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The carbon and nitrogen metabolisms of Ardisia quinquegona were altered in different degrees by canopy and understory nitrogen addition in a subtropical forest

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Abstract

Although effects of atmospheric nitrogen (N) deposition on forest plants have been widely investigated, N interception and absorption effects by forest canopy should not be neglected. Moreover, how N deposition change the molecular biological process of understory dominant plants, which was easily influenced by canopy interception so as to further change physiological performance, remains poorly understood. To assess the effects of N deposition on forest plants, we investigated the effects of understory (UAN) and canopy N addition (CAN) on the transcriptome and physiological properties of Ardisia quinquegona, a dominant subtropical understory plant species in an evergreen broad-leaved forest in China. We identified a total of 7394 differentially expressed genes (DEGs). Three of these genes were found to be co-upregulated in CAN as compared to control (CK) after 3 and 6 h of N addition treatment, while 133 and 3 genes were respectively found to be co-upregulated and co-downregulated in UAN as compared to CK. In addition, highly expressed genes including GP1 (a gene involved in cell wall biosynthesis) and STP9 (sugar transport protein 9) were detected in CAN, which led to elevated photosynthetic capacity and accumulation of protein and amino acid as well as decrease in glucose, sucrose, and starch contents. On the other hand, genes associated with transport, carbon and N metabolism, redox response, protein phosphorylation, cell integrity, and epigenetic regulation mechanism were affected by UAN, resulting in enhanced photosynthetic capacity and carbohydrates and accumulation of protein and amino acid. In conclusion, our results showed that the CAN compared to UAN treatment had less effects on gene regulation and carbon and N metabolism. Canopy interception of N should be considered through CAN treatment to simulate N deposition in nature.

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Data availability

All sequence data were uploaded into the BioProject database hosted by the National Genomics Data Center (NGDC) under the BioProject RJCA009416.

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Funding

This study is supported by the National Natural Science Foundation of China (31971622) and the Youth Innovation Promotion Association of the Chinese Academy of Sciences (Y202077).

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Authors and Affiliations

  1. Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Liyuan Wang, Shuhua Wu, Xuncheng Liu & Nan Liu

  2. College of Life Sciences, Gannan Normal University, Ganzhou, 341000, China

    Shuhua Wu & Nan Liu

  3. South China National Botanical Garden, Guangzhou, 510650, China

    Xuncheng Liu & Nan Liu

  4. University of Chinese Academy of Sciences, Beijing, 100049, China

    Shuhua Wu

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  1. Liyuan Wang
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  2. Shuhua Wu
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  4. Nan Liu
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Contributions

Liyuan Wang, Shuhua Wu, Xuncheng Liu, and Nan Liu conceived and designed the experiments; Liyuan Wang and Shuhua Wu carried out the experiments; Liyuan Wang, Xuncheng Liu, and Nan Liu wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Xuncheng Liu or Nan Liu.

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Wang, L., Wu, S., Liu, X. et al. The carbon and nitrogen metabolisms of Ardisia quinquegona were altered in different degrees by canopy and understory nitrogen addition in a subtropical forest. Environ Sci Pollut Res 30, 57653–57666 (2023). https://doi.org/10.1007/s11356-023-26478-4

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  • DOI: https://doi.org/10.1007/s11356-023-26478-4

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