Abstract
Deca brominated diphenyl ether (BDE-209) is a widely used flame retardant with endocrine-disrupting activity which reportedly caused sperm quality decline and damaged blood-testis barrier (BTB). However, whether BDE-209 exposure led to BTB integrity dysfunction through affecting microtubule cytoskeletal organization and junctions was not well-elucidated. This study aimed to investigate the role of estrogen receptor α (ERα) in BDE-209-mediated perturbation of BTB integrity. Male rats and primary culture Sertoli cells were co-treated with BDE-209 and propylpyrazoletriol (PPT). The data demonstrated that BDE-209 impaired BTB integrity by reducing crucial tight junction-related proteins with ZO-1 and Occludin. Furthermore, the data suggested that BDE-209 diminished the apical ectoplasmic specialization markers with Eps8 and Formin1. In addition, BDE-209 damaged BTB ultrastructure including tight junctions and ectoplasmic specialization structures with broken tight junctions and the absence of actin microfilaments. Further experiments revealed that ERα was triggered in BDE-209-treated Sertoli cells. Unexpectedly, we found that PPT rescued BDE-209-mediated disruption of BTB integrity including tight junction and apical ectoplasmic specialization by activating ERα in Sertoli cells. Taken together, these findings indicated that intratesticular BDE-209 exposure perturbed BTB integrity and destroyed BTB structure by blocking ERα pathway. Our findings provide a new therapeutic target for male reproductive dysfunction.
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All data generated and analyzed during this study are included in this published article (and its supplementary information files).
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This work was supported by the National Natural Science Foundation of China (No. 82273598) and by the Nature Science Research Project of Anhui Province (No. 2108085MH305).
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Jinxia Zhai and Xiya Geng had the idea for the article. Xiya Geng, Yu Wei, and Wenfeng Geng conducted the experiment. Xiya Geng, Taifa Zhang, and Wenfeng Geng checked and managed the data. Xiya Geng, Yu Wei, and Tao Ding performed data analysis. Xiya Geng and Yu Wei drafted the manuscript. Jinxia Zhai, Huan He, Xin Gao, and Jixiang Xu provided expert review of the manuscript and reviewed and approved the manuscript. All authors contributed to the research article and approved the final version.
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Highlights
• BDE-209 exposure caused a decline in sperm quality.
• BDE-209 impaired blood-testis barrier integrity including tight junction and apical ectoplasmic specialization damage.
• BDE-209 induced elongated spermatid embedded in spermatogenic epithelium.
• BDE-209 induced blood-testis barrier dysfunction via estrogen receptor α.
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Geng, X., Wei, Y., Geng, W. et al. BDE-209 disrupted the blood-testis barrier integrity by inhibiting estrogen receptor α signaling pathway in Sprague–Dawley rats. Environ Sci Pollut Res 30, 47349–47365 (2023). https://doi.org/10.1007/s11356-023-25476-w
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DOI: https://doi.org/10.1007/s11356-023-25476-w