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Interference mechanism of benzo[a]pyrene exposure on the taste substance metabolisms in Ruditapes philippinarum

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Abstract

Aquatic animals are popular for their unique umami and high-quality protein. However, under the realistic background of increasing marine pollution, whether it affects the aquatic animal tastes, and what the interference mechanism is still remains unknown. Benzo[a]pyrene (B[a]P) is a typical Polycyclic aromatic hydrocarbons (PAHs) with high toxicity. In this study, we investigated the effects of B[a]P (0, 0.8, 4 and 20 μg/L) on the content and taste evaluation of Ruditapes philippinarum taste substances, and clarified the interference mechanism of B[a]P on taste substance metabolisms with transcriptome analysis. The results demonstrated that B[a]P significantly altered the contents and taste activity values (TAVs) of free amino acids (FAAs), 5′-nucleotides, organic acids, flavor peptides, organic bases, sugars and inorganic ions, as well as the gene expressions within their synthesis and decomposition, indicating that B[a]P affected these taste substance contents by interfering with their metabolisms, thereby changing the clam tastes (decreases of umami and sweetness, and increase of bitter taste). This study provided scientific basis for quality assurance of bivalve cultivation and control of marine pollution.

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Funding

This work was supported by the National Natural Science Foundation of China (32273106).

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Authors and Affiliations

  1. The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Yushan Road 5, Qingdao, 266003, People’s Republic of China

    Yaqi Bi, Aimin Song, Luqing Pan, Jingjing Miao, Yueyao Zhou & Zeyuan Li

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  1. Yaqi Bi
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  2. Aimin Song
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  3. Luqing Pan
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  4. Jingjing Miao
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  5. Yueyao Zhou
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  6. Zeyuan Li
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Contributions

Yaqi Bi: Conceptualization, Methodology, Investigation, Formal analysis, Resources, Writing—original draft. Aimin Song: Conceptualization, Methodology, Resources. Luqing Pan: Conceptualization, Resources. Jingjing Miao: Conceptualization, Resources, Writing—review & editing. Yueyao Zhou: Conceptualization, Writing—review & editing. Zeyuan Li: Investigation.

Corresponding author

Correspondence to Luqing Pan.

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All experimental procedures were conducted in conformity with institutional guidelines for the care and use of laboratory animals, and protocols were approved by the Institutional Animal Care and Use Committee in Ocean University of China, Qingdao, China.

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Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.

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The authors declare no competing interests.

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Highlights

• Firstly analyzed the effects of POPs on change features of bivalve taste substances.

• Firstly clarified the impact mechanism of POPs on bivalve taste substance metabolism.

• Affirmed the impact difference of POPs on taste substances in different sex bivalves.

Supplementary information

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Bi, Y., Song, A., Pan, L. et al. Interference mechanism of benzo[a]pyrene exposure on the taste substance metabolisms in Ruditapes philippinarum. Environ Sci Pollut Res 31, 12019–12035 (2024). https://doi.org/10.1007/s11356-024-31906-0

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  • DOI: https://doi.org/10.1007/s11356-024-31906-0

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