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Changes of labile and recalcitrant carbon pools under nitrogen addition in a city lawn soil

  • SOILS, SEC 2 • GLOBAL CHANGE, ENVIRON RISK ASSESS, SUSTAINABLE LAND USE • RESEARCH ARTICLE
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Abstract

Purpose

Carbon (C) flux is largely controlled by the highly bio-reactive labile C (LC) pool, while long-term C storage is determined by the recalcitrant C (RC) pool. Soil nitrogen (N) availability may considerably affect changes of these pools. The aim of this study was to investigate the effects of N treatments on soil LC and RC pools.

Materials and methods

A field experiment was conducted in a city lawn soil for 600 days with three N treatments, i.e., the control (0 kg N ha−1 year−1), low-N (100 kg N ha−1 year−1), and high-N (200 kg N ha−1 year−1) treatments. As the N source, NH4NO3 solution was added to soil surface monthly. Measurements of LC, RC, and other soil biochemical properties, including pH, soil respiration rates, microbial biomass, and enzymes activities, were taken during the experiment period.

Results and discussion

The low-N and high-N treatments increased 6.3 and 13% of the LC pool, respectively, which was caused by decreased microbial biomass and soil respiration rates under the N treatments. By contrary, the low-N and high-N treatments decreased 5.9 and 12% of the RC pool, respectively. The N addition treatments enhanced phenol oxidase activities. The enhanced oxidase activities decreased new RC input and the increased dissolved organic C stimulated RC pool decomposition. The LC and RC pools were highly influenced by the N treatments, whereas effect of the N treatments on soil organic C was not significant. The N addition treatments also caused soil acidification and reduced bacterial biomass proportion in the soil microbial composition.

Conclusions

The N addition increased the LC pool but decreased the RC pool in the soil. These changes should greatly impact soil long-term C storage.

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References

  • Allison SD, Vitousek PM (2004) Extracellular enzyme activities and carbon chemistry as drivers of tropical plant litter decomposition. Biotropica 36:285–296

    Google Scholar 

  • Allison SD, Czimczik CI, Treseder KK (2008) Microbial activity and soil respiration under nitrogen additions in Alaskan boreal forest. Global Change Biol 14:1156–1168

    Article  Google Scholar 

  • Alvarez CR, Alvarez R, Grigera S, Lavado RS (1998) Associations between organic matter fractions and the active soil microbial biomass. Soil Biol Biochem 30(6):767–773

    Article  CAS  Google Scholar 

  • Bardgett RD, McAlister E (1999) The measurement of soil fungal: bacterial biomass ratios as an indicator of ecosystem self-regulation in temperate meadow grasslands. Biol Fert Soils 29:282–290

    Article  Google Scholar 

  • Bardgett RD, Lovell RD, Hobbs PJ, Jarvis SC (1999) Seasonal changes in soil microbial communities along a fertility gradient of temperate grasslands. Soil Biol Biochem 31:1021–1030

    Article  CAS  Google Scholar 

  • Bi J, Zhang NL, Liang Y, Yang HJ, Ma KP (2012) Interactive effects of water and nitrogen addition on soil microbial communities in a semiarid steppe. J Plant Ecol 5:320–329

    Article  Google Scholar 

  • Bossuyt H, Denef K, Six J, Frey SD, Merckx R, Paustian K (2001) Influence of microbial populations and residue quality on aggregate stability. Appl Soil Ecol 16:195–208

    Article  Google Scholar 

  • Bottomley PJ (1994) Light microscopic methods for studying soil microorganisms. In: Weaver RW (ed) Methods of soil analysis. Part 2. Microbiological and biochemical properties. Soil Science Society of America, Madison, pp 81–105

  • Brookes PC, Landman A, Pruden G, Jenkinson DS (1985) Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biol Biochem 17:837–842

    Article  CAS  Google Scholar 

  • Burton AJ, Pregitzer KS, Crawford JN, Zogg GP, Zak DR (2004) Simulated chronic NO3 − deposition reduces soil respiration in northern hardwood forests. Global Change Biol 10(7):1080–1091

