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Effects of Spartina alterniflora invasion on Kandelia candel rhizospheric bacterial community as determined by high-throughput sequencing analysis

Journal of Soils and Sediments volume 19, pages 332–344 (2019)Cite this article

Abstract

Purpose

The biomass and diversity of mangroves suffer a significant decline when S. alterniflora invades in China. The objective of this study was to examine the effects of S. alterniflora invasion on Kandelia candel rhizospheric bacterial community and to explore the control factors of the soil function.

Materials and methods

In this study, the high-throughput sequencing method was applied to assess the composition and diversity of rhizospheric bacterial community in mangrove community (MC), S. alterniflora community (SC), and mangrove-S. alterniflora community (MS). Relationships were also analyzed between the relative abundances of bacterial communities and soil nutrients.

Results and discussion

The results revealed that S. alterniflora invasion resulted in a significant decline of rhizospheric nutrients. The S. alterniflora invades and exacerbated the microecological imbalance in the rhizospheric soils of MS, and markedly decreased the soil bacterial community diversity. The change trends of five alpha diversity indexes followed the order of SC > MC > MS. At the phylum level, S. alterniflora invasion resulted in a significant increase in the relative abundance of Proteobacteria and Euryarchaeota, but a significant decrease of Firmicutes and Nitrospirae. At the genus level, S. alterniflora invasion resulted in obvious differences in rhizospheric bacterial community of MS. Both the correlation and redundancy analyses suggested that the soil nutrient content was the main soil factor affecting the relative abundance of bacterial communities, and the soil nutrients play an important role in the shifts of soil bacterial community diversity.

Conclusions

This study documented the degradation of soil nutrients and the significant variations of rhizospheric bacterial community in MS under S. alterniflora invasion.

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Acknowledgments

The authors would like to thank Wu Linkun, Chen Jun, and Wu Hongmiao in Fujian Agriculture and Forestry University for experimental guidance during the study. We also thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Funding

This work was supported by the Chinese National Natural Science Foundation (31500443), and the Scientific Research Foundation of Fujian Agriculture and Forestry University (XJQ201718) and Fujian-Taiwan Joint Innovative Centre for Germplasm Resources and Cultivation of Crop (grant no. 2015-75; FJ 2011 Program, China).

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Author notes

  1. Jinfu Liu and Zeyan Wu contributed equally to this work

Authors and Affiliations

  1. Life Sciences College, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Jie Zheng, Yiqi Lan & Jinfu Liu

  2. Forestry College, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

    Jie Zheng, Jianjuan Li, Yiqi Lan & Jinfu Liu

  3. Life Sciences College, Yantai University, Yantai Shi, 264000, Shandong, China

    Sidi Liu

  4. Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Liuting Zhou, Yang Luo & Zeyan Wu

  5. Cross-Strait Nature Reserve Research Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Jie Zheng, Jinfu Liu & Zeyan Wu

  6. Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou, 350002, China

    Zeyan Wu

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  1. Jie Zheng
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Corresponding authors

Correspondence to Jinfu Liu or Zeyan Wu.

Ethics declarations

This study has been approved by the Minjiang River Estuary Wetland National Nature Reserve Management Committee, which takes care of the planning and protecting of Minjiang River Estuary Wetland. The study did not involve any endangered or protected species. All the data in this study can be published and shared.

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The authors declare no competing financial interests.

Additional information

Responsible editor: Li Zhang

Electronic Supplementary Material

Supplementary Materials includes seven tables as follows: The data of rarefaction curves (Tables S1). The data of weighted UniFrac distances (Tables S2). The data of principal coordinate analysis (Tables S3). The data of relative abundance of the top 10 bacterial phyla (Tables S4). The data of relative abundance of Proteobacteria subgroups (Tables S5). The data of relative abundance of the top 35 bacterial genera (Tables S6). The data of relative abundance of the top 10 bacerial genera (Tables S7).

Table S1

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Table S2

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Table S3

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Table S4

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Table S5

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Table S6

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Table S7

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Zheng, J., Li, J., Lan, Y. et al. Effects of Spartina alterniflora invasion on Kandelia candel rhizospheric bacterial community as determined by high-throughput sequencing analysis. J Soils Sediments 19, 332–344 (2019). https://doi.org/10.1007/s11368-018-2002-7

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Keywords

  • Community composition
  • High-throughput sequencing
  • Mangrove
  • Soil microbes
  • Spartina alterniflora