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Hazard assessment of polycyclic aromatic hydrocarbons in water and sediment in the vicinity of coalmines

Abstract

Purpose

Rivers feeding into the Loskop Dam, South Africa, pass through coal mining and heavily industrialised areas. Previous studies investigating mass mortalities of crocodile and fish in the river system, revealed the presence of organic compounds, including polycyclic aromatic hydrocarbons (PAHs), in their tissues.

Materials and methods

Samples were collected from nine sites within the dam in winter and summer. Liquid-liquid and microwave-assisted extraction was used for preconcentrating PAHs from water and sediment samples, respectively. Extracts were analysed by gas chromatography-mass spectrometry in the selected ion monitoring mode to determine the presence and levels of the 16 United States Environmental Protection Agency (US EPA)-priority PAHs.

Results and discussion

Significant levels of PAHs were found in both water and sediment samples. Concentrations were higher in sediments than in water, as well as in samples collected in winter. Levels of PAHs in sediments were generally higher than those reported by many researchers for other water bodies in industrialised areas. Zebrafish embryos were used to assess potential risks associated with the water and sediment, and to determine the effects of PAHs on aquatic life. Embryos, exposed to intact water and sediment samples, as well as to diluted sediment extracts, were monitored for 120 h post fertilisation. Sediment proved more toxic to zebrafish embryos than water, causing delayed embryo development and malformations.

Conclusions

These findings are alarming as they indicate that this water system is under stress. These findings can be typical of any water system situated in a coal mining and industrial region.

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References

  1. Brack W, Schirmer K, Erdinger L, Hollert H (2005) Effect-directed analysis of mutagens and ethoxyresorufin-o-deethylase inducers in aquatic sediments. Environ Toxicol Chem 24:2445–2458

    CAS  Article  Google Scholar 

  2. Bilek B (2009) Interest in carcinogenic PAHs increasing. Anal Consult 5:1–4

    Google Scholar 

  3. Braunbeck T, Bottcher M, Hollert H, Kosmehl T, Lammer E, Leist E, Rudolf M, Seitz N (2005) Towards an alternative for the acute fish LC50 test in chemical assessment: the fish embryo toxicity test goes multi-species-an update. Altex Altern Anim Ex 22:87–102

    Google Scholar 

  4. Braunbeck T, Lammer E (2006) Background document on fish embryo toxicity assays. Umwelt Bundes Amt UBA Contract No. 203 85 122: 1–40

  5. Brum DM, Cassella RJ, Netto ADP (2008) Multivariate optimization of a liquid–liquid extraction of the EPA-PAHs from natural contaminated waters prior to determination by liquid chromatography with fluorescence detection. Talanta 74:1392–1399

    CAS  Article  Google Scholar 

  6. De Villiers S, Mkwelo ST (2009) Has monitoring failed the Olifants River, Mpumalanga? Water SA 35:671–676

    Article  Google Scholar 

  7. Díaz-Moroles EN, Garza-Ulloa HJ, Castro-Ríos R, Ramírez-Villarreal EG, Barbarín-Castillo JM, De La Luz S-CM, Waksman-De Torres NA (2007) Comparison of the performance of two chromatographic and three extraction techniques for the analysis of PAHs in sources of drinking water. J Chromatogr Sci 45:57–62

    Article  Google Scholar 

  8. Embry MR, Belanger SE, Braunbeck TA, Galay-Burgos M, Halder M, Hinton DE, Léonard MA, Lillicrap A, Norberg-King T, Whale G (2010) The fish embryo toxicity test as an animal alternative method in hazard and risk assessment and scientific research. Aquat Toxicol 97:79–87

    CAS  Article  Google Scholar 

  9. French PW (1998) The impact of coal production on the sediment record of the Severn estuary. Environ Pollut 103:37–43

    CAS  Article  Google Scholar 

  10. Hahn ME (2002) Biomarkers and bioassays for detecting dioxin-like compounds in the marine environment. Sci Total Environ 289:46–69