    Article  Google Scholar 

  • Busse MD, Sanchez FG, Ratcliff AW, Butnor JR, Carter EA, Powers RE (2009) Soil carbon sequestration and changes in fungal and bacterial biomass following incorporation of forest residues. Soil Biol Biochem 41:220–227

    Article  CAS  Google Scholar 

  • Carrillo Y, Pendall E, Dijkstra FA, Morgan JA, Newcomb JM (2011) Response of soil organic matter pools to elevated CO2 and warming in a semi-arid grassland. Plant Soil 347:339–350

    Article  CAS  Google Scholar 

  • Cheng L, Leavitt SW, Kimball BA, Pinter JPJ, Ottman MJ, Matthias A, Wall GW, Brooks T, Williams DG, Thompson TL (2007) Dynamics of labile and recalcitrant soil carbon pools in a sorghum free-air CO2 enrichment (FACE) agro ecosystem. Soil Biol Biochem 39:2250–2263

    Article  CAS  Google Scholar 

  • Collins HP, Elliott ET, Paustian K, Bundy LG, Dick WA, Huggins DR, Smucker AJM, Paul EA (2000) Soil carbon pools and fluxes in long-term corn belt agroecosystems. Soil Biol Biochem 32:157–168

    Article  CAS  Google Scholar 

  • Currey PM, Johnson D, Sheppard LJ, Leith ID, Toberman H, Wal RV, Dawson LA, Artz RRE (2010) Turnover of labile and recalcitrant soil carbon differ in response to nitrate and ammonium deposition in an ombrotrophic peat land. Global Change Biol 16:2307–2321

    Article  Google Scholar 

  • DeForest JL, Zak DR, Pregitzer KS, Burton AJ (2004) Atmospheric nitrate deposition, microbial community composition, and enzyme activity in northern hardwood forests. Soil Sci Soc Am J 68:132–138

    Article  CAS  Google Scholar 

  • Emmett BA (1999) The impact of nitrogen on forest soils and feedbacks on tree growth. Water Air Soil Poll 116(1–2):65–74

    Article  CAS  Google Scholar 

  • Fog K (1988) The effect of added nitrogen on the rate of decomposition of organic matter. Biol Rev 63:433–462

    Article  Google Scholar 

  • Fontaine S, Barot S, Barre P, Bdioui N, Mary B, Rumpel C (2007) Stability of organic carbon in deep soil layers controlled by fresh carbon supply. Nature 450:277–280

    Article  CAS  Google Scholar 

  • Galantini J, Rosell R (2006) Long-term fertilization effects on soil organic matter quality and dynamics under different production systems in semi-arid Pampean soils. Soil Till Res 87:72–79

    Article  Google Scholar 

  • George E, Kircher S, Schwarz P, Tesar A, Seith B (1999) Effect of varied soil nitrogen supply on growth and nutrient uptake of young Norway spruce plants grown in a shaded environment. J Plant Nutr Soil Sci 162(3):301–307

    Article  CAS  Google Scholar 

  • Giesler R, Hogberg MN, Strobel BW, Richter A, Nordgren A, Hogberg P (2007) Production of dissolved organic carbon and low-molecular weight organic acids in soil solution driven by recent tree photosynthate. Biogeochemistry 84(1):1–12

    CAS  Google Scholar 

  • Gong W, Yan X, Wang J (2012) The effect of chemical fertilizer on soil organic carbon renewal and CO2 emission—a pot experiment with maize. Plant Soil 353:85–94

    Article  CAS  Google Scholar 

  • Groffman PM, Williams CO, Pouyat RV, Band LE, Yesilonis ID (2009) Nitrate leaching and nitrous oxide flux in urban forests and grasslands. J Environ Qual 38:1848–1860

    Article  CAS  Google Scholar 

  • Helmisaari HS, Hallvacken L (1999) Fine-root biomass and necromass in limed and fertilized Norway spruce (Picea abies (L.) Karst.) stands. For Ecol Manag 119:99–110

    Article  Google Scholar 

  • Helmisaari HS, Saarsalmi A, Kukkola M (2009) Effects of wood ash and nitrogen fertilization on fine root biomass and soil and foliage nutrients in a Norway spruce stand in Finland. Plant Soil 314(1–2):121–132

