A botanical containing freeze dried açai pulp promotes healthy aging and reduces oxidative damage in sod1 knockdown flies
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Superoxide dismutase 1 (SOD1), a critical enzyme against oxidative stress, is implicated in aging and degenerative diseases. We previously showed that a nutraceutical containing freeze-dried açai pulp promotes survival of flies fed a high-fat diet or sod1 knockdown flies fed a standard diet. Here, we investigated the effect of açai supplementation initiated at the early or late young adulthood on lifespan, physiological function, and oxidative damage in sod1 knockdown flies. We found that Açai supplementation extended lifespan even when started at the age of 10 days, which is the time shortly before the mortality rate of flies accelerated. Life-long açai supplementation increased lifetime reproductive output in sod1 knockdown flies. Our molecular studies indicate that açai supplementation reduced the protein levels of genes involved in oxidative stress response, cellular growth, and nutrient metabolism. Açai supplementation also affected the protein levels of ribosomal proteins. In addition, açai supplementation decreased the transcript levels of genes involved in oxidative stress response and gluconeogenesis, while increasing the transcript levels of mitochondrial biogenesis genes. Moreover, açai supplementation reduced the level of 4-hydroxynonenal-protein adducts, a lipid peroxidation marker. Our findings suggest that açai supplementation promotes healthy aging in sod1-deficient flies partly through reducing oxidative damage, and modulating nutrient metabolism and oxidative stress response pathways. Our findings provide a foundation to further evaluate the viability of using açai as an effective dietary intervention to promote healthy aging and alleviate symptoms of diseases with a high level of oxidative stress.
KeywordsAging intervention Açai Superoxide dismutase 1 Lifespan Oxidative stress Reproductive aging Drosophila melanogaster
We thank Alex Schauss to provide us the açai pulp extract and thank Ed Spangler, Alex Schauss, Don Ingram, Jim Carey, and Pablo Liedo for critical reading of the manuscript. This work was supported by funding from the Intramural Research Program of the National Institute on Aging, NIH to S.Z. and a research grant from the NIH Office of Dietary Supplements to X.P. and S.Z.
- Ang ET, Tai YK, Lo SQ, Seet R, Soong TW (2010) Neurodegenerative diseases: exercising toward neurogenesis and neuroregeneration. Front Ag Neurosci 2:25Google Scholar
- Ashburner M (1989) Drosophila: A laboratory handbook. Cold Spring Harbor Laboratory Press, PlainviewGoogle Scholar
- Baur JA, Pearson KJ, Price NL, Jamieson HA, Lerin C, Kalra A, Prabhu VV, Allard JS, Lopez-Lluch G, Lewis K, Pistell PJ, Poosala S, Becker KG, Boss O, Gwinn D, Wang M, Ramaswamy S, Fishbein KW, Spencer RG, Lakatta EG, Le Couteur D, Shaw RJ, Navas P, Puigserver P, Ingram DK, de Cabo R, Sinclair DA (2006) Resveratrol improves health and survival of mice on a high-calorie diet. Nature 444(7117):337–342PubMedCrossRefGoogle Scholar
- Civitarese AE, Smith SR, Ravussin E (2007) Diet, energy metabolism and mitochondrial biogenesis. Curr Opin Clin Nutr Metab Care 10(6):679–687. doi: 10.1097/MCO.0b013e3282f0ecd2.00075197-200711000-00005 PubMedCrossRefGoogle Scholar
- Feio CA, Izar MC, Ihara SS, Kasmas SH, Martins CM, Feio MN, Maues LA, Borges NC, Moreno RA, Povoa RM, Fonseca FA (2011) Euterpe Oleracea (Acai) Modifies Sterol Metabolism and Attenuates Experimentally-Induced Atherosclerosis. J Atherosclerosis ThrombGoogle Scholar
- Heli H, Mirtorabi S, Karimian K (2011) Advances in iron chelation: an update. Expert Rev Neurother 21(6):819–856Google Scholar
- Poulose SM, Fisher DR, Larson JA, Bielinski DF, Rimando AM, Carey AN, Schauss AG, Shukitt-Hale B (2012) Anthocyanin-rich acai (Euterpe oleracea Mart.) fruit pulp fractions attenuate inflammatory stress signaling in mouse brain BV-2 microglial cells. J Agric Food Chem 60(4):1084–1093. doi: 10.1021/jf203989k PubMedCrossRefGoogle Scholar
- Schauss AG, Wu X, Prior RL, Ou B, Huang D, Owens J, Agarwal A, Jensen GS, Hart AN, Shanbrom E (2006a) Antioxidant capacity and other bioactivities of the freeze-dried Amazonian palm berry, Euterpe oleraceae Mart. (acai). J Agric Food Chem 54(22):8604–8610. doi: 10.1021/jf0609779 PubMedCrossRefGoogle Scholar
- Srivastava RA (2009) Fenofibrate ameliorates diabetic and dyslipidemic profiles in KKAy mice partly via down-regulation of 11beta-HSD1, PEPCK and DGAT2. Comparison of PPARalpha, PPARgamma, and liver x receptor agonists. Eur J Pharmacol 607(1–3):258–263. doi: 10.1016/j.ejphar.2009.02.024 PubMedCrossRefGoogle Scholar
- Xie C, Kang J, Li Z, Schauss AG, Badger TM, Nagarajan S, Wu T, Wu X (2012) The acai flavonoid velutin is a potent anti-inflammatory agent: blockade of LPS-mediated TNF-alpha and IL-6 production through inhibiting NF-kappaB activation and MAPK pathway. J Nutr Biochem (in press)Google Scholar
- Zou S, Carey JR, Liedo P, Ingram DK, Yu B (2011) Prolongevity effects of a botanical with oregano and cranberry extracts in Mexican fruit flies: examining interactions of diet restriction and age. Age (Dordrecht, Netherlands) 34(2):269-279Google Scholar