Abstract
A heterotrophic nitrifying aerobic denitrifying (HN-AD) strain ZQ-A1 with excellent denitrification performance, identified as Acinetobacter, was isolated from simultaneous nitrification and denitrification (SND) craft. ZQ-A1 was capable of removing NH4+, NO2–, and NO3–; the 21-hour removal rates were 84.84%, 87.13%, and 92.63%. ZQ-A1 has the ability to treat mixed nitrogen sources. In addition, ZQ-A1 can be well applied to actual sewage. According to the analysis of microbial community characteristics, the relative abundance of Acinetobacter in the experimental group increased from 0.06% to 2.38%, which is an important reason for the removal rate of NH4+ exceeding 99% within 30 days. The results of KEGG function prediction showed that with the addition of ZQ-A1, the relative abundance of pathways related to bacterial metabolism, such as tricarboxylic acid cycle metabolism, was higher. The research expanded the thinking of HN-AD bacteria in actual production and laid a foundation for its application in sewage treatment.
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Data availability
The data that support the findings of this study are available from the corresponding author upon reasonable request. The data presented in this study are available upon request from the corresponding author.
References
APHA (2005) Standard methods for the examination of water and wastewater. American Public Health Association (APHA), Washington, DC
Bru D, Sarr A, Philippot L (2007) Relative abundances of proteobacterial membrane-bound and periplasmic nitrate reductases in selected environments. Appl Environ Microbiol 73(18):5971–5974. https://doi.org/10.1128/AEM.00643-07
Chen J, Xu J, Zhang S et al (2021a) Nitrogen removal characteristics of a novel heterotrophic nitrification and aerobic denitrification bacteria, Alcaligenes faecalis strain WT14. J Environ Manage 282:111961. https://doi.org/10.1016/j.jenvman.2021.111961
Chen P, Zhang F, Zhang L et al (2022) Characterization of a novel salt-tolerant strain Sphingopyxis sp. CY-10 capable of heterotrophic nitrification and aerobic denitrification. Bioresour Technol 358:127353. https://doi.org/10.1016/j.biortech.2022.127353
Chen R, Deng M, He X et al (2017) Enhancing nitrate removal from freshwater pond by regulating carbon/nitrogen ratio. Front Microbiol 8:1712. https://doi.org/10.3389/fmicb.2017.01712
Chen X, Zhang Q, Zhu Y et al (2021b) Response of wastewater treatment performance, microbial composition and functional genes to different C/N ratios and carrier types in MBBR inoculated with heterotrophic nitrification-aerobic denitrification bacteria. Bioresour Technol 336:125339. https://doi.org/10.1016/j.biortech.2021.125339
Cui Y, Cui YW, Huang JL (2021) A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification. Bioresour Technol 333:125189. https://doi.org/10.1016/j.biortech.2021.125189
Deng M, Zhao X, Senbati Y et al (2021) Nitrogen removal by heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas sp. DM02: removal performance, mechanism and immobilized application for real aquaculture wastewater treatment. Bioresour Technol 322:124555. https://doi.org/10.1016/j.biortech.2020.124555
Duan H, Gao S, Li X et al (2020) Improving wastewater management using free nitrous acid (FNA). Water Res 171:115382. https://doi.org/10.1016/j.watres.2019.115382
Feng L, Jia R, Zeng Z et al (2018) Simultaneous nitrification-denitrification and microbial community profile in an oxygen-limiting intermittent aeration SBBR with biodegradable carriers. Biodegradation 29(5):473–486. https://doi.org/10.1007/s10532-018-9845-x
Gu X, Leng J, Zhu J et al (2022) Influence mechanism of C/N ratio on heterotrophic nitrification- aerobic denitrification process. Bioresour Technol 343:126116. https://doi.org/10.1016/j.biortech.2021.126116
He T, Li Z, Sun Q et al (2016) Heterotrophic nitrification and aerobic denitrification by Pseudomonas tolaasii Y-11 without nitrite accumulation during nitrogen conversion. Bioresour Technol 200:493–499. https://doi.org/10.1016/j.biortech.2015.10.064
