Calorie restriction (CR) is a reliable anti-aging intervention that attenuates the onset of a number of age-related diseases, reduces oxidative damage, and maintains function during aging. In the current study, we assessed the effects of CR and other feeding regimens on wound healing in 7-month-old Fischer-344 rats from a larger cohort of rats that had been fed either ad libitum (AL) or 40% calorie restricted based on AL consumption. Rats were assigned to one of three diet groups that received three skin punch wounds along the dorsal interscapular region (12-mm diameter near the front limbs) of the back as follows: (1) CR (n = 8) were wounded and maintained on CR until they healed, (2) AL (n = 5) were wounded and maintained on AL until wound closure was completed, and (3) CR rats were refed (RF, n = 9) AL for 48 h prior to wounding and maintained on AL until they healed. We observed that young rats on CR healed more slowly while CR rats refed for 48 h prior to wounding healed as fast as AL fed rats, similar to a study reported in aged CR and RF mice (Reed et al. 1996). Our data suggest that CR subjects, regardless of age, fail to heal well and that provision of increased nutrition to CR subjects prior to wounding enhances the healing process.
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Peroxisome proliferator-activated receptor gamma coactivator-1alpha
Silent information regulator 1
Peroxisome proliferator-activated receptor (α—alpha, β/Δ—beta/delta, γ—gamma)
Ayello EA, Thomas DR, Litchford MA (1999) Nutritional aspects of wound healing. Home Health Nurse 17:719–729
Ballas CB, Davidson JM (2001) Delayed wound healing in aged rats is associated with increased collagen gel remodeling and contraction by skin fibroblasts, not with differences in apoptotic or myofibroblast cell populations. Wound Repair Regen 9(3):223–237
Chipalkatti S, De AK, Alyar AS (1983) Effect of diet restriction on some biochemical parameters related to aging in mice. J Nutr 113:944–950
de Luca C, Deeva I, Mikhal’Chik E, Korkina L (2007) Beneficial effects of pro-/antioxidant-based nutraceuticals in the skin rejuvenation techniques. Cell Mol Biol 53:94–101 (Noisy-le-grand)
Lim Y, Levy MA, Bray TM (2006) Dietary supplementation of N-acetylcysteine enhances early inflammatory responses during cutaneous wound healing in protein malnourished mice. J Nutr Biochem 17:328–336
Masoro EJ (1988) Life span extension and food restriction. Compr Ther 14(6):9–13
Reed MJ, Penn PE, Li Y, Birnbaum R, Vernon RB, Johnson TS, Pendergrass WR, Sage EH, Abrass IB, Wolf NS (1996) Enhanced cell proliferation and biosynthesis mediate improved wound repair in refed, caloric-restricted mice. Mech Ageing Dev 89(1):21–43
Reiser K, McGee C, Rucker R, McDonald R (1995) Effects of aging and caloric restriction on extracellular matrix biosynthesis in a model of injury repair in rats. 50A(1): B40–B47
Roth GS, Kowatch MA, Hengemihle J, Ingram DK, Spangler EL, Johnson LK, Lane MA (1997) Effect of age and caloric restriction on cutaneous wound closure in rats and monkeys. J Gerontol A Biol Sci Med Sci 52(2):B98–B102
Weindruch R, Walford RL (1988) The retardation of aging and disease by dietary restriction. Thomas, Springfield
This research was supported (in part) by the Intramural Research Program of the NIH, National Institute on Aging.
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Hunt, N.D., Li, G.D., Zhu, M. et al. Effect of calorie restriction and refeeding on skin wound healing in the rat. AGE 34, 1453–1458 (2012). https://doi.org/10.1007/s11357-011-9321-6