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Bio-efficacy of Geobacillus thermodenitrificans PS41 against larvicidal, fungicidal, and plant growth–promoting activities

  • Recent Trends on Sustainable Agriculture through Environmental Friendly Pesticides: An Ecological Conservation Approach
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Abstract

The microbial interactions with plant hosts were known to establish plant growth and beneficial productivity. Some bacteria, fungi, actinomycetes, yeast, and algae have proven as potential effective microbes in agricultural field. In this study, the insecticidal effect of Geobacillus thermodenitrificans PS41 secondary metabolites was tested against third instar larvae of Spodoptera litura, with mortality rate 60.26 ± 1.5% which might influence the agropest management. The test bacterial metabolites were subjected to GC-MS analysis. Totally, 17 different compounds were identified from the ethyl acetate extract metabolites of PS41 strain. The highest peak was obtained with behenic alcohol compound followed by 1-octadecene and penta erythrityl tetrachloride. The Geobacillus thermodenitrificans PS41 secondary metabolites showed potential antifungal activity against plant pathogenic fungi. The highest inhibit was attained against Cladosporium sp., (25 mm) followed by Rhizoctonia solani and Alternaria brassicola (23 mm). However, no toxic effect was exerted upon earthworm (Perionyx excavatus) when treated with PS41 bacterial metabolites. The potential PS41 strain was also found supporting the plant growth. The potential bacterial strain PS41 did not show antagonistic activity against soil bacteria such as Rhizobium sp., Azotobacter sp., Azospirullum brasilense, Pseudomonas fluorescens and Bacillus megaterium. The potential test organism, Geobacillus thermodenitrificans PS41, possessing biopesticide and biofertilizer properties can be a suitable ecofriendly organic applicant in agricultural field for enhancing crop production.

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Supporting data presented in this study are available on request from the corresponding author

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Acknowledgements

DST-FIST, New Delhi, India, granted sophisticated instrumentation facility with reference number SR/ FST/ LSI-640/ 2015 (C) dated 30/5/2016 and financial support under DBT (BT/PR 5426/AAQ/3/599/2012) New Delhi, Virbac Animal Health India Pvt. Ltd. (VIRBAC - Consultancy project sanction order, Date: 30.10.2018), Mumbai, in the form of fellowship.

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  1. Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, 11, India

    Nagarajan Siddharthan, Ramasamy Balagurunathan & Natarajan Hemalatha

  2. Department of Environmental Science, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, 11, India

    Srinivasan Venkatesan

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  1. Nagarajan Siddharthan
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  2. Ramasamy Balagurunathan
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Dr. Hemalatha N (Corresponding author) — research idea, work plan and paper work

Dr. Siddharthan N (First author) — implementation and result data collection, manuscript typing

Dr. Balagurunathan R — chemical and instrumental facilities, paper editing

Dr. Venkatesan S — data analysis

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Correspondence to Natarajan Hemalatha.

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Siddharthan, N., Balagurunathan, R., Venkatesan, S. et al. Bio-efficacy of Geobacillus thermodenitrificans PS41 against larvicidal, fungicidal, and plant growth–promoting activities. Environ Sci Pollut Res 30, 42596–42607 (2023). https://doi.org/10.1007/s11356-022-20455-z

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