    Article  Google Scholar 

  11. Hawkins AS, Billiard MS, Tabash PS, Brown SR, Hodson PV (2002) Altering cytochrome P4501A activity affects polycyclic aromatic hydrocarbon metabolism and toxicity in rainbow trout (Oncorhynchus mykiss). Environ Toxicol Chem 21:1845–1853

    CAS  Article  Google Scholar 

  12. Hollert H, Keiter S, Koning N, Rudolf M, Ulrich M, Braunbeck T (2003) A new sediment contact assay to assess particle-bound pollutants using zebrafish (Danio rerio) embryos. J Soils Sediments 3:197–207

    Article  Google Scholar 

  13. Huang L, Wang C, Zhang Y, LI J, Zhong Y, Zhou Y, Chen Y, Zuo Z (2012) Benzo(a)pyrene exposure influences the cardiac development and expression of cardiovascular relative genes in zebrafish (Danio rerio). Chemosphere 87:369–375

  14. Huchzermeyer KDA, Govender D, Pienaar D, Deacon AR (2011) Steatitis in wild sharptooth catfish, Clarius gariepinus (Burchell), in the Olifants and lower Letaba rivers in the Kruger National Park, South Africa. J Fish Dis 34:489–498

    CAS  Article  Google Scholar 

  15. Incardona JP, Collier KT, Scholz LN (2004) Defects in cardiac function precede morphological abnormalities in fish embryos exposed to polycyclic aromatic hydrocarbons. Toxicol Appl Pharmacol 196:191–205

    CAS  Article  Google Scholar 

  16. International Organization for Standardization ISO (1996) Water quality—determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)]. ISO 7346–3 Flow-through method http://www.iso.org.

  17. Itoh N, Numata M, Aoyagi Y, Yarita T (2008) Comparison of low-level polycyclic aromatic hydrocarbons in sediment revealed by Soxhlet extraction, microwave-assisted extraction, and pressurized liquid extraction. Anal Chim Acta 612:44–52

    CAS  Article  Google Scholar 

  18. Jones KC, De Voogt P (1999) Persistent organic pollutants (POPs): state of the science. Environ Pollut 100:209–221

    CAS  Article  Google Scholar 

  19. Kampa M, Castanas E (2008) Human health effects of air pollution. Environ Pollut 151:362–367

    CAS  Article  Google Scholar 

  20. Lammer E, Carr GJ, Wendler K, Rawlings JM, Belanger SE, Braunbeck T (2009a) Is the fish embryo toxicity test (FET) with the zebrafish (Danio rerio) a potential alternative for the fish acute toxicity test? Comp Biochem Physiol, Part C 149:196–209

    CAS  Google Scholar 

  21. Lammer E, Kamp HG, Hisgen V, Koch M, Reinhard D, Salinas ER, Wendler K, Zok S, Braunbeck T (2009b) Development of flow-through system for the fish embryo toxicity test (FET) with the zebrafish (Danio rerio). Toxicol in Vitro 23:1436–1442

    CAS  Article  Google Scholar 

  22. Liu Y, Chen L, Huang Q, Li W, Tang Y, Zhao J (2009) Source apportionment of polycyclic aromatic hydrocarbons (PAHs) in surface sediments of the Huangpu River, Shanghai, China. Sci Total Environ 407:2931–2938

    CAS  Article  Google Scholar 

  23. Lopez-Avila V, Young R, Beckert FW (1994) Microwave-assisted extraction of organic compounds from standard reference soils and sediments. Anal Chem 66:1097–1106

    CAS  Article  Google Scholar 

  24. MacDonald DD, Ingersoll CG, Smorong DE, Lindskoog RA, Sloane G, Biernacki T (2003) Development and evaluation of numerical sediment quality guidelines for Florida inland waters. Department of Environmental Protection, Florida

