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Journal of Soils and Sediments

, Volume 10, Issue 3, pp 389–399 | Cite as

Integrating the fish embryo toxicity test as triad element for sediment toxicity assessment based on the Water Framework Directive approach

  • Mariana BartzkeEmail author
  • Vera Delov
  • Petra Stahlschmidt-Allner
  • Bernhard Allner
  • Jörg Oehlmann
SEDIMENTS, SEC 2 • SEDIMENT RISK MANAGEMENT AND COMMUNICATION • RESEARCH ARTICLE

Abstract

Purpose

The objective of this study was to complement analyses according to the European Union Water Framework Directive (WFD) with a sediment toxicity analysis as part of an integrated river assessment. To this end, Hessian water courses were analyzed using the sediment quality triad concept according to Chapman with chemical analyses, in situ effect evaluations, and ecotoxicological assessments. For the ecotoxicological assessment (fish embryo toxicity test with Danio rerio), a new evaluation scheme was developed, the fish teratogenicity index (FTI), that allows for a classification of sediments into ecological quality classes compliant to the WFD.

Materials and methods

Sediment and macrozoobenthos samples were taken from tributaries of the rivers Fulda and Lahn. Sediments were characterized regarding particle size, carbon, heavy metals, and polyaromatic hydrocarbon content. Macroinvertebrate samples were taken via multi-habitat sampling. The fish embryo toxicity test with D. rerio was conducted as a contact assay on the basis of DIN 38415-6.

Results and discussion

The integrated assessment indicated a significant influence of heavy metals and carbon content on macroinvertebrate communities. The bioaccessibility of sediment pollutants were clearly demonstrated by the FTI, which showed a wide range of adverse effects. A significant linear relationship between metals and the FTI was detected. However, there was no statistically significant evidence that macroinvertebrate communities were affected by the hydromorphological quality elements at the sampling sites.

Conclusions

The new scheme for the assessment of fish embryo toxicity test was successfully applied. The results suggest that sediment compounds impact macroinvertebrate communities and early development of fish. It demonstrates that the quality of sediments should be evaluated on a routine basis as part of an integrated river assessment.

Keywords

Bioassay Danio rerio Fish teratogenicity index Macroinvertebrates Metals Sediment toxicity 

Notes

Acknowledgements

The authors are particularly grateful for the support of sediment analysis by Heike Heidenreich and Gerlinde Liepelt from the International Graduate School Zittau as well as for collaborative support by Simone Galluba and Maren Heß from Goethe University Frankfurt am Main and Dietmar Bernauer. Moreover, the authors wish to thank Mirco Weil from ECT Oekotoxikologie GmbH for support with ammonia toxicity testing.

Supplementary material

11368_2009_170_MOESM1_ESM.pdf (330 kb)
Supplement 1 (PDF 330 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Mariana Bartzke
    • 1
    • 2
    • 3
    Email author
  • Vera Delov
    • 1
    • 2
    • 4
  • Petra Stahlschmidt-Allner
    • 2
  • Bernhard Allner
    • 2
  • Jörg Oehlmann
    • 1
  1. 1.Department Aquatic EcotoxicologyGoethe University Frankfurt am MainFrankfurtGermany
  2. 2.Gobio GmbHAarbergen/KettenbachGermany
  3. 3.Department Bioanalytical EcotoxicologyHelmholtz Centre for Environmental Research, UFZLeipzigGermany
  4. 4.EcotoxicologyFraunhofer Institute for Molecular Biology and Applied Ecology IMEAachenGermany

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