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Influence of Different Arsenic Species on the Bioavailability and Bioaccumulation of Arsenic by Sargassum horneri C. Agardh: Effects under Different Phosphate Conditions

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Abstract

The growth response and incorporation of As into the Sargassum horneri was evaluated for up to 7 days using either arsenate (As(V)), arsenite (As(III)) or methylarsonate (MMAA(V) and DMAA(V)) at 0, 0.25, 0.5, 1, 2, and 4 μM with various phosphate (P) levels (0, 2.5, 5 and 10 μM). Except As(III), algal chlorophyll fluorescence was almost similar and insignificant, regardless of whether different concentrations of P or As(V) or MMAA(V) or DMAA(V) were provided (p > 0.05). As(III) at higher concentrations negatively affected algal growth rate, though concentrations of all As species had significant effects on growth rate (p < 0.01). Growth studies indicated that toxicity and sensitivity of As species to the algae followed the trend: As(III) > As(V) > MMAA(V) ~ DMAA(V). As bioaccumulation was varied significantly depending on the increasing concentrations of all As species and increasing P levels considerably affected As(V) uptake but no other As species uptake (p < 0.01). The algae accumulated As(V) and As(III) more efficiently than MMAA(V) and DMAA(V). At equal concentrations of As (4 μM) and P (0 μM), the alga was able to accumulate 638.2 ± 71.3, 404.1 ± 70.6, 176.7 ± 19.6, and 205.6 ± 33.2 nM g-1 dry weight of As from As(V), As(III), MMAA(V), and DMAA(V), respectively. The influence of low P levels with increased As(V) concentrations more steeply increased As uptake, but P on other As species did not display similar trends. The algae also showed passive modes for As adsorption of all As species. The maximum adsorption of As (63.7 ± 6.1 nM g-1 dry weight) was found due to 4 μM As(V) exposure, which was 2.5, 7.3, and 6.9 times higher than the adsorption amounts for the same concentration of As(III), MMAA(V), and DMAA(V) exposure, respectively. The bioavailability and accumulation behaviors of As were significantly influenced by P and As species, and this information is essential for As research on marine ecosystems.

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Data availability

The data that support the findings of this study are available on request from the corresponding author.

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Acknowledgements

The study has been partially supported by Grants-in-Aid for Scientific Research (18H03399 and 19K21897) from the Japan Society for the Promotion of Science.

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All authors participated in the conception and design of the study. M. Abdullah Al Mamun, Shuhei Hayashi, and Rimana Islam Papry prepared the materials, collected the data, and conducted the chemical analysis. M. Abdullah Al Mamun performed the statistical analysis and wrote the first version of the manuscript. Osamu Miki, Ismail M. M. Rahman, and Asami S. Mashio discussed the results. Hiroshi Hasegawa supervised the study. All authors commented on and reviewed initial drafts of the manuscript and approved the final manuscript.

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Correspondence to M. Abdullah Al Mamun.

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Mamun, M.A., Hayashi, S., Papry, R.I. et al. Influence of Different Arsenic Species on the Bioavailability and Bioaccumulation of Arsenic by Sargassum horneri C. Agardh: Effects under Different Phosphate Conditions. Environ Sci Pollut Res 30, 98246–98260 (2023). https://doi.org/10.1007/s11356-023-29371-2

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