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The foliar spray of Rhodopseudomonas palustris grown under Stevia residue extract promotes plant growth via changing soil microbial community

  • Soils, Sec 3 • Remediation and Management of Contaminated or Degraded Lands • Research Article
  • Published:
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Abstract

Purpose

The extract of Stevia residue is an ideal substitute for cultivation of the purple nonsulfur bacterium, like Rhodopseudomonas palustris (R. palustris). But the influence of R. palustris grown under residue extract on its downstream application is still not well-characterized. The objective of this study was to assess the effect of foliar spray of R. palustris grown under Stevia residue extract on the plant growth and soil microbial properties.

Materials and methods

A pot experiment was carried out under the greenhouse condition, consisting of four treatments varying in the sprayed substances: sterilized water (control), R. palustris grown under the chemical medium supplemented with L-tryptophan (SyT), R. palustris grown under Stevia residue extract supplemented with L-tryptophan (ExT), and R. palustris grown under Stevia residue extract supplemented with NH4Cl (ExT). The net photosynthesis rate of the uppermost leaves was measured with a portable photosynthesis system. Soil microbial activity was analyzed by microcalorimetry. Soil bacterial community components were determined by real-time quantitative PCR (qPCR) and high-throughput sequencing techniques.

Results and discussion

Compared with SyT, the R. palustris grown under Stevia residue extract not only improved the plant biomass and the net photosynthetic rate to a large extent, but also increased soil microbial metabolic activity and altered community compositions as well. The treatments receiving R. palustris, especially ExT and ExN, increased the relative abundances of some functional guilds involved in C turnover and nutrient cycling in soil, including Acidobacteria, Actinobacteria, Proteobacteria, Gemmatimonadaetes, Nitrospirae, and Planctomycetes.

Conclusions

R. palustris grown under the Stevia residue extract showed advantages over that under the chemical medium on both plant growth and soil microbial properties. One of the possible reasons could result from the increases in microbial activity and several bacterial keystone guilds involved into C and nutrient cycling, both of which potentially contribute to the improved plant growth. The results would be conducive to the downstream application of R. palustris in an economical way.

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Acknowledgments

This work was supported jointly by the National Natural Science Foundation of China (41501264 and 41571286), the Natural Science Foundation of Jiangsu Province (BK20140991), the Research Fund of State Key Laboratory of Soil and Sustainable Agriculture, Nanjing Institute of Soil Science, Chinese Academy of Science (Y412201441), and the Startup Foundation for Introducing Talent of Nanjing University of Information Science and Technology(NUIST).

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Authors and Affiliations

  1. International Center for Ecology, Meteorology and Environment (IceMe), School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing, 210044, China

    Jiangbing Xu, Yanling Wang, Xiaosan Luo & Jianwu Tang

  2. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Jiangbing Xu, Youzhi Feng & Xiangui Lin

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  1. Jiangbing Xu
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  2. Youzhi Feng
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  3. Yanling Wang
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  4. Xiaosan Luo
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  5. Jianwu Tang
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  6. Xiangui Lin
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Correspondence to Xiangui Lin.

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Responsible editor: Chengrong Chen

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Xu, J., Feng, Y., Wang, Y. et al. The foliar spray of Rhodopseudomonas palustris grown under Stevia residue extract promotes plant growth via changing soil microbial community. J Soils Sediments 16, 916–923 (2016). https://doi.org/10.1007/s11368-015-1269-1

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  • DOI: https://doi.org/10.1007/s11368-015-1269-1

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