Journal of Soils and Sediments

, Volume 17, Issue 1, pp 23–34 | Cite as

Nitrogen addition impacts on the emissions of greenhouse gases depending on the forest type: a case study in Changbai Mountain, Northeast China

Soils, Sec 1 • Soil Organic Matter Dynamics and Nutrient Cycling • Research Article



Anthropogenic-induced greenhouse gas (GHG) emission rates derived from the soil are influenced by long-term nitrogen (N) deposition and N fertilization. However, our understanding of the interplay between increased N load and GHG emissions among soil aggregates is incomplete.

Materials and methods

Here, we conducted an incubation experiment to explore the effects of soil aggregate size and N addition on GHG emissions. The soil aggregate samples (0–10 cm) were collected from two 6-year N addition experiment sites with different vegetation types (mixed Korean pine forest vs. broad-leaved forest) in Northeast China. Carbon dioxide (CO2), nitrous oxide (N2O), and methane (CH4) production were quantified from the soil samples in the laboratory using gas chromatography with 24-h intervals during the incubation (at 20 °C for 168 h with 80 % field water capacity).

Results and discussion

The results showed that the GHG emission/uptake rates were significantly higher in the micro-aggregates than in the macro-aggregates due to the higher concentration of soil bio-chemical properties (DOC, MBC, NO3 , NH4 +, SOC and TN) in smaller aggregates. For the N addition treatments, the emission/uptake rates of GHG decreased after N addition across aggregate sizes especially in mixed Korean pine forest where CO2 emission was decreased about 30 %. Similar patterns in GHG emission/uptake rates expressed by per soil organic matter basis were observed in response to N addition treatments, indicating that N addition might decrease the decomposability of SOM in mixed Korean pine forest. The global warming potential (GWP) which was mainly contributed by CO2 emission (>98 %) decreased in mixed Korean pine forest after N addition but no changes in broad-leaved forest.


These findings suggest that soil aggregate size is an important factor controlling GHG emissions through mediating the content of substrate resources in temperate forest ecosystems. The inhibitory effect of N addition on the GHG emission/uptake rates depends on the forest type.


Greenhouse gas N deposition Soil aggregate Soil organic carbon 



This work was funded by grants from the strategic priority research program of the Chinese Academy of Sciences (XDA05020300), the National Natural Science Foundation of China (41330530, 41430639, 31470522).


