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Aspergillus sclerotiorum lipolytic activity and its application in bioremediation of high-fat dairy wastewater environments

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Abstract

Oils and grease (O&G) have low affinity for water and represent a class of pollutants present in the dairy industry. Enzyme-mediated bioremediation using biocatalysts, such as lipases, has shown promising potential in biotechnology, as they are versatile catalysts with high enantioselectivity and regioselectivity and easy availability, being considered a clean technology (white biotechnology). Specially in the treatment of effluents from dairy industries, these enzymes are of particular importance as they specifically hydrolyze O&G. In this context, the objective of this work is to prospect filamentous fungi with the ability to synthesize lipases for application in a high-fat dairy wastewater environment. We identified and characterized the fungal species Aspergillus sclerotiorum as a good lipase producer. Specifically, we observed highest lipolytic activity (20.72 U g−1) after 96 h of fermentation using sunflower seed as substrate. The fungal solid fermented was used in the bioremediation in dairy effluent to reduce O&G. The experiment was done in kinetic from 24 to 168 h and reduced over 90% of the O&G present in the sample after 168 h. Collectively, our work demonstrated the efficiency and applicability of fungal fermented solids in bioremediation and how this process can contribute to a more sustainable wastewater pretreatment, reducing the generation of effluents produced by dairy industries.

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Authors and Affiliations

  1. Engineering and Exact Sciences Center, State University Western Paraná, Toledo, PR, Brazil

    Jaíne Daiane de Moura Dickel, Jéssyca Ketterine Carvalho, Paulo Menegotto dos Santos, Maria Luiza Fernandes Rodrigues, Marcia Regina Fagundes-Klen, Cleide Viviane Buzanello, Rosemeire Aparecida da Silva de Lucca & Mauricio Ferreira da Rosa

  2. Department of Engineering and Exact, Federal University of Paraná, Palotina, PR, Brazil

    Jéssyca Ketterine Carvalho

  3. Centre de Recherche du CHU de Québec – Université Laval, Axe Oncologie, Quebec, QC, G1V 4G2, Canada

    Maruhen Amir Datsch Silveira

  4. Centre de Recherche Sur Le Cancer de L’Université Laval, Quebec, QC, G1R 3S3, Canada

    Maruhen Amir Datsch Silveira

  5. Microbiology Department, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil

    Carlos Augusto Rosa, Susana Johann & Ana Raquel de Oliveira Santos

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  1. Jaíne Daiane de Moura Dickel
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Jéssyca Ketterine Carvalho, Jaíne Daiane de Moura Dickel, Maria Luiza Fernandes Rodrigues, Carlos Augusto Rosa, Susana Johann, and Ana Raquel de Oliveira Santos. The first draft of the manuscript was written by Jaíne Daiane de Moura Rodrigues, Jéssyca Ketterine Carvalho, Maria Luiza Fernandes Rodrigues, Maruhen Amir Datsch Silveira, Paulo Menegotto, Marcia Regina Fagundes-Klen, Cleide Viviane Buzanello-Martins, Rosemeire Aparecida da Silva De Lucca, and Mauricio Ferreira da Rosa, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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de Moura Dickel, J.D., Carvalho, J.K., Silveira, M.A.D. et al. Aspergillus sclerotiorum lipolytic activity and its application in bioremediation of high-fat dairy wastewater environments. Environ Sci Pollut Res 30, 35517–35527 (2023). https://doi.org/10.1007/s11356-022-24669-z

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