Abstract
Microplastics are one of the most widely discussed environmental issues worldwide. Several studies have shown the effect of microplastic exposure on the marine environment; however, studies on freshwater systems are lacking. This study was conducted to investigate the effect of microplastics on hydroponically growing emergent freshwater macrophytes, watermilfoil (sp. roraima) under controlled environmental conditions. Plants were exposed to 0 mg L−1 (control), 0.05 mg L−1, 0.25 mg L−1, 1.25 mg L−1, and 6 mg L−1 of 3-µm polystyrene microspheres for 7 days. The oxidative stress, antioxidant response, pigmentations, Fv/Fm, and growth parameters in the above-water and below-water parts were analyzed separately. Microscopic observations were performed to confirm the tissue absorbance of the microplastics. Exposure to microplastics altered some parameters; however, growth was not affected. The effect of microplastics was not linear with the exposure concentration for most of the parameters and between 1.25 and 6 mg L−1 concentrations. The response trends mostly followed the second-order polynomial distributions. Under the 1.25 mg L−1 exposure, there were significant changes in root length, H2O2 content, catalase activity, anthocyanin content, and Fv/Fm. There were differences in parameters between the above-water and below-water parts, and the responses of the microplastics followed different trends. Microscopic observations confirmed the attachment of microplastic particles onto newly formed roots, except for older roots or shoot tissues.
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References
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126. https://doi.org/10.1016/S0076-6879(84)05016-3
Bellasi A, Binda G, Pozzi A, et al (2020) Microplastic contamination in freshwater environments: a review, focusing on interactions with sediments and benthic organisms. Environ - MDPI 7:. https://doi.org/10.3390/environments7040030
Cechin I, Corniani N, de Fumis T, F, Cataneo AC, (2010) Differential responses between mature and young leaves of sunflower plants to oxidative stress caused by water deficit. Cienc Rural 40:1290–1294
Davies TW, Jenkins SR, Kingham R et al (2012) Extirpation-resistant species do not always compensate for the decline in ecosystem processes associated with biodiversity loss. J Ecol 100:1475–1481. https://doi.org/10.1111/j.1365-2745.2012.02012.x
De Souza Machado AA, Lau CW, Kloas W et al (2019) Microplastics can change soil properties and affect plant performance. Environ Sci Technol 53:6044–6052. https://doi.org/10.1021/acs.est.9b01339
Dovidat LC, Brinkmann BW, Vijver MG, Bosker T (2020) Plastic particles adsorb to the roots of freshwater vascular plant Spirodela polyrhiza but do not impair growth. Limnol Oceanogr Lett 5:37–45. https://doi.org/10.1002/lol2.10118
Downing AS, van Nes EH, Mooij WM, Scheffer M (2012) The resilience and resistance of an ecosystem to a collapse of diversity. PLoS One 7:. https://doi.org/10.1371/journal.pone.0046135
Hartmann NB, Hüffer T, Thompson RC et al (2019) Are we speaking the same language? Recommendations for a definition and categorization framework for plastic debris. Environ Sci Technol 53:1039–1047. https://doi.org/10.1021/acs.est.8b05297
Horton AA, Barnes DKA (2020) Microplastic pollution in a rapidly changing world: implications for remote and vulnerable marine ecosystems. Sci Total Environ 738:140349. https://doi.org/10.1016/j.scitotenv.2020.140349
Horton AA, Walton A, Spurgeon DJ et al (2017) Microplastics in freshwater and terrestrial environments: evaluating the current understanding to identify the knowledge gaps and future research priorities. Sci Total Environ 586:127–141. https://doi.org/10.1016/j.scitotenv.2017.01.190
Jägerbrand AK, Kudo G (2016) Short-term responses in maximum quantum yield of PSII (Fv/Fm) to ex situ temperature treatment of populations of bryophytes originating from different sites in Hokkaido, Northern Japan. Plants 5:455–465. https://doi.org/10.3390/plants5020022
Kataoka T, Nihei Y, Kudou K, Hinata H (2019) Assessment of the sources and inflow processes of microplastics in the river environments of Japan. Environ Pollut 244:958–965. https://doi.org/10.1016/j.envpol.2018.10.111
Lambert S, Wagner M (2018) Microplastics are contaminants of emerging concern in freshwater environments: an overview. In: Handbook of Environmental Chemistry. pp 1–23
Lebreton L, Andrady A (2019) Future scenarios of global plastic waste generation and disposal. Palgrave Commun 5:1–11. https://doi.org/10.1057/s41599-018-0212-7
Lee KW, Shim WJ, Kwon OY, Kang JH (2013) Size-dependent effects of micro polystyrene particles in the marine copepod Tigriopus japonicus. Environ Sci Technol 47:11278–11283. https://doi.org/10.1021/es401932b
Li Z, Li Q, Li R et al (2020) Physiological responses of lettuce (Lactuca sativa L.) to microplastic pollution. Environ Sci Pollut Res 27:30306–30314. https://doi.org/10.1007/s11356-020-09349-0
Liu Y, Li M, Xu J, et al (2019) Physiological and metabolomics analyses of young and old leaves from wild and cultivated soybean seedlings under low-nitrogen conditions. BMC Plant Biol. 19
Lozano YM, Rillig MC (2020) Effects of microplastic fibers and drought on plant communities. Environ Sci Technol 54:6166–6173. https://doi.org/10.1021/acs.est.0c01051
