Abstract
In this study, a new microalgal strain, Asterarcys quadricellulare R-56, was isolated for biomass and lipid production. The effects of carbon and nitrogen sources and initial pH on the cell growth and lipid accumulation of strain R-56 were investigated. At 10 g L−1 glucose, 0.6 g L−1 sodium nitrate, and pH 7, the highest biomass of 4.18 g L−1 and lipid content of 43.66% were obtained. Microalgae had a broad pH tolerance in the range of 5–11, and the pH of the culture medium was close to neutral at the end of cultivation. The maximum contents of chlorophyll, carbohydrate, and protein under the recommended culture conditions were 19.47 mg mL−1, 21.80%, and 29.94%, respectively. Palmitic and palmitoleic acid contents in strain R-56 accounted for as high as 83.73% of total fatty acids. This study suggested that strain R-56 was a promising lipid producer for high-quality biodiesel production.
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Acknowledgements
The authors thank the State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology) for providing the research facilities.
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This work was supported by the Key Research and Development Program of Heilongjiang Province (No. 2022ZX02C15), the Natural Science Foundation of Heilongjiang Province (No. YQ2022E008), the Academic Backbone Project of Northeast Agricultural University (No. 54917412), and the Heilongjiang Touyan Innovation Team Program.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Xueting Song. Strain selection was performed by Hong-Yu Ren. The first draft of the manuscript was written by Hong-Yu Ren and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Ren, HY., Song, X., Kong, F. et al. Lipid production characteristics of a newly isolated microalga Asterarcys quadricellulare R-56 as biodiesel feedstock. Environ Sci Pollut Res 30, 48339–48350 (2023). https://doi.org/10.1007/s11356-023-25728-9
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DOI: https://doi.org/10.1007/s11356-023-25728-9