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Conservation state of two paintings in the Santa Margherita cliff cave: role of the environment and of the microbial community

  • The Interaction Between Environmental Pollution and Cultural Heritage: From Outdoor to Indoor
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract   

The conservation of ancient paintings sited in humid environments is an actual challenge for restorers, because it needs the knowledge of the materials the paintings are made up and of their interaction with a peculiar surrounding environment; thus, tailored procedures and strategies aimed at restoring and preserving paintings are necessary. Santa Margherita’s cave in Castellammare del Golfo (Trapani, Italy) is a natural cave, containing the remains of paintings, in a poor state of conservation, belonging to an ancient church dated back to the Middle Age. The present manuscript reports the monitoring of environmental conditions (i.e., temperature and humidity) in a full year, as well as a study on the materials constituting the stone support and the paintings together with a survey of the microbial community. The findings allow us to define the causes that mainly involve the degradation of the paintings. In detail, the degradation of the east and the west walls occurred differently because of the exposure to the sea aerosol, which influenced the salt composition, also contributing to diversifying the bacterial community. Some specific actions to plan the conservation and restoration of paintings and to preserve the site are suggested.

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Data availability

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

References

  • Abdel-Aleem H, Dishisha T, Saafan A et al (2019) Biocementation of soil by calcite/aragonite precipitation using Pseudomonas azotoformans and Citrobacterfreundii derived enzymeshttps://doi.org/10.1039/C9RA02247C

  • Achal V, Mukherjee A, Reddy M (2011) Effect of calcifying bacteria on permeation properties of concrete structures. J Ind Microbiol Biot 38:1229–1234. https://doi.org/10.1007/s10295-010-0901-8

    Article  CAS  Google Scholar 

  • Albertano P (1993) Epilithic algal communities in hypogean environments. G Bot Ital 127(3):386–438

    Article  Google Scholar 

  • Alonso L, Pommier T, Kaufmann B et al (2019) Anthropization level of Lascaux Cave microbiome shown by regional-scale comparisons of pristine and anthropized caves. Mol Ecol 28(14):3383–3394. https://doi.org/10.1111/mec.15144

    Article  Google Scholar 

  • Anne S, Rozenbaum O, Andreazza P, Rouet J (2010) Evidence of a bacterial carbonate coating on plaster samples subjected to the calcite bioconcept biomineralization technique. Constr Build Mater 24:1036–1042. https://doi.org/10.1016/j.conbuildmat.2009.11.014

    Article  Google Scholar 

  • Aouad G, Crovisier JL, Damidot D et al (2008) Interactions between municipal solid waste incinerator bottom ash and bacteria (Pseudomonas aeruginosa). Sci Total Environ 393:385–393

    Article  CAS  Google Scholar 

  • Armetta F, Nardo VM, Trusso S et al (2021) The silver collection of San Gennaro treasure (Neaples): a multivariate statistic approach applied to X-ray fluorescence data. Spectrochim Acta Part B Atomic Spectroscopy 180:106171

    Article  CAS  Google Scholar 

  • Aubert M, Setiawan P, Oktaviana AA, Brumm A et al (2018) V Palaeolithic cave art in Borneo. Nature 564:254–257

    Article  CAS  Google Scholar 

  • Belfiore CM, Barca D, Bonazza A et al (2013) Application of spectrometric analysis to the identification of pollution sources causing cultural heritage damage. Environ Sci Pollut Res 20:8848–8859. https://doi.org/10.1007/s11356-013-1810-y

    Article  CAS  Google Scholar 

  • Callahan BJ, McMurdie PJ, Rosen MJ et al (2016) DADA2: high-resolution sample inference from Illumina amplicon data. Nat Methods 13:581–583. https://doi.org/10.1038/nmeth.3869

    Article  CAS  Google Scholar 

  • Caneva G, Tescari M, Bartoli F, Nugari MP, Pietrini AM, Salvadori O (2017) Ecological Mapping for the Preventive Conservation of Prehistoric Mural Paintings in Rock Habitats: the Site of Filiano (Basilicata, Italy). Conserv Sci Cult Herit 15(2):53–59. https://doi.org/10.6092/issn.1973-9494/7118

    Article  Google Scholar 

  • Caneva G, Tescari M, Bartoli F, Nugari MP, Pietrini AM, Salvadori O (2015). Ecological Mapping for the Preventive Conservation of Prehistoric Mural Paintings in Rock Habitats: the Site of Filiano (Basilicata, Italy). Conservation Science in Cultural Heritage 15(2):53–59. https://doi.org/10.6092/issn.1973-9494/7118

