Abstract
The microorganisms and allelochemicals in Pontederia cordata planting water may have a synergistic inhibitory effect on algae. To study this synergy, an algae-inhibiting organism was isolated and identified, and its growth and feeding characteristics were studied. The organism was identified as Poterioochromonas malhamensis yzs924 based on both its morphology and molecular barcoding employing 18S rDNA gene sequences.
The growth and feeding of P. malhamensis were affected by environmental factors and the state of its prey. (1) P. malhamensis is a mixotrophic flagellate. Its heterotrophic growth was the fastest in a wheat grain medium, and its growth rate in this study reached 2.5 day−1. (2) Within a short period of time (2 days), P. malhamensis growth was slower under continuous dark conditions than under alternating light and dark conditions, but it fed on Microcystis aeruginosa more rapidly under dark conditions. (3) High pH was disadvantageous to the growth and grazing of P. malhamensis. When the pH was kept stable at 9, P. malhamensis could not grow continuously. (4) When the initial density of M. aeruginosa was 5 × 107 cells/mL or is in a period of decline, P. malhamensis could not remove all M. aeruginosa. The combined use of P. malhamensis and allelochemicals may represent a method of M. aeruginosa control, but this approach requires further research.
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Thanks to Dr. Ling Lin for his help in species identification.
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The research was funded by the National Natural Science Foundation of China through Grant 31170443.
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All authors contributed to the study conception and design. Tingting Zhang provided support for experimental design, manuscript revision, and funding. Material preparation, data collection, and analysis were performed by Hao Yan, Qin Li, Bo Chen, and Mei Shi. The first draft of the manuscript was written by Hao Yan, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Yan, H., Li, Q., Chen, B. et al. Identification and feeding characteristics of the mixotrophic flagellate Poterioochromonas malhamensis, a microalgal predator isolated from planting water of Pontederia cordata. Environ Sci Pollut Res 29, 40599–40611 (2022). https://doi.org/10.1007/s11356-022-18614-3
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DOI: https://doi.org/10.1007/s11356-022-18614-3