He T, Zhang M, Ding C et al (2022) New insight into the nitrogen removal capacity and mechanism of Streptomyces mediolani EM-B2. Bioresour Technol 348:126819. https://doi.org/10.1016/j.biortech.2022.126819
Henry S, Bru D, Stres B et al (2006) Quantitative detection of the nosZ gene, encoding nitrous oxide reductase, and comparison of the abundances of 16S rRNA, narG, nirK, and nosZ genes in soils. Appl Environ Microbiol 72(8):5181–5189. https://doi.org/10.1128/AEM.00231-06
Holmes DE, Dang Y, Smith JA (2019) Nitrogen cycling during wastewater treatment. Adv Appl Microbiol 106:113–192. https://doi.org/10.1016/bs.aambs.2018.10.003
Hou P, Sun X, Fang Z et al (2021) Simultaneous removal of phosphorous and nitrogen by ammonium assimilation and aerobic denitrification of novel phosphate-accumulating organism Pseudomonas chloritidismutans K14. Bioresour Technol 340:125621. https://doi.org/10.1016/j.biortech.2021.125621
Huan C, Yan Z, Sun J et al (2022) Nitrogen removal characteristics of efficient heterotrophic nitrification-aerobic denitrification bacterium and application in biological deodorization. Bioresour Technol 363:128007. https://doi.org/10.1016/j.biortech.2022.128007
Huang MQ, Cui YW, Huang JL et al (2022) A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal. Water Res 221:118823. https://doi.org/10.1016/j.watres.2022.118823
Kong QX, Wang XW, Jin M et al (2006) Development and application of a novel and effective screening method for aerobic denitrifying bacteria. FEMS Microbiol Lett 260(2):150–155. https://doi.org/10.1111/j.1574-6968.2006.00306.x
Kong Z, Li L, Wu J et al (2022) Unveiling the characterization and development of prokaryotic community during the start-up and long-term operation of a pilot-scale anaerobic membrane bioreactor for the treatment of real municipal wastewater. Sci Total Environ 813:152643. https://doi.org/10.1016/j.scitotenv.2021.152643
Kouba V, Svehla P, Catrysse M et al (2019) How biomass growth mode affects ammonium oxidation start-up and NOB inhibition in the partial nitritation of cold and diluted reject water. Environ Technol 40(6):673–682. https://doi.org/10.1080/09593330.2017.1403491
Lang X, Li Q, Xu Y et al (2019) Aerobic denitrifiers with petroleum metabolizing ability isolated from caprolactam sewage treatment pool. Bioresour Technol 290:121719. https://doi.org/10.1016/j.biortech.2019.121719
Li Y, Liang H, Yang W et al (2022) Enhanced nitrogen removal in mainstream deammonification systems at ambient temperature by novel modified carriers and differentiation of microbial community transformation. Bioresour Technol 366:128158. https://doi.org/10.1016/j.biortech.2022.128158
Liang Y, Wu C, Wei X et al (2021) Characterization of nirS- and nirK-containing communities and potential denitrification activity in paddy soil from eastern China. Agric Ecosyst Environ 107561. https://doi.org/10.1016/j.agee.2021.107561
Lin JT, Stewart V (1998) Nitrate assimilation by bacteria. Adv Microb Physiol 39:1–379. https://doi.org/10.1016/s0065-2911(08)60014-4
Liu B, Mao Y, Bergaust L et al (2013) Strains in the genus Thauera exhibit remarkably different denitrification regulatory phenotypes[J]. Environ Microbiol 15(10):2816–2828
Liu S, Liu Q, Wu H et al (2022) Integrative chemical and omics analysis of the ammonia nitrogen removal characteristics and mechanism of a novel oligotrophic heterotrophic nitrification-aerobic denitrification bacterium. Sci Total Environ 852:158519. https://doi.org/10.1016/j.scitotenv.2022.158519
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 25(4):402–408. https://doi.org/10.1006/meth.2001.1262
Ren J, Bai X, Liu Y et al (2021) Simultaneous nitrification and aerobic denitrification by a novel isolated Ochrobactrum anthropi HND19. Bioresour Technol 340:125582. https://doi.org/10.1016/j.biortech.2021.125582
Richardson DJ, Wehrfritz JM, Keech A et al (1998) The diversity of redox proteins involved in bacterial heterotrophic nitrification and aerobic denitrification. Biochem Soc Trans 26(3):401–408. https://doi.org/10.1042/bst0260401
