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In situ ATR-FTIR study on the adhesion of Pseudomonas putida to Red soil colloids

  • SOILS, SEC 2 • GLOBAL CHANGE, ENVIRON RISK ASSESS, SUSTAINABLE LAND USE • RESEARCH ARTICLE
  • Published:
Journal of Soils and Sediments Aims and scope Submit manuscript

Abstract

Purpose

Bacterial adhesion to soil particles is fundamentally important in mineral weathering, organic matter degradation, heavy metal transformation, and fate of pollutants. However, the adhesion mechanism between bacteria and soil colloids under continuous flow systems in the natural environments remains unknown.

Materials and methods

The kinetics of Pseudomonas putida cellular adsorption and desorption on Red soil colloid films under controlled flow systems were examined using in situ attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Derjaguin–Landau–Verwey–Overbeek (DLVO) and non-DLVO interactions were employed to elucidate the cellular adsorption and desorption kinetics.

Results and discussion

In situ ATR-FTIR spectroscopy can be used effectively to investigate the kinetics of bacterial adhesion to a soil colloid deposit. Surface proteins may be involved in the bacterial adhesion to soil colloids. The adsorption followed pseudo-first-order kinetic equation. High adsorption rate constant and great saturation coverage of adsorbed bacteria were found at high ionic strengths in dynamic systems.

Conclusions

P. putida bacterial cellular adsorption on the soil colloid deposit was irreversible in a wide range of ionic strengths under controlled flow systems. The less reversible adhesion was probably attributed to the DLVO predicted deep secondary energy minima together with non-DLVO factors including polymer bridging, local charge heterogeneities, surface roughness, and Lewis acid–base interactions.

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Acknowledgments

This work was kindly funded by the National Natural Science Foundation of China (40825002) and the Fundamental Research Funds for the Central Universities (2012YB17).

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China

    Huayong Wu, Xingmin Rong, Peng Cai, Ke Dai & Qiaoyun Huang

  2. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China

    Wenli Chen

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  1. Huayong Wu
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  2. Wenli Chen
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  3. Xingmin Rong
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  4. Peng Cai
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Correspondence to Qiaoyun Huang.

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Responsible editor: Hailong Wang

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Wu, H., Chen, W., Rong, X. et al. In situ ATR-FTIR study on the adhesion of Pseudomonas putida to Red soil colloids. J Soils Sediments 14, 504–514 (2014). https://doi.org/10.1007/s11368-013-0817-9

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  • DOI: https://doi.org/10.1007/s11368-013-0817-9

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