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n-Butanol fraction of moringa seed attenuates arsenic intoxication by regulating the uterine inflammatory and apoptotic pathways

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Abstract

The adverse effects of arsenic-chelating drugs make it essential to replace invasive chelating therapy with non-invasive oral therapy for arsenic poisoning. The goal of the current investigation was to determine whether the uterine damage caused by arsenization could be repaired by the n-butanol fraction of Moringa oleifera seed (NB). The rats were orally administered with arsenic (10 mg/kg BW) for the initial 8 days, followed by NB (50 mg/kg) for the next 8 days without arsenic. The probable existence of different components in NB was evaluated by HPLC–MS. Pro and anti-inflammatory indicators were assessed by RT-PCR and western blot. ESR-α was detected via immunostaining. Arsenic-exposed rats had significantly increased lipid peroxidation and decreased antioxidant enzyme activity, which were markedly reduced after NB treatment. Weaker ESR-α expression and distorted uterine histomorphology following arsenication were retrieved significantly by NB. Meaningful restoration by NB was also achieved for altered mRNA and protein expression of various inflammatory and apoptotic indicators. Molecular interaction predicted that glucomoringin and methyl glucosinolate of moringa interact with the catalytic site of caspase-3 in a way that limits its activity. However, NB was successful in restoring the arsenic-mediated uterine hypofunction. The glucomoringin and methyl glucosinolate present in n-butanol fraction may play a critical role in limiting apoptotic event in the arsenicated uterus.

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Acknowledgements

We gratefully acknowledge Prof. Sathees C. Raghavan of the Department of Biochemistry at the Indian Institute of Science in Bangalore, India, for providing us with technical support and lab space.

Funding

The Department of Science and Technology, Government of West Bengal, provided funding for the study under sanction 1212 (sanc.)/ST/P/S&T/1G-13/2015.

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Authors and Affiliations

  1. Department of Biomedical Laboratory Science and Management and Clinical Nutrition and Dietetics, Vidyasagar University, Midnapore, 721102, West Bengal, India

    Suryashis Jana, Angshita Ghosh, Arindam Dey, Hasina Perveen, Shilpa Maji & Sandip Chattopadhyay

  2. Department of Medical Laboratory Technology (MLT), Haldia Institute of Health Sciences, ICARE Complex, Hatiberia, Purba Medinipur, 721657, West Bengal, India

    Pikash Pratim Maity

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  1. Suryashis Jana
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Contributions

Animal treatment, biochemical test, histology, and immunostaining were performed by Suryashis Jana. Western blot analysis was performed by Hasina Perveen. Native gel electrophoresis and PCR were performed by Arindam Dey and Shilpa Maji. Molecular docking and manuscript editing were performed by Angshita Ghosh. Experimental protocol design and manuscript writing were performed by Sandip Chattopadhyay. Statistical analysis and manuscript editing were done by Pikash Pratim Maity.

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Correspondence to Sandip Chattopadhyay.

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Ethical clearance number IEC/7–4/C-4/16. Vidyasagar University Animal Ethical Committee constituted by CPCSEA, MOEF&CC, Govt. of India, approved this experiment.

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Jana, S., Ghosh, A., Dey, A. et al. n-Butanol fraction of moringa seed attenuates arsenic intoxication by regulating the uterine inflammatory and apoptotic pathways. Environ Sci Pollut Res 31, 18593–18613 (2024). https://doi.org/10.1007/s11356-024-32213-4

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