Abstract
Plant growth-promoting bacteria and biochar have been widely used as immobilizers to remediate heavy metal contaminated soil. However, few studies have unraveled the effect and synergistic mechanism of combined application of plant growth-promoting bacteria and biochar on in situ heavy metal contaminated soil remediation and plant yield and quality improvement under heavy metal pollution stress. In this study, the effects of biochar, γ-PGA-producing bacteria (Bacillus amyloliquefaciens strain W25) and their combined application on Cd and Pb immobilization, γ-PGA production in soil filtrate, the bacterial community in rhizosphere soil, physicochemical properties of soil, heavy metal uptake, and quality and yield of tomato in heavy metal-contaminated soil were investigated. The application of W25, biochar, and their combinations significantly reduced Cd content in mature tomato fruits by 22–60%, increased the single fruit weight and lycopene content by 7–21% and 23–48%, respectively, and the combination of biochar and W25 had the best effect. All the treatments significantly reduced DTPA-Cd and DTPA-Pb contents in rhizosphere soil (42–53% and 6.5–35%), increased the pH value and the activities of urease-alkaline phosphatase of soil, but did not affect the expression of heavy metal transporter gene LeNRAMP1 in tomato roots. Biochar + W25 increased the relative abundance of plant growth-promoting bacteria such as Bacillus and Streptomyces. Biochar-enhanced plant growth-promoting bacteria to settle and colonize in soil significantly improved the ability of strain W25 to produce γ-PGA, and immobilized Cd in soil filtrate. The combination of biochar and plant growth-promoting bacteria ensures safe crop production in heavy metal-contaminated soil.
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Funding
This study was financially supported by the Integration of Science and Education Program Foundation for the Talents by Qilu University of Technology, Shandong Academy of Sciences (No.2018–81110268); Foundation of Key Laboratory of Pulp and Paper Science and Technology of Ministry of Education/Shandong Province of China (No. KF201817); and Foundation of State Key Laboratory of Biobased Material and Green Papermaking (No. 2419010205, No. 23190444 and No. ZZ20200130).
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Xingwang Liu: conceptualization, methodology, investigation, data curation, writing—original draft. Xiaohan Wang: methodology, investigation. Tianyu Xu: methodology, investigation. Haizheng Ma methodology, investigation. Tao Xia: supervision, conceptualization, writing—review and editing, funding acquisition.
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Liu, X., Wang, X., Xu, T. et al. The combined application of γ-PGA-producing bacteria and biochar reduced the content of heavy metals and improved the quality of tomato (Solanum lycopersicum L.). Environ Sci Pollut Res 29, 88938–88950 (2022). https://doi.org/10.1007/s11356-022-21842-2
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DOI: https://doi.org/10.1007/s11356-022-21842-2