    Google Scholar 

  25. Marinov D, Dueri S, Puillat I, Carafa R, Jurado E, Berrojalbiz N, Dachs J, Zaldivar J (2009) Integrated modelling of polycyclic aromatic hydrocarbons in the marine environment: coupling of hydrodynamic, fate and transport, bioaccumulation and planktonic food-web models. Mar Pollut Bull 58:1554–1561

    CAS  Article  Google Scholar 

  26. McCarthy TS, Pretorius K (2009) Coal mining on the Highveld and its implications for future water quality in the Vaal River system. The International Mine Water Conference, Pretoria, South Africa

  27. Meyer W, Seiler TB, Reininghaus M, Schwarzbauer J, Püttmann W, Hollert H, Achten C (2013) Limited waterborne acute toxicity of native polycyclic aromatic compounds from coals of different types compared to their total hazard potential. Environ Sci Technol 47:11766–11775

    CAS  Article  Google Scholar 

  28. Meyer W, Seiler TB, Schwarzbauer J, Püttmann W, Hollert H, Achten C (2014) Polar polycyclic aromatic compounds from different coal types show varying mutagenic potential, EROD induction and bioavailability depending on coal rank. Sci Total Environ 494-495:320–328

    CAS  Article  Google Scholar 

  29. Mhadhbi L, Boumaiza M, Beiras R (2010) A standard ecotoxicological bioassay using early life stages of the marine fish Psetta maxima. Aquat Living Resour 23:209–216

    CAS  Article  Google Scholar 

  30. Milan DJ, Peterson TA, Ruskin JN, Randall TP, Macrae CA (2003) Drugs that induce repolarization abnormalities cause bradycardia in zebrafish. Circulation; http://circ.ahajournals.org/content/107/10/1355.short [Accessed: 22/05/2012]

  31. Miller J, Miller JC (2005) Statistics and chemometrics for analytical chemistry, 5th edn. Ashford Colour Press, Gosport

    Google Scholar 

  32. Moyo S, Mujuru M, McCrindle RI, Mokgalaka NS (2011) Environmental implications of material leached from coal. J Environ Monit 13:1485–1494

    Article  Google Scholar 

  33. Moyo S, McCrindle RI, Mokgalaka NS, Myburgh JG, Mujuru M (2013) Source apportionment of polycyclic aromatic hydrocarbons in sediments from polluted rivers. Pure Appl Chem 85:2175–2196

    CAS  Article  Google Scholar 

  34. Mumtaz M, George J (1995) Toxicological profile for polycyclic aromatic hydrocarbons. http://www.atsdr.cdc.gove/toxfaq.html [Accessed: 01/03/2012]

  35. Mwape P, Roberts MJ, Mokwena E, Tjatjie T, Phale M (2005) Part one: South Africa’s mineral industry general review: South Africa’s mineral industry (2004/2005). Department of Minerals and Energy, Pretoria

    Google Scholar 

  36. OECD guideline for the testing of chemicals (2012) http://www.oecd.org/env/ehs/testing/2012-07-09_Draft_FET_TG_v8_FINAL.pdf [Accessed: 01/08/2012]

  37. Persoone G, Marsalek B, Blinova I, Torokne A, Zarina D, Manusadzianas L, Nalecz-Jawecki G, Tofan L, Stepanova N, Tothova L, Kolar B (2003) A practical and user friendly toxicity classification system with microbiotests for natural waters and wastewaters. Environ Toxicol 18:395–402

    CAS  Article  Google Scholar 

  38. Ramalhosa MJ, Paiga P, Delerue-Matos C, Oliveira MBPP (2009) Analysis of polycyclic aromatic hydrocarbons in fish: evaluation of a quick, easy, cheap, effective, rugged, and safe extraction method. J Sep Sci 32:3529–3538

