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Metal-immobilizing Pseudomonas taiwanensis WRS8 reduces heavy metal accumulation in Coriandrum sativum by changing the metal immobilization–related bacterial population abundances

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Abstract

Metal-immobilizing bacteria play a critical role in metal accumulation in vegetables. However, little is known concerning the mechanisms involved in bacteria-induced reduced metal availability and uptake in vegetables. In this study, the impacts of metal-immobilizing Pseudomonas taiwanensis WRS8 on the plant biomass, Cd and Pb availability and uptake in two coriander (Coriandrum sativum L.) cultivars, and bacterial community structure were investigated in the polluted soil. Strain WRS8 increased the biomass of two coriander cultivars by 25–48% and reduced Cd and Pb contents in the edible tissues by 40–59% and available Cd and Pb contents in the rhizosphere soils by 11.1–15.2%, compared with the controls. Strain WRS8 significantly increased the pH values and relative abundances of the dominant populations of Sphingomonas, Pseudomonas, Gaiellales, Streptomyces, Frankiales, Bradyrhizobium, and Luteimonas, while strain WRS8 significantly decreased the relative abundances of the dominant populations of Gemmatimonadaceae, Nitrospira, Haliangium, Paenibacillus, Massilia, Bryobacter, and Rokubacteriales and the rare bacterial populations of Enterorhabdus, Roseburia, Luteibacter, and Planifilum in the rhizosphere soils, compared with the controls. Significantly negative correlations were observed between the available metal concentrations and the abundances of Pseudomonas, Luteimonas, Frankiales, and Planifilum. These results implied that strain WRS8 could affect the abundances of the dominant and rare bacterial populations involved in metal immobilization, resulting in increased pH values and decreased metal availability and uptake in the vegetables in the contaminated soil.

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Data availability

The datasets used in the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the National Natural Science Foundation of China (41977199) and the Social Development Program of Jiangsu Province (BE2016744).

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Authors and Affiliations

  1. College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, Nanjing, 210095, China

    Yanyan Ge, Zhenyu Wen, Linyan He & Xiafang Sheng

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  1. Yanyan Ge
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  2. Zhenyu Wen
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  3. Linyan He
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  4. Xiafang Sheng
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Contributions

Yanyan Ge: investigation, formal analysis, methodology, writing—original draft. Zhenyu Wen: investigation, methodology, formal analysis. Linyan He: formal analysis. Xiafang Sheng: conceptualization, formal analysis, writing—review & editing, supervision.

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Correspondence to Xiafang Sheng.

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Highlights

• Strain WRS8 reduced Cd and Pb uptake in coriander cultivars in metal-polluted soil.

• Coriander Cd and Pb contents treated with strain WRS8 met the metal limit standard.

• WRS8 increased soil dominant bacterial group abundances and rare group compositions.

• Group abundances induced by WRS8 were negative correlations to metal availability.

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Supplementary file1 (DOCX 4036 KB)

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Ge, Y., Wen, Z., He, L. et al. Metal-immobilizing Pseudomonas taiwanensis WRS8 reduces heavy metal accumulation in Coriandrum sativum by changing the metal immobilization–related bacterial population abundances. Environ Sci Pollut Res 30, 76911–76922 (2023). https://doi.org/10.1007/s11356-023-27967-2

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