic Norway). Mar Pollut Bull 114:849–859

    Article  CAS  Google Scholar 

  • Phillips TM, Lee H, Trevors JT, Seech AG (2006) Full-scale in situ bioremediation of hexachlorocyclohexane-contaminated soil. J Chem Technol Biotechnol 81:289–298

    Article  CAS  Google Scholar 

  • Sadiq S, Mahmood-ul-Hassan M, Ahad K, Nazir S (2018) Bioremediation of hexachlorocyclohexane (HCH) in soil using spent mushroom compost of Pleurotus ostreatus. Bioremediat J 22:126–135

    Article  CAS  Google Scholar 

  • Sahoo B, Ningthoujam R, Chaudhuri S (2019) Isolation and characterization of a lindanedegrading bacterium Paracoccus sp. NITDBR1 and evaluation of its plant growth promoting traits. Int Microbiol 22:155–167

    Article  CAS  Google Scholar 

  • Shoiful A, Ueda Y, Nugroho R, Honda K (2016) Degradation of organochlorine pesticides (OCPs) in water by iron (Fe)-based materials. J Water Process Eng 11:110–117

    Article  Google Scholar 

  • Sineli PE, Tortella G, Davila Costa JS, Benimeli CS, Cuozzo SA (2016) Evidence of alpha-, beta- and gamma-HCH mixture aerobic degradation by the native actinobacteria Streptomyces sp M7. World J Microbiol Biotechnol 32:1–9

    Article  CAS  Google Scholar 

  • Sun GD, du Y, Yin J, Jiang Y, Zhang D, Jiang B, Li G, Wang H, Kong F, Su L, Hu J (2019) Response of microbial communities to different organochlorine pesticides (OCPs) contamination levels in contaminated soils. Chemosphere 215:461–469

    Article  CAS  Google Scholar 

  • Vergani L, Mapelli F, Suman J, Cajthaml T, Uhlik O, Borin S (2019) Novel PCB-degrading Rhodococcus strains able to promote plant growth for assisted rhizoremediation of historically polluted soils. PLoS One 14:e0221253

    Article  CAS  Google Scholar 

  • Wang B et al (2017) Biosurfactant-producing microorganism Pseudomonas sp. SB assists the phytoremediation of DDT-contaminated soil by two grass species. Chemosphere 182:137–142

    Article  CAS  Google Scholar 

  • Xu Y, Niu L, Qiu J, Zhou Y, Lu H, Liu W (2018) Stereoselective accumulations of hexachlorocyclohexanes (HCHs) are correlated with Sphingomonas spp. in agricultural soils across China. Environ Pollut 240:27–33

    Article  CAS  Google Scholar 

  • Yang SC, Lei M, Chen TB, Li XY, Liang Q, Ma C (2010) Application of zerovalent iron (Fe(0)) to enhance degradation of HCHs and DDX in soil from a former organochlorine pesticides manufacturing plant. Chemosphere 79:727–732

    Article  CAS  Google Scholar 

  • Yang CW, Huang HW, Chang BV (2017) Microbial communities associated with anaerobic degradation of polybrominated diphenyl ethers in river sediment. J Microbiol Immunol Infect 50:32–39

    Article  CAS  Google Scholar 

Download references

Funding

We thank the National Natural Science Foundation of China (U1662110, 41877141) and National Science and Technology Major Project (2018YFC1801301-002) for financial support.

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Authors and Affiliations

  1. School of Environmental and Safety Engineering, Changzhou University, Changzhou, 213164, China

    Yuan Zhao & Yun Zhang

  2. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Yun Zhang, Jinyu Hou, Qingling Wang, Wuxing Liu, Yucheng Wu & Peter Christie

  3. Institute of Solid Waste and Soil, Chongqing Research Academy of Eco-Environmental Sciences, Chongqing, 401147, China

    Jun Wang

Authors
  1. Yuan Zhao
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  2. Yun Zhang
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  3. Jun Wang
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  4. Jinyu Hou
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  5. Qingling Wang
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  6. Wuxing Liu
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  7. Yucheng Wu
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  8. Peter Christie
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Correspondence to Wuxing Liu.

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Responsible editor: Dong-Mei Zhou

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Zhao, Y., Zhang, Y., Wang, J. et al. Effects of electron donors on the degradation of hexachlorocyclohexane and microbial community in submerged soils. J Soils Sediments 20, 2155–2165 (2020). https://doi.org/10.1007/s11368-020-02593-4

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  • DOI: https://doi.org/10.1007/s11368-020-02593-4

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