Abstract
Purpose
Marine environments, especially sediments, are rich sources of actinomycetes that provide many bioactive compounds, primarily antibiotics. The goal of this study was to investigate the diversity of cultivable actinomycetes and their potential to produce antibiotics from sediments collected from the coastal zones of Turkey.
Materials and methods
Thirty sediment samples were collected from nine different coastal sites in three seas surrounding the Anatolian Peninsula of Turkey. Of the samples, 6 were collected from one site in the Black Sea, 18 from seven sites in the Aegean Sea, and 6 from one site in the Mediterranean Sea. Strains of pure actinomycetes were isolated by modified actinomycetes isolation agar (MAIA), M1 agar, M6 agar, and modified R2A agar. Ethyl acetate extracts and fermentation broths were used for the evaluation of antimicrobial activity against antibiotic resistant test microorganisms. The identification of the isolates was undertaken by 16S rRNA gene sequencing.
Results and discussion
A total of 261 strains of actinomycetes were isolated, of which 66 (25 %) were active against at least one antibiotic-resistant microorganism. Sixty-five of the actinomycetes isolates with antimicrobial activity were Streptomyces spp. and one was Nocardia sp., which implied that genus Streptomyces was predominant. Whereas MAIA agar was the best medium to recover actinomycetes, M6 agar was superior to others for the isolation of antibiotic-producing strains.
Conclusions
Extensive screening of the extracts from the 261 isolates for antimicrobial activities revealed considerable potential to produce antibiotics. These findings imply that actinomycetes from marine sediments of the Anatolian Peninsula coasts have potential for the discovery of novel bioactive compounds.
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Acknowledgments
This study was supported financially by the Scientific and Technological Research Council of Turkey (TÜBİTAK, SBAG-109S361) and Ege University Research Foundation (2009 Fen 061). We would also like to thank Associate Professor İkbal Agah Ince for his valuable help with the sequences and M. Baki Yokeş for providing the sediment samples.
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Özcan, K., Aksoy, S.Ç., Kalkan, O. et al. Diversity and antibiotic-producing potential of cultivable marine-derived actinomycetes from coastal sediments of Turkey. J Soils Sediments 13, 1493–1501 (2013). https://doi.org/10.1007/s11368-013-0734-y
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DOI: https://doi.org/10.1007/s11368-013-0734-y