Abstract
Lentinus crinitus bioaccumulates lithium in mycelia, but bioaccumulation may be affected by pH of the culture medium. Lithium is used in clinical practice as a mood stabilizer and antidepressant. This study aimed to assess the effect of culture medium pH and lithium source (LiCl or Li2CO3) on lithium bioaccumulation in vegetative mycelia of L. crinitus grown in malt extract broth. Lentinus crinitus U9-1 was cultured in malt extract broth supplemented with Li2CO3 or LiCl (50 mg L−1 lithium) in the pH range of 3.0 to 6.0. The pH was adjusted using HCl solution. The results showed that medium pH affected mycelial biomass production, lithium bioaccumulation in mycelial biomass, and lithium transfer from the culture medium to mycelial biomass. The effect of lithium source on the bioaccumulation capacity of mycelial biomass varied according to pH. At pH 4.0, both lithium sources stimulated mycelial biomass production compared to the control without the addition of lithium. At pH 5.5, Li2CO3 provided the highest lithium bioaccumulation in mycelial biomass. Lithium transfer from the culture medium to mycelia was highest in Li2CO3-supplemented cultures at pH 4.5. LiCl reduced hyphal width compared with Li2CO3 and the control. However, pH and lithium sources did not affect the formation of clamp connections in hyphae. For the first time, the influence of the pH of the culture medium on lithium bioaccumulation by Lentinus crinitus is reported. Finally, we conclude that the culture medium pH affected lithium transfer and bioaccumulation in mycelial biomass differently depending on the lithium source. Additionally, we report the presence of clamp connections in the hyphae of L. crinitus as an indicator of even growth.
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The authors thank the Paranaense University, the Federal University of Bahia, Fundação Araucária, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES) -finance code 001-, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support and fellowship
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Conceived, designed, and wrote the paper: Faria, MGI; Linde, GA. Acquisition, analysis, and interpretation of data; drafting of the manuscript: Faria, MGI; Avelino, KV; Philadelpho, BO; Bomfim, RS; Valle, JS; Gonçalves-Junior, AC; Dragunski, DC; Ferreira, ES; Souza, CO; Ribeiro, CDF; Colauto, NB. Critical revision of the manuscript for important intellectual content: Colauto, NB. Coordinating the research project: Linde, GA.
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Faria, M.G.I., Avelino, K.V., Philadelpho, B.O. et al. Lithium bioaccumulation in Lentinus crinitus mycelia grown in media with different lithium sources and pH values. Environ Sci Pollut Res 29, 87519–87526 (2022). https://doi.org/10.1007/s11356-022-21893-5
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DOI: https://doi.org/10.1007/s11356-022-21893-5