  • Caneva G, Bartoli F, Fontani M, Mazzeschi D, Visca P (2019) Changes in biodeterioration patterns of mural paintings: multi-temporal mapping for a preventive conservation strategy in the Crypt of the Original Sin (Matera, Italy). J Cult Herit 40:59–68

    Article  Google Scholar 

  • Caponetti E, Armetta F, Ciaramitaro V et al (2021) Effectiveness of some protective and self-cleaning treatment: a challenge for the conservation of the Temple G in Selinunte. Prog Surf Coat 151:106020

    CAS  Google Scholar 

  • Carretti E, Giorgi R, Berti D, Baglioni P (2007) Oil-in-water nanocontainers as low environmental impact cleaning tools for works of art: Two case studies. Langmuir 23(11):6396–6403

    Article  CAS  Google Scholar 

  • Charola AE (2000) Salt in the deterioration of porous materials. J Am Inst Conserv 39:327–343

    Article  Google Scholar 

  • Chen Q, Liu Z, Peng Q et al. (2010) Diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from Xiaoxi National Natural Reserve, Hunan Province. 50(11):1452-9

  • Comite V, Álvarez de Buergo M, Barca D et al (2017) Damage monitoring on carbonate stones: field exposure tests contributing to pollution impact evaluation in two Italian sites. Constr Build Mater 152:907–922. https://doi.org/10.1016/j.conbuildmat.2017.07.048

    Article  CAS  Google Scholar 

  • Corral P, Amoozegar MA, Ventosa A (2020) Halophiles and their biomolecules: recent advances and future applications in biomedicine. Mar Drugs 18:33. https://doi.org/10.3390/md18010033

    Article  CAS  Google Scholar 

  • Corvo F, Reyes J, Valdes C et al (2010) Influence of air pollution and humidity on limestone materials degradation in historical buildings located in cities under tropical coastal climates. Water Air Soil Pollut 205:359. https://doi.org/10.1007/s11270-009-0081-1

    Article  CAS  Google Scholar 

  • Costa EAL, Campos VP, da Silva Filho LCP, Greven HA (2009) Evaluation of the aggressive potential of marine chloride and sulfate salts on mortars applied as renders in the Metropolitan Region of Salvador – Bahia. Brazil J Environ Manage 90:1060–1068

    Article  CAS  Google Scholar 

  • Coy MR, Hoffmann M, Kingdom Gibbard HN et al (2014) Nested-quantitative PCR approach with improved sensitivity for the detection of low titer levels of Candidatus Liberibacter asiaticus in the Asian citrus psyllid Diaphorina Citri Kuwayama. J Microbiol Methods 102:15–22. https://doi.org/10.1016/j.mimet.2014.04.007

    Article  CAS  Google Scholar 

  • Creer KM, Kopper JS (1974) Paleomagnetic dating of cave paintings in Tito Bustillo Cave, Asturias, Spain. Science 186:348–350

    Article  CAS  Google Scholar 

  • De Muynck W, Verbeken K, De Belie N, Verstraete W (2013) Influence of Temperature on the Effectiveness of a Biogenic Carbonate Surface Treatment for Limestone Conservation. Appl Microbiol Biot 97:1335–1347. https://doi.org/10.1007/s00253-012-3997-0

    Article  CAS  Google Scholar 

  • Dhami NK, Reddy MS, Mukherjee A (2014) Application of calcifying bacteria for remediation of stones and cultural heritages. Front Microbiol 5:304. https://doi.org/10.3389/fmicb.2014.003044071612

    Article  Google Scholar 

  • Dick J, De Windt W, De Graef B, Saveyn H, Van DM, De Belie N, Verstraete W (2006) Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species. Biodegradation 17:357–367. https://doi.org/10.1007/s10532-005-9006-x

    Article  CAS  Google Scholar 

  • Dresler C, Saladino ML, Demirbag C et al (2017) Development of controlled release systems of biocides for the conservation of cultural heritage. Int Biodeterior Biodegradation 125:150–156

    Article  CAS  Google Scholar 

  • Fairbridge R (1957) The dolomite question Regional Aspects of Carbonate Deposition Edited by Rufus. J Le Blanc and Julia g Breeding 5:125–178

    Google Scholar 

  • Galante M (2011) La grotta Santa Margherita Ministero dell’Istruzione, dell’Università e della Ricerca Accademia di Belle Arti di Palermo.

