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DSCAM-AS1 promotes cervical carcinoma cell proliferation and invasion via sponging miR-338-3p

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Abstract

Deregulated lncRNA DSCAM-AS1 expression was found in several tumors. However, mechanism and functional role of DSCAM-AS1 in cervical carcinoma remain unknown. DSCAM-AS1 was detected in cervical carcinoma specimens and cells by RT-qPCR. CCK-8, Matrigel transwell, and flow cytometry were conducted to determine cell functions. In this research, we firstly we explored DSCAM-AS1 expression in cervical carcinoma cells and specimens. We revealed that DSCAM-AS1 was upregulated in cervical carcinoma lines (C4-1, Caski, Hela, and Siha) compared to GH329 cells. DSCAM-AS1 was upregulated in cervical carcinoma specimens compared to control no-tumor specimens. Overexpression of DSCAM-AS1 induced cervical carcinoma cell growth and cycle. Moreover, our data revealed that miR-338-3p expression was downregulated in cervical carcinoma cells and specimens. There was a negative correlation between miR-338-3p expression and DSCAM-AS1 expression in cervical carcinoma specimens. Elevated expression of miR-338-3p decreased cervical carcinoma cell growth and cycle and invasion. Furthermore, luciferase reporter analysis revealed that miR-338-3p overexpression suppressed luciferase activity of WT-DSCAM-AS1 vector but not the mut-DSCAM-AS1. Ectopic expression of DSCAM-AS1 decreased miR-338-3p expression in the Siha cell. Overexpression of DSCAM-AS1 promoted cervical carcinoma cell growth and cycle via regulating miR-338-3p. These results suggested that DSCAM-AS1 functions as one oncogene through sponging miR-338-3p in cervical carcinoma.

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Authors and Affiliations

  1. International Medical Center, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China

    Chunmei Li, Xiaoqiong Li, Liangzhi Wu & Jingjun He

  2. Department of Traditional Chinese Medicine, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China

    Chunmei Li, Xiaoqiong Li, Yi Zhang, Liangzhi Wu, Jingjun He, Nan Jiang, Hui Zhao & Wenwen Liu

  3. Department of Gynecology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China

    Yi Zhang, Nan Jiang, Hui Zhao & Wenwen Liu

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  1. Chunmei Li
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Correspondence to Chunmei Li.

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Informed consent was gotten from each patient, and our protocol agreed with Research Ethics Committee of Guangdong Second Provincial General Hospital.

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Li, C., Li, X., Zhang, Y. et al. DSCAM-AS1 promotes cervical carcinoma cell proliferation and invasion via sponging miR-338-3p. Environ Sci Pollut Res 29, 58906–58914 (2022). https://doi.org/10.1007/s11356-022-19962-w

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