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Transcriptional analysis of heavy metal P1B-ATPases (HMAs) elucidates competitive interaction in metal transport between cadmium and mineral elements in rice plants

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Abstract

Cadmium (Cd) pollution has become a major threat to crop production and quality globally. The heavy metal P1B-ATPases (HMAs) play a crucial role in metal transport in plants. In the present study, we investigated the interaction in metal transport by HMAs between Cd and mineral elements in rice plants. Rice seedlings were treated with cadmium nitrate either in the nutrient solution (“Cd+M”) or in the ultrapure water (“Cd-M”). Result showed that phytotoxicity of Cd to rice seedlings was evident from both Cd treatments, judged by relative growth rate (RGR), where more severe repression (p < 0.05) of RGR was observed in the “Cd-M” treatments than the “Cd+M” treatments. More Cd (p < 0.05) was accumulated in rice tissues from the “Cd-M” treatments than the “Cd+M” treatments, while there is a significant difference (p < 0.05) in distribution and translocation of mineral elements in rice tissues between the “Cd+M” and the “Cd-M” treatments. RT-qPCR analysis displayed that the expression patterns of HMAs related genes were quite different between “Cd+M” and “Cd-M” treatments, suggesting their different regulatory effects during the transport of Cd and mineral elements within rice plants. The competition in metal transport by HMAs mainly occurs between Cd and micro-elements of Zn and Cu in rice tissues during Cd exposure. Overall, this study provides new evidence to clarify the different translocation mechanisms of HMAs in metal transport between Cd and mineral elements in rice seedlings during Cd exposure.

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The datasets supporting the conclusions of this article can be obtained from the corresponding author on reasonable request.

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Funding

This work is financially supported by the Natural Science Foundation of Guangxi (No. 2018GXNSFDA281024).

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Authors and Affiliations

  1. College of Environmental Science & Engineering, Guilin University of Technology, Guilin, 541004, People’s Republic of China

    Peng Tian, Yu-Xi Feng, Cheng-Zhi Li, Ping Zhang & Xiao-Zhang Yu

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  1. Peng Tian
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Contributions

Conceptualization, Methodology, supervision, writing-reviewing and editing, and funding acquisition: Xiao-Zhang Yu. Investigation, data analysis, and visualization: Peng Tian. Writing original draft preparation and visualization: Yu-Xi Feng. Investigation: Cheng-Zhi Li. Investigation, Ping Zhang. All of the authors contributed to the final review of the manuscript.

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Correspondence to Xiao-Zhang Yu.

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Tian, P., Feng, YX., Li, CZ. et al. Transcriptional analysis of heavy metal P1B-ATPases (HMAs) elucidates competitive interaction in metal transport between cadmium and mineral elements in rice plants. Environ Sci Pollut Res 30, 287–297 (2023). https://doi.org/10.1007/s11356-022-22243-1

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