    CAS  Article  Google Scholar 

  39. Ramalhosa MJ, Paiga P, Morais S, Sousa AMM, Gonçalves MP, Delerue-Matos C, Oliveira MBPP (2012) Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction. Food Chem 135:234–242

    CAS  Article  Google Scholar 

  40. Strähle U, Scholz S, Geisler R, Greiner P, Hollert H, Rastegar S, Schumacher A, Selderslaghs I, Weiss C, Witters H, Braunbeck T (2012) Zebrafish embryos as an alternative to animal experiments—a commentary on the definition of the onset of protected life stages in animal welfare regulations. Reprod Toxicol 33:128–132

    Article  Google Scholar 

  41. Selderslaghs IWT, Blust R, Witters HE (2011) Feasibility study of the zebrafish assay as an alternative method to screen for developmental toxicity and embryotoxicity using a training set of 27 compounds. Reprod Toxicol 33:142–154

    Article  Google Scholar 

  42. Strmac M, Oberemm A, Braunbeck T (2002) Effects of sediment eluates and extracts from differently polluted small rivers on zebrafish embryos and larvae. J Fish Biol 61:24–38

    Article  Google Scholar 

  43. UNESCO/WHO/UNEP (1996) Water quality assessments-a guide to use of biota, sediments and water in environmental monitoring. WHO, Cambridge

    Google Scholar 

  44. Van Vuuren L (2009) Experts unite to save abused river from extinction. The Water Wheel Jan/Feb:14–17

  45. US EPA (2001) Methods for collection, storage and manipulation of sediments for chemical and toxicological analyses: technical manual. US EPA: Washington DC, USA, Vol. EPA 823-B-01-002

  46. US EPA (2007) Method 610-polynuclear aromatic hydrocarbons. http://water.epa.gov/scitech/methods/cwa/organics [Accessed: 03/02/2015]

  47. US EPA (1996a) Separatory funnel liquid-liquid extraction. http://www.epa.gov/osw/hazard/testmethods [Accessed 03/02/2015]

  48. US EPA (1996b) Silica gel cleanup. http://www.epa.gov/solidwaste/hazard/testmethods [Accessed: 03/02/2015]

  49. West DM, Holler JF, Crouch SR, Skoog DA (2014) Fundamentals of analytical chemistry. Texas

  50. Yang K, Zhu L, Xing B (2006) Adsorption of polycyclic aromatic hydrocarbons by carbon nanomaterials. Environ Sci Technol 40:1855–1861

    CAS  Article  Google Scholar 

  51. Yende SS (2012) Flagrant coal mining threatens food security: Highveld region could become a total wasteland once coal reserves have been fully exploited and mining ceases, say experts. City Press 27 May

  52. Zielke H, Seiler T, Niebergall S, Leist E, Brinkmann M, Spira D, Streck G, Brack W, Feiler U, Braunbeck T, Hollert H (2011) The impact of extraction methodologies on the toxicity of sediments in the zebrafish (Danio rerio) embryo test. J Soils Sediments 11:352–363

    CAS  Article  Google Scholar 

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Acknowledgments

The authors acknowledge funding from the National Research Foundation (of South Africa) and the staff of Mpumalanga tourism and parks agency situated at Loskop Dam Nature Reserve. We thank the Aquatic Ecology and Toxicology Section, University of Heidelberg, Germany for the assistance with the establishment of the zebrafish embryo toxicity model in our laboratory

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Correspondence to Robert I. McCrindle.

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Responsible editor: Arnold V Hallare

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Seopela, M.P., McCrindle, R.I., Combrinck, S. et al. Hazard assessment of polycyclic aromatic hydrocarbons in water and sediment in the vicinity of coalmines. J Soils Sediments 16, 2740–2752 (2016). https://doi.org/10.1007/s11368-016-1499-x

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Keywords

  • Polycyclic aromatic hydrocarbons
  • Hazard assessment
  • Sediment
  • Water
  • Zebrafish embryo assay