MacAdam JW, Nelson CJ, Sharp RE (1992) Peroxidase activity in the leaf elongation zone of tall fescue : I. spatial distribution of ionically bound peroxidase activity in genotypes differing in length of the elongation zone. Plant Physiol 99:872–878
Mateos-Cárdenas A, Scott DT, Seitmaganbetova G et al (2019) Polyethylene microplastics adhere to Lemna minor (L.), yet have no effects on plant growth or feeding by Gammarus duebeni (Lillj.). Sci Total Environ 689:413–421. https://doi.org/10.1016/j.scitotenv.2019.06.359
Meng F, Yang X, Riksen M, et al (2021) Response of common bean (Phaseolus vulgaris L.) growth to soil contaminated with microplastics. Sci. Total Environ. 755
Murchie EH, Lawson T (2013) Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications. J Exp Bot 64:3983–3998. https://doi.org/10.1093/jxb/ert208
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880. https://doi.org/10.1093/oxfordjournals.pcp.a076232
Nakata M, Ohme-Takagi M (2014) Quantification of anthocyanin content. Bio-Protocol 4:. https://doi.org/10.21769/bioprotoc.1098
Nayek S, Gupta S, Saha R (2010) Effects of metal stress on biochemical response of some aquatic macrophytes growing along an industrial waste discharge channel. J Plant Interact 5:91–99. https://doi.org/10.1080/17429140903282904
Rillig MC, Lehmann A, de Souza Machado AA, Yang G (2019) Microplastic effects on plants. New Phytol 223:1066–1070. https://doi.org/10.1111/nph.15794
Sachdev S, Ansari SA, Ansari MI, Fujita M (2021) Abiotic stress and reactive oxygen species : generation, signaling, and defense mechanisms. Antioxidants 10:277
Satterfield CN, Bonnell AH (1955) Interferences in the titanium sulfate method for hydrogen peroxide. Anal Chem 27:1174–1175. https://doi.org/10.1021/ac60103a042
Scudo A, Liebmann B (EEA), Corden C, et al (2017) Intentionally added microplastics in products. Amec Foster Wheel 220
Senavirathna MDHJ, Muhetaer G, Atapaththu KSS, Fujino T (2021) Egeria densa allelopathy on microcystis aeruginosa under different light intensities and preliminary insight into inter-parameter relationships. Water Air Soil Pollut 232:1–13. https://doi.org/10.1007/s11270-021-05088-1
Senavirathna MDHJ, Muhetaer G, Zhaozhi L, Fujino T (2020a) Allelopathic influence of low concentration Microcystis aeruginosa on Egeria densa under different light intensities. Chem Ecol 36:903–921. https://doi.org/10.1080/02757540.2020.1798939
Senavirathna MDHJ, Wijesinghe NA, Liu Z, Fujino T (2020b) Effects of short-term exposure to different salinity levels on Myriophyllum spicatum and Ceratophyllum demersum and suitability of biomarkers to evaluate macrophyte responses to salinity stress Ann Limnol 56: https://doi.org/10.1051/limn/2020021
Shafiq M, Qadir A, Hussain CM (2019) Microplastics as contaminant in freshwater ecosystem: a modern environmental issue. In: Hussain CM (ed) Handbook of Environmental Materials Management. Springer International Publishing, Cham, pp 355–377
Teng S, Keurentjes J, Bentsink L et al (2005) Sucrose-specific induction of anthocyanin biosynthesis in Arabidopsis requires the MYB75/PAP1 gene. Plant Physiol 139:1840–1852. https://doi.org/10.1104/pp.105.066688
Thompson RC, Moore CJ, Saal FSV, Swan SH (2009) Plastics, the environment and human health: current consensus and future trends. Philos Trans R Soc B Biol Sci 364:2153–2166. https://doi.org/10.1098/rstb.2009.0053
van Weert S, Redondo-Hasselerharm PE, Diepens NJ, Koelmans AA (2019) Effects of nanoplastics and microplastics on the growth of sediment-rooted macrophytes. Sci Total Environ 654:1040–1047. https://doi.org/10.1016/j.scitotenv.2018.11.183
Wagner M, Scherer C, Alvarez-Muñoz D et al (2014) Microplastics in freshwater ecosystems: what we know and what we need to know. Environ Sci Eur 26:1–9. https://doi.org/10.1186/s12302-014-0012-7
Wellburn AR (1994) The spectral determination of Chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144:307–313. https://doi.org/10.1016/S0176-1617(11)81192-2
Yu H, Zhang X, Hu J et al (2020) Ecotoxicity of polystyrene microplastics to submerged carnivorous Utricularia vulgaris plants in freshwater ecosystems. Environ Pollut 265:114830. https://doi.org/10.1016/j.envpol.2020.114830
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The authors would like to thank the Japanese Society for the Promotion of Science (JSPS) for grant allocation.
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This research was funded by the Japanese Society for the Promotion of Science (JSPS), JSPS KAKENHI grant number 21K14248.
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M. D. H. J. S. conceived and designed the experiments; M. D. H. J. and L. Z. performed experiments and analyzed data; M. D. H. J. S. acquired the funding; M. D. H. J. S. and T. F. contributed to the resources; M. D. H. J. S. wrote the first draft manuscript; M. D. H. J. S., L. Z., and T. F. reviewed the manuscript.
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Senavirathna, M.D.H.J., Zhaozhi, L. & Fujino, T. Short-duration exposure of 3-µm polystyrene microplastics affected morphology and physiology of watermilfoil (sp. roraima). Environ Sci Pollut Res 29, 34475–34485 (2022). https://doi.org/10.1007/s11356-022-18642-z
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DOI: https://doi.org/10.1007/s11356-022-18642-z