Robertson LA, Kuenen JG (1990) Combined heterotrophic nitrification and aerobic denitrification in Thiosphaera pantotropha and other bacteria. Antonie Van Leeuwenhoek 57(3):139–152. https://doi.org/10.1007/BF00403948
Romani M, Hofer DC, Katsyuba E et al (2019) Niacin: an old lipid drug in a new NAD+ dress. J Lipid Res 60(4):741–746. https://doi.org/10.1194/jlr.S092007
Sun B, Cole JR, Tiedje JM (2001) Desulfomonile limimaris sp. nov., an anaerobic dehalogenating bacterium from marine sediments. Int J Syst Evol Microbiol 51(Pt 2):365–371. https://doi.org/10.1099/00207713-51-2-365
Wu L, Ding X, Lin Y et al (2022) Nitrogen removal by a novel heterotrophic nitrification and aerobic denitrification bacterium Acinetobacter calcoaceticus TY1 under low temperatures. Bioresour Technol 353:127148. https://doi.org/10.1016/j.biortech.2022.127148
Xi H, Zhou X, Arslan M et al (2022) Heterotrophic nitrification and aerobic denitrification process: promising but a long way to go in the wastewater treatment. Sci Total Environ 805:150212. https://doi.org/10.1016/j.scitotenv.2021.150212
Zhang QL, Liu Y, Ai GM, Miao LL, Zheng HY, Liu ZP (2012) The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresour Technol 108:35–44
Zhang M, Pan L, Su C et al (2021) Simultaneous aerobic removal of phosphorus and nitrogen by a novel salt-tolerant phosphate-accumulating organism and the application potential in treatment of domestic sewage and aquaculture sewage. Sci Total Environ 758:143580. https://doi.org/10.1016/j.scitotenv.2020.143580
Zhang M, He T, Chen M et al (2022) Ammonium and hydroxylamine can be preferentially removed during simultaneous nitrification and denitrification by Pseudomonas taiwanensis EN-F2. Bioresour Technol 350:126912. https://doi.org/10.1016/j.biortech.2022.126912
Zhang Y, Shi Z, Chen M et al (2015) Evaluation of simultaneous nitrification and denitrification under controlled conditions by an aerobic denitrifier culture. Bioresour Technol 175:602–605. https://doi.org/10.1016/j.biortech.2014.10.016
Zhao B, He YL, Hughes J et al (2010) Heterotrophic nitrogen removal by a newly isolated Acinetobacter calcoaceticus HNR. Bioresour Technol 101(14):5194–5200. https://doi.org/10.1016/j.biortech.2010.02.043
Zhao B, An Q, He YL et al (2012) N2O and N2 production during heterotrophic nitrification by Alcaligenes faecalis strain NR. Bioresour Technol 116:379–385. https://doi.org/10.1016/j.biortech.2012.03.113
Zhao B, Tian M, An Q, Ye J, Guo JS (2017) Characteristics of a heterotrophic nitrogen removal bacterium and its potential application on treatment of ammonium-rich wastewater. Bioresour Technol 226:46–54. https://doi.org/10.1016/j.biortech.2016.11.120
Zheng HY, Liu Y, Gao XY et al (2012) Characterization of a marine origin aerobic nitrifying-denitrifying bacterium. J Biosci Bioeng 114(1):33–37. https://doi.org/10.1016/j.jbiosc.2012.02.025
Funding
This research was funded by the Guangdong Major Project of Basic and Applied Basic Research (No. 2020B0301030007) and the Natural Science Foundation of Guangdong Province (2022A1515012473; 2020A1515010295).
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Conceptualization, Z.C.; methodology, Z.C. and F.H.; formal analysis, Z.C. and F.H.; writing—original draft preparation, Z.C., X.Y.Z., and R.Y.Z.; writing—review and editing, Y.W., X.D.L., and Y.B.W.; supervision, M.J.Y., Y.Y.F., and L.T. All authors have read and agreed to the published version of the manuscript.
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Cao, Z., Huang, F., Zhang, R. et al. Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification bacterium Acinetobacter ZQ-A1 and community characteristics analysis of its application in pig farm wastewater. Environ Sci Pollut Res 30, 104029–104042 (2023). https://doi.org/10.1007/s11356-023-29556-9
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DOI: https://doi.org/10.1007/s11356-023-29556-9