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Impact of 14-day bed rest on serum adipokines and low-grade inflammation in younger and older adults

Abstract

Ageing and inactivity both contribute to systemic inflammation, but the effects of inactivity on inflammation in healthy elderly individuals have not been elucidated. We hypothesised that 14-day bed rest could affect the pro- and anti-inflammatory markers in young subjects differently than in older adults. A short-term 14-day horizontal bed rest study (BR14) has been used as a model of inactivity in two groups of healthy male volunteers: 7 aged 18–30 years (young) and 16 aged 55–65 years (older adults). The effects of inactivity on inflammation were compared. Key low-grade inflammation mediators, tumour necrosis factor α (TNF-α), interleukin-6 (IL-6), visfatin, resistin, and anti-inflammatory adiponectin were measured in fasting serum samples, collected at baseline (BDC) and post BR14. Young responded to BR14 by increasing serum visfatin and resistin while older adults responded to BR14 by increasing IL-6 and TNF-α. In addition, serum adiponectin increased in all participants. Data from correlation analysis demonstrated positive association between Δ serum visfatin and Δ IL-6 in both groups, while Δ serum adiponectin was negatively associated with Δ TNF-α in young and positively associated with Δ resistin in the older adults. As little as 14 days of complete physical inactivity (BR14) negatively affected markers of low-grade inflammation in both groups, but the inflammation after BR14 was more pronounced in older adults. The effect of BR14 on IL-6 and resistin differed between young and older adults. Inflammatory responses to BR14 in older adults differed from those reported in the literature for obese or subjects in pathological states, suggesting potentially different mechanisms between inactivity- and obesity-induced inflammations.

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Acknowledgments

We would like to thank the participants for their time and effort to ensure the success of this study. We acknowledge the excellent assistance of the entire staff of the Valdoltra Orthopaedic Hospital of Ankaran (Koper, Slovenia). Additionally, we thank the research team and the students of Applied Kinesiology of University of Primorska for the help and logistic support and many others who contributed and took care that the study ran smoothly. Special thanks go to Vanja Pahor from Izola General Hospital biochemical laboratory for the support in biochemical analysis. The study was conducted by Slovenian and Italian partners included in the standard project PANGeA: Physical Activity and Nutrition for Quality Ageing which was supported by grant from Cross-border Cooperation Programme Slovenia–Italy 2007-2013 and co-financed by European Regional Development Fund and Slovenian and Italian national funds.

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Correspondence to Mihaela Jurdana.

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Mihaela Jurdana, Zala Jenko-Pražnikar and Nina Mohorko equally contributed to this work.

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Jurdana, M., Jenko-Pražnikar, Z., Mohorko, N. et al. Impact of 14-day bed rest on serum adipokines and low-grade inflammation in younger and older adults. AGE 37, 116 (2015). https://doi.org/10.1007/s11357-015-9848-z

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  • DOI: https://doi.org/10.1007/s11357-015-9848-z

Keywords

  • Complete inactivity
  • Inflammation
  • Adiponectin
  • TNF-α
  • IL-6