  1. Allison SD (2006) Soil minerals and humic acids alter enzyme stability: implications for ecosystem processes. Biogeochemistry 81:361–373CrossRefGoogle Scholar
  2. Aoyama M, Angers DA, N’Dayegamiye A, Bissonnette N (1999) Protected organic matter in water-stable aggregates as affected by mineral fertilizer end manure applications. Can J Soil Sci 79:419–425CrossRefGoogle Scholar
  3. Bai E, Li W, Li SL, Sun JF, Peng B, Dai WW, Jiang P, Han SJ (2014) Pulse increase of soil N2O emission in response to N addition in a temperate forest on Mt Changbai, northeast China. Plos One 9 doi:10.1371/journal.pone.0102765Google Scholar
  4. Bandyopadhyay KK, Lal R (2014) Effect of land use management on greenhouse gas emissions from water stable aggregates. Geoderma 232:363–372CrossRefGoogle Scholar
  5. Bodelier PLE, Laanbroek HJ (2004) Nitrogen as a regulatory factor of methane oxidation in soils and sediments. FEMS Microbiol Ecol 47:265–277CrossRefGoogle Scholar
  6. Bonan GB (2008) Forests and climate change: forcings, feedbacks, and the climate benefits of forests. Science 320:1444–1449CrossRefGoogle Scholar
  7. de Vries W, Du EZ, Butterbach-Bahl K (2014) Short and long-term impacts of nitrogen deposition on carbon sequestration by forest ecosystems. Curr Opin Environ Sustain 9–10:90–104CrossRefGoogle Scholar
  8. Diba F, Shimizu M, Hatano R (2011) Effects of soil aggregate size, moisture content and fertilizer management on nitrous oxide production in a volcanic ash soil. Soil Sci Plant Nutr 57:733–747CrossRefGoogle Scholar
  9. Dixon RK, Brown S, Houghton RA, Solomon AM, Trexler MC, Wisniewski J (1994) Carbon pools and flux of global forest ecosystems. Science 263:185–190CrossRefGoogle Scholar
  10. Elliott ET, Paustian K, Frey SD (1996) Modeling the measurable or measuring the modelable: A hierarchical approach to isolating meaningful soil organic matter fractionations. In: David S. Powlson PS, Jo U. Smith (ed) Evaluation of Soil Organic Matter Models. NATO ASI Series, vol 38. Springer Berlin Heidelberg, pp 161–179.Google Scholar
  11. Flessa H, Wild U, Klemisch M, Pfadenhauer J (1998) Nitrous oxide and methane fluxes from organic soils under agriculture. Eur J Soil Sci 49:327–335CrossRefGoogle Scholar
  12. Frey SD, Ollinger S, Nadelhoffer K, Bowden R, Brzostek E, Burton A, Caldwell BA, Crow S, Goodale CL, Grandy AS, Finzi AC, Kramer MG, Lajtha K, LeMoine J, Martin M, McDowell WH, Minocha R, Sadowsky JJ, Templer PH, Wickings K (2014) Chronic nitrogen additions suppress decomposition and sequester soil carbon in temperate forests. Biogeochemistry 121:305–316CrossRefGoogle Scholar
  13. Galloway JN, Dentener FJ, Capone DG, Boyer EW, Howarth RW, Seitzinger SP, Asner GP, Cleveland CC, Green PA, Holland EA, Karl DM, Michaels AF, Porter JH, Townsend AR, Vorosmarty CJ (2004) Nitrogen cycles: past, present, and future. Biogeochem 70:153–226CrossRefGoogle Scholar
  14. Gough CM, Seiler JR (2004) Belowground carbon dynamics in loblolly pine (Pinus taeda) immediately following diammonium phosphate fertilization. Tree Physiol 24:845–851CrossRefGoogle Scholar
  15. Gulledge J, Hrywna Y, Cavanaugh C, Steudler PA (2004) Effects of long-term nitrogen fertilization on the uptake kinetics of atmospheric methane in temperate forest soils. FEMS Microbiol Ecol 49:389–400CrossRefGoogle Scholar
  16. IPCC (2007) Climate change 2007: the physical science basis, Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USAGoogle Scholar
  17. Jastrow JD, Amonette JE, Bailey VL (2007) Mechanisms controlling soil carbon turnover and their potential application for enhancing carbon sequestration. Climatic Change 80:5–23CrossRefGoogle Scholar
  18. Kimura SD, Melling L, Goh KJ (2012) Influence of soil aggregate size on greenhouse gas emission and uptake rate from tropical peat soil in forest and different oil palm development years. Geoderma 185:1–5CrossRefGoogle Scholar
  19. Lal R (2008) Carbon sequestration. Philosophical transactions of the Royal Society B-Biological Sciences 363:815–830CrossRefGoogle Scholar
  20. Lee KH, Jose S (2003) Soil respiration, fine root production, and microbial biomass in cottonwood and loblolly pine plantations along a nitrogen fertilization gradient. Forest Ecol Manage 185:263–273CrossRefGoogle Scholar
  21. Li W, Bai E, Li SL, Sun JF, Peng B, Jiang P (2013) Effects of nitrogen addition and precipitation change on soil methane and carbon dioxide fluxes. Chinese Journal of Ecology 32:1947–1958Google Scholar
  22. Liu LL, Greaver TL (2010) A global perspective on belowground carbon dynamics under nitrogen enrichment. Ecol Lett 13:819–828CrossRefGoogle Scholar
  23. Liu XJ et al (2013) Enhanced nitrogen deposition over China. Nature 494:459–462CrossRefGoogle Scholar
  24. Lu M, Zhou XH, Luo YQ, Yang YH, Fang CM, Chen JK, Li B (2011) Minor stimulation of soil carbon storage by nitrogen addition: a meta-analysis. Agr Ecosyst Environ 140:234–244CrossRefGoogle Scholar
  25. Maljanen M, Jokinen H, Saari A, Strommer R, Martikainen PJ (2006) Methane and nitrous oxide fluxes, and carbon dioxide production in boreal forest soil fertilized with wood ash and nitrogen. Soil Use Manage 22:151–157CrossRefGoogle Scholar
  26. Mangalassery S, Sjogersten S, Sparkes DL, Sturrock CJ, Mooney SJ (2013) The effect of soil aggregate size on pore structure and its consequence on emission of greenhouse gases. Soil Till Res 132:39–46CrossRefGoogle Scholar