    Article  CAS  Google Scholar 

  • Hobbie SE, Nadelhoffer KJ, Högberg P (2002) A synthesis: the role of nutrients as constraints on carbon balances in boreal and arctic regions. Plant Soil 242:163–170

    Article  CAS  Google Scholar 

  • Högberg MN, Högberg P (2002) Extramatrical ectomycorrhizal mycelium contributes one-third of microbial biomass and produces, together with associated roots, half the dissolved organic carbon in a forest soil. New Phytol 154(3):791–795

    Article  Google Scholar 

  • Hu S, Coleman DC, Carroll CR, Hendrix PF, Beare MH (1997) Labile soil carbon pools in subtropical forest and agricultural ecosystems as influenced by management practices and vegetation types. Agr Ecosyst Environ 65:69–78

    Article  CAS  Google Scholar 

  • Huang Z, Clinton PW, Baisden WT, Davis MR (2011) Long-term nitrogen additions increased surface soil carbon concentration in a forest plantation despite elevated decomposition. Soil Biol Biochem 43:302–307

    Article  CAS  Google Scholar 

  • Ingham ER, Klein DA (1984) Soil fungi: relationships between hyphal activity and staining with fluorescein diacetate. Soil Biol Biochem 16:273–278

    Article  CAS  Google Scholar 

  • Iyyemperumal K, Shi W (2008) Soil enzyme activities in two forage systems following application of different rates of swine lagoon effluent or ammonium nitrate. Appl Soil Ecol 38:128–136

    Article  Google Scholar 

  • Jim CY, Chen WY (2006) Perception and attitude of residents toward urban green spaces in Guangzhou (China). Environ Manage 38:338–349

    Article  CAS  Google Scholar 

  • Johnsona D, Leakea JR, Leea JA, Campbell CD (1998) Changes in soil microbial biomass and microbial activities in response to 7 years simulated pollutant nitrogen deposition on a heathland and two grasslands. Environ Pollut 103:239–250

    Article  Google Scholar 

  • Keeler BL, Hobbie SE, Kellogg LE (2009) Effects of long-term nitrogen addition on microbial enzyme activity in eight forested and grassland sites: implications for litter and soil organic matter decomposition. Ecosystems 12:1–15

    Article  CAS  Google Scholar 

  • Kepner RL, Pratt JT (1994) Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present. Microbiol Mol Biol R 58:603–615

    CAS  Google Scholar 

  • Kuzyakov Y (2010) Priming effects: interactions between living and dead organic matter. Soil Biol Biochem 42:1363–1371

    Article  CAS  Google Scholar 

  • Lal R, Kimble JM, Follett RF (1997) Pedospheric processes and the carbon cycle. In: Lal R, Kimble JM, Follett RF, Stewart BA (eds) Soil processes and the carbon cycle. CRC, New York, pp 1–8

    Google Scholar 

  • Leavitt SW, Follett RF, Paul EA (1996) Estimation of slow- and fast-cycling soil organic carbon pools from 6 N HCl hydrolysis. Radiocarbon 38:231–239

    CAS  Google Scholar 

  • Lin Z, Zhang R, Tang J, Zhang J (2010) Effects of high soil water content and temperature on soil respiration. Soil Sci 176:150–155

    Article  Google Scholar 

  • Lu X, Mo J, Gundersern P, Zhu W, Zhou G, Li D, Zhang X (2009) Effect of simulated N deposition on soil exchangeable cations in three forest types of subtropical China. Pedosphere 19(2):189–198

    Article  CAS  Google Scholar 

  • Magill AH, Aber JD, Hendricks JJ, Bowden RD, Melillo JM, Steudler PA (1997) Biogeochemical response of forest ecosystems to simulated chronic nitrogen deposition. Ecol Appl 7(2):402–447

    Article  Google Scholar 

  • Micks P, Aber JD, Boone RD, Davidson EA (2004) Short-term soil respiration and nitrogen immobilization response to nitrogen applications in control and nitrogen-enriched temperate forests. Forest Ecol Manag 196(1):57–70