  • Gaona O, Cerqueda-Garcı´a D, Falco´n LI et al (2019) Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae. PLoS One 14(12):0226239. https://doi.org/10.1371/journal.pone.0226239

    Article  CAS  Google Scholar 

  • García-Vera VE, Tenza-Abril AJ, Solak AM, Lanzón M (2020) Calcium hydroxide nanoparticles coatings applied on cultural heritage materials: their influence on physical characteristics of earthen plasters. Appl Surf Sci 504:144195

    Article  CAS  Google Scholar 

  • Grassi S, Carretti E, Pecorelli P et al (2007) The conservation of the Vecchietta’s wall paintings in the Old Sacristy of Santa Maria della Scala in Siena: the use of nanotechnological cleaning agents. J Cult Herit 8(2):119–125

    Article  Google Scholar 

  • Hammer Ø, Harper DAT, Ryan PD (2001) PAST: Paleontological statistics software package for education and data analysis. Palaeontol Electron 4(1):9

    Google Scholar 

  • Harrison JJ, Ceri H, Stremick C, Turner RJ (2004) Biofilm susceptibility to metal toxicity. Environ Microbiol 6:1220–1227

    Article  CAS  Google Scholar 

  • Helen H (2003) Pigments of English medieval wall painting archetype publications. London

  • Hersman LE, Huang A, Maurice PA, Jennifer H (2011) Forsythe siderophore production and iron reduction by Pseudomonas mendocina in response to iron deprivation. Geomicrobiol J 4:261–273. https://doi.org/10.1080/01490450050192965

    Article  Google Scholar 

  • Kim HK, Park SJ, Han JI, Lee HK (2013) Microbially mediated calcium carbonate precipitation on normal and lightweight concrete. Constr Build Mater 38:1073–1082. https://doi.org/10.1016/j.conbuildmat.2012.07.040

    Article  CAS  Google Scholar 

  • La Russa MF, Rovella N, Pelosi C et al (2016) A multi-analytical approach applied to the archaeometric study of mortars from the Forty Martyrs rupestrian complex in Cappadocia (Turkey). Microchem J 125:34–42

    Article  CAS  Google Scholar 

  • Laiz L, Recio D, Hermosin Saiz-Jimenez BC (2000) Microbial communities in salt efflorescences. In Of Microbes and Art, the Role of Microbial Communities in the Degradation and Protection of Cultural Heritage. Kluwer Academic/Plenum Publishers, New York.

  • Lee HS, Kim SH, Han KS (2019) Diagnosis and Evaluation of Conservation State of Mural Paintings in Payathonzu Temple on Bagan Heritage Site in Myanmar. J Conserv Sci The Korean Society of Conservation Science for Cultural Heritage 35(5):494–507. https://doi.org/10.12654/jcs.2019.35.5.10

    Article  Google Scholar 

  • Lu S et al (2010) Ecophysiology of Fe-cycling bacteria in acidic sediments. Appl Environ Microbiol 76:8174–8183

    Article  CAS  Google Scholar 

  • Ma Y, Zhang H, Du Y et al (2015) The community distribution of bacteria and fungi on ancient wall paintings of the Mogao Grottoes. Sci Rep 5:7752. https://doi.org/10.1038/srep07752

    Article  CAS  Google Scholar 

  • Matteini M (2008) Inorganic treatments for the consolidation and protection of stone artefacts. Conserv Sci Cult Herit 8(1):13–27. https://doi.org/10.6092/issn.1973-9494/1393

    Article  Google Scholar 

  • Metin S, Soslu A (2018) The Altıkapılı Cave Church at Pisidia Hüseyin. Adalya 21:311–334

    Google Scholar 

  • Morillas H, de Mendonça F, Filho F, Derluyn H et al (2020) Decay processes in buildings close to the sea induced by marine aerosol: Salt depositions inside construction materials. Sci Total Environ 721:137687. https://doi.org/10.1016/j.scitotenv.2020.137687

    Article  CAS  Google Scholar 

  • Nason G, Lithgow K (1999) Environmental monitoring of the great painted staircase at Knole. The Conservator 23:57–67. https://doi.org/10.1080/01410096.1999.9995139

    Article  Google Scholar 

  • Nugari MP, Pietrini AM, Caneva G, Imperi F, Visca P (2009) Biodeterioration of mural paintings in a rocky habitat: The Crypt of the Original Sin (Matera, Italy). Int Biodeterior Biodegradation 63(6):705–711

    Article  CAS  Google Scholar 

  • Pavlik I, Gersl M, Bartos M et al (2018) Nontuberculous mycobacteria in the environment of Hranice Abyss, the world’s deepest flooded cave (Hranice karst, Czech Republic). Environ Sci Pollut Res 25:23712–23724. https://doi.org/10.1007/s11356-018-2450-z

    Article  CAS  Google Scholar 

  