  27. Moore TR, Dalva M (1997) Methane and carbon dioxide exchange potentials of peat soils in aerobic and anaerobic laboratory incubations. Soil Biol Biochem 29:1157–1164CrossRefGoogle Scholar
  28. Mueller CW, Schlund S, Prietzel J, Kogel-Knabner I, Gutsch M (2012) Soil aggregate destruction by ultrasonication increases soil organic matter mineralization and mobility. Soil Sci Soc Am J 76:1634–1643CrossRefGoogle Scholar
  29. Oades JM, Waters AG (1991) Aggregate hierarchy in soils. Aust J Soil Res 29:815–828CrossRefGoogle Scholar
  30. Paustian K, Six J, Elliott ET, Hunt HW (2000) Management options for reducing CO2 emissions from agricultural soils. Biogeochemistry 48:147–163CrossRefGoogle Scholar
  31. Plaza-Bonilla D, Cantero-Martinez C, Alvaro-Fuentes J (2014) Soil management effects on greenhouse gases production at the macroaggregate scale. Soil Biol Biochem 68:471–481CrossRefGoogle Scholar
  32. Ranjard L, Richaume AS (2001) Quantitative and qualitative microscale distribution of bacteria in soil. Res Microbiol 152:707–716CrossRefGoogle Scholar
  33. Sey BK, Manceur AM, Whalen JK, Gregorich EG, Rochette P (2008) Small-scale heterogeneity in carbon dioxide, nitrous oxide and methane production from aggregates of a cultivated sandy-loam soil. Soil Biol Biochem 40:2468–2473CrossRefGoogle Scholar
  34. Shrestha RK, Strahm BD, Sucre EB, Holub SM, Meehan N (2014) Fertilizer management, parent material, and stand age influence forest soil greenhouse gas fluxes. Soil Sci Soc Am J 78:2041–2053CrossRefGoogle Scholar
  35. Shrestha RK, Strahm BD, Sucre EB (2015) Greenhouse gas emissions in response to nitrogen fertilization in managed forest ecosystems. New Forests 46:167–193CrossRefGoogle Scholar
  36. Six J, Paustian K (2014) Aggregate-associated soil organic matter as an ecosystem property and a measurement tool. Soil Biol Biochem 68:A4–A9CrossRefGoogle Scholar
  37. Six J, Elliott ET, Paustian K, Doran JW (1998) Aggregation and soil organic matter accumulation in cultivated and native grassland soils. Soil Sci Soc Am J 62:1367–1377CrossRefGoogle Scholar
  38. Sollins P, Homann P, Caldwell BA (1996) Stabilization and destabilization of soil organic matter: mechanisms and controls. Geoderma 74:65–105CrossRefGoogle Scholar
  39. Stiehl-Braun PA, Hartmann AA, Kandeler E, Buchmann N, Niklaus PA (2011) Interactive effects of drought and N fertilization on the spatial distribution of methane assimilation in grassland soils. Glob Change Biol 17:2629–2639CrossRefGoogle Scholar
  40. Tans PP, Fung IY, Takahashi T (1990) Observational constraints on the global atmospheric CO2 budget. Science 247:1431–1438CrossRefGoogle Scholar
  41. Tao BX, Song CC (2013) Temperature sensitivity of carbon dioxide production in aggregates and their responses to nitrogen addition in a freshwater marsh, Sanjiang Plain. Soil Science and Plant Nutrition 59:953–960CrossRefGoogle Scholar
  42. Uchida Y, Clough TJ, Kelliher FM, Sherlock RR (2008) Effects of aggregate size, soil compaction, and bovine urine on N2O emissions from a pasture soil. Soil Biol Biochem 40:924–931CrossRefGoogle Scholar
  43. von Lutzow M, Kogel-Knabner I, Ekschmittb K, Flessa H, Guggenberger G, Matzner E, Marschner B (2007) SOM fractionation methods: relevance to functional pools and to stabilization mechanisms. Soil Biol Biochem 39:2183–2207CrossRefGoogle Scholar
  44. Wang B, Jiang Y, Wei XH, Zhao GD, Guo H, Bai XL (2011) Effects of forest type, stand age, and altitude on soil respiration in subtropical forests of China. Scan J Forest Res 26:40–47CrossRefGoogle Scholar
  45. Wang CG, Han SJ, Zhou YM, Yan CF, Cheng XB, Zheng XB, Li MH (2012) Responses of fine roots and soil N availability to short-term nitrogen fertilization in a broad-leaved Korean pine mixed forest in northeastern China. Plos One 7:e31042. doi: 10.1371/journal.pone.0031042 CrossRefGoogle Scholar
  46. Whittenbury R, Phillips KC, Jf W (1970) Enrichment, isolation and some properties of methane-utilizing bacteria. J Gen Microbiol 61:205–218CrossRefGoogle Scholar
  47. Wu HH, Xu XK, Duan CT, Li TS, Cheng WG (2015) Effect of vegetation type, wetting intensity, and nitrogen supply on external carbon stimulated heterotrophic respiration and microbial biomass carbon in forest soils. Science China-Earth Sciences 58:1446–1456CrossRefGoogle Scholar
  48. Young IM, Crawford JW, Nunan N, Otten W, Spiers A (2008) Microbial distribution in soils: physics and scaling. In: Sparks DL (ed) Advances in agronomy, vol 100. Advances in agronomy., pp 81–121. doi: 10.1016/s0065-2113(08)00604-4 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Zhijie Chen
    • 1
    • 2
  • Heikki Setälä
    • 3
  • Shicong Geng
    • 1
    • 2
  • Shijie Han
    • 4
  • Shuqi Wang
    • 1
  • Guanhua Dai
    • 5
  • Junhui Zhang
    • 1
  1. 1.Key Laboratory of Forest Ecology and Management, Institute of Applied EcologyChinese Academy of SciencesShenyangChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Department of Environmental SciencesUniversity of HelsinkiLahtiFinland
  4. 4.Institute of Applied Ecology, Chinese Academy of SciencesShenyangChina
  5. 5.Changbai Mountains Forest Ecosystem Research StationAntuChina

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