    Article  Google Scholar 

  • Paul EA, Morris SJ, Conant RT, Plante AF (2006) Does the acid hydrolysis-incubation method measure meaningful soil organic carbon pools? Soil Sci Soc Am J 70:1023–1035

    Article  CAS  Google Scholar 

  • Personeni E, Luscher A, Loiseau P (2005) Rhizosphere activity, grass species and N availability effects on the soil C and N cycles. Soil Biol Biochem 37(5):819–827

    Article  CAS  Google Scholar 

  • Pietri JCA, Brookes PC (2009) Substrate inputs and pH as factors controlling microbial biomass, activity and community structure in an arable soil. Soil Biol Biochem 41:1396–1405

    Article  Google Scholar 

  • Rousk J, Brookes PC, Bååth E (2009) Contrasting soil pH effects on fungal and bacterial growth suggest functional redundancy in carbon mineralization. Appl Environ Microb 75:1589–1596

    Article  CAS  Google Scholar 

  • Rovira P, Vallejo VR (2002) Labile and recalcitrant pools of carbon and nitrogen in organic matter decomposing at different depths in soil: an acid hydrolysis approach. Geoderma 107:109–141

    Article  CAS  Google Scholar 

  • Saiya-Cork KR, Sinsabaugh RL, Zak DR (2002) The effects of long term N deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biol Biochem 34:1309–1315

    Article  CAS  Google Scholar 

  • Sinsabaugh RL, Klug MJ, Collins HP, Yeager PE, Petersen SO (1999) Characterizing soil microbial communities. In: Robertson GP, Coleman DC, Bledsoe CS, Sollins P (eds) Standard soil methods for long-term ecological research. Oxford University Press, New York, pp 318–348

    Google Scholar 

  • Sjöberg G, Knicker H, Nilsson SI, Berggren D (2004) Impact of long-term N fertilization on the structural composition of spruce litter and mor humus. Soil Biol Biochem 36:609–618

    Article  Google Scholar 

  • Snajdr J, Valaskova V, Merhautova V, Herinkova J, Cajthaml T, Baldrian P (2008) Spatial variability of enzyme activities and microbial biomass in the upper layers of Quercus petraea forest soil. Soil Biol Biochem 40:2068–2075

    Article  CAS  Google Scholar 

  • Söderström BE (1977) Vital staining of fungi in pure cultures and in soil with fluorescein diacetate. Soil Biol Biochem 9:59–63

    Article  Google Scholar 

  • Trumbore SE, Bonani G, Wolfli W (1990) The rates of carbon cycling in several soils from AMS 14C measurements of fractionated soil organic matter. In: Bouwman AF (ed) Soils and the greenhouse effect. Wiley, New York, pp 405–414

    Google Scholar 

  • Waldrop MP, Zak DR, Sinsabaugh RL (2004) Microbial community response to nitrogen deposition in northern forest ecosystems. Soil Biol Biochem 36:1443–1451

    Article  CAS  Google Scholar 

  • Zhang N, Wan S, Li L, Bi J, Zhao M, Ma K (2008) Impacts of urea N addition on soil microbial community in a semi-arid temperate steppe in northern China. Plant Soil 311:19–28

    Article  CAS  Google Scholar 

  • Zheng X, Fu C, Xu X, Xiao D, Huang Y, Chen G, Han S, Hu F (2002) The Asian nitrogen cycle case study. Ambio 31:79–87

    Google Scholar 

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Acknowledgments

This work was partly supported by grants from the Chinese National Natural Science Foundation (Nos. 51039007 and 51179212) and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou, 510275, People’s Republic of China

    Xinyu Jiang & Renduo Zhang

  2. School of Life Science, Sun Yat-sen University, Guangzhou, 510275, China

    Lixiang Cao

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  1. Xinyu Jiang
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  2. Lixiang Cao
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Correspondence to Renduo Zhang.

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Responsible editor: Hailong Wang

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Jiang, X., Cao, L. & Zhang, R. Changes of labile and recalcitrant carbon pools under nitrogen addition in a city lawn soil. J Soils Sediments 14, 515–524 (2014). https://doi.org/10.1007/s11368-013-0822-z

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