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Journal of Soils and Sediments

, Volume 18, Issue 5, pp 1853–1864 | Cite as

Soil pH rather than nutrients drive changes in microbial community following long-term fertilization in acidic Ultisols of southern China

  • Jia Liu
  • Ming Liu
  • Meng Wu
  • Chunyu Jiang
  • Xiaofen Chen
  • Zejiang Cai
  • Boren Wang
  • Jie Zhang
  • Taolin Zhang
  • Zhongpei Li
Soils, Sec 1 • Soil Organic Matter Dynamics and Nutrient Cycling • Research Article

Abstract

Purpose

Long-term intensive cultivation and heavy fertilization improve the nutritional conditions in acidic Ultisols, but also sharply accelerate soil acidification. However, the impact of such dramatic environmental changes on soil microorganisms is unclear. The aims of this work were to investigate the responses of microbial community composition and metabolic function to long-term fertilization, and to determine the key factors that primarily drive microbial changes in acidic Ultisols.

Materials and methods

A long-term fertilization experiment under a winter wheat–summer maize rotation was established in 1990 in acidic Ultisols of southern China. Soils were collected from four treatments in June 2014: (1) non-fertilization control (CK); (2) only N fertilization (N); (3) N, P, and K fertilization (NPK); and (4) NPK plus manure (NPKM; 70% of total N obtained from manure). The amount of N used in all N treatments was 300 kg N ha−1 year−1. The soil pH, cation exchange capacity (CEC), soil organic carbon (SOC), total nitrogen (TN), phosphorus (TP) and potassium (TK), available nitrogen (AN), phosphorus (AP), and potassium (AK) were measured. Soil microbial community composition and metabolic function were determined by phospholipid fatty acids analysis (PLFA) and community-level physiological profile (CLPP) method, respectively.

Results and discussion

Compared with CK, NPKM significantly increased total PLFA biomass and average well color development (AWCD); NPK increased total PLFA biomass by 2.2 times, but its AWCD was not significantly different from CK, indicating that microbial metabolic efficiency in NPK decreased. N decreased total PLFA biomass by 27.9%, while almost completely inhibiting metabolic activity. NPKM maintained microbial functional diversity indexes at similar levels as CK, while NPK and N significantly decreased microbial functional diversity indexes. Redundancy analysis (RDA) revealed that soil microbial community composition and metabolic pattern were more stably maintained by application of manure compared to chemical fertilizers. Soil pH showed the primary effect on microbial community composition, metabolic activity, and functional diversity indexes.

Conclusions

This research demonstrated that the negative effects of Ultisol acidification induced by long-term application of chemical N fertilizer on microorganisms overwhelmed the positive effects of soil nutrition improvement. The inhibiting effect of serious acidification on microbial metabolic function was stronger than that on community composition. Microorganisms live in a low active metabolic state to resist serious Ultisols acidification. Therefore, fertilization in acidic Ultisols should be based on the premise of preventing soil further acidification.

Keywords

Acidification CLPP Long-term fertilization PLFA Ultisols 

Notes

Acknowledgements

This study is supported by the National Basic Research Program (973 Program) of China (No. 2014CB441003) and the National Natural Science Foundation of China (Nos. 41661052 and 31660599). In addition, we thank the anonymous reviewers and editors for their helpful comments regarding the manuscript.

Supplementary material

11368_2018_1934_MOESM1_ESM.doc (62 kb)
ESM 1 (DOC 62 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jia Liu
    • 1
    • 2
    • 3
  • Ming Liu
    • 1
  • Meng Wu
    • 1
  • Chunyu Jiang
    • 1
  • Xiaofen Chen
    • 1
  • Zejiang Cai
    • 4
  • Boren Wang
    • 4
  • Jie Zhang
    • 5
  • Taolin Zhang
    • 1
    • 2
  • Zhongpei Li
    • 1
    • 2
  1. 1.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil ScienceChinese Academy of SciencesNanjingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Soil and Fertilizer & Resources and Environment InstituteJiangxi Academy of Agricultural SciencesNanchangChina
  4. 4.Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijingChina
  5. 5.Nanchang Institute of TechnologyNanchangChina

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