Abstract
Banana harvesting generates a large amount of banana pseudostem waste, which is generally burnt or thrown away, despite containing many nutrients. Bio-enriched organic fertilizer (BOF) was prepared from banana pseudostem sap (BPS), and it has been patented (Patent No. WO 2013/001478 Al). Several reports revealed that its application increases plant growth promotion of various horticulture crops. Apart from macro- and micronutrients, it also contained phytohormones. Hence, the present study aims to detect and quantify phytohormone in it. A novel method was developed to extract four phytohormones, viz., indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), gibberellic acid (GA3), and salicylic acid (SA) using single solvent from BPS and BOF. Extracted hormones were analyzed by ultrahigh-performance liquid chromatography coupled with heated electrospray ionization tandem mass spectrometry (UHPLC-HESI-MS/MS). BOF showed a higher concentration of IAA, IBA, GA3, and SA than BPS. Thus, this is the first time a method has been reported to extract and detect phytohormones from banana pseudostem sap.
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Harihar Vaidya, Chirag Desai, and Jayesh Patel designed the research. Harihar Vaidya, Vanrajsinh H. Solanki, and Rohan V. Kansara conducted experiments. Susheel Singh and Vanrajsinh H. Solanki analyzed all data. Vanrajsinh H. Solanki, Trupti K. Vyas, Rohan V. Kansara, and Hiren Patel wrote marked errors and confirmed the final draft of the manuscript.
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Vaidya, H., Solanki, V.H., Kansara, R.V. et al. Development of a novel method for multiple phytohormone analysis by UHPLC-MS/MS from bio-enriched organic fertilizer prepared using banana pseudostem sap waste. Environ Sci Pollut Res 30, 71482–71490 (2023). https://doi.org/10.1007/s11356-022-23941-6
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DOI: https://doi.org/10.1007/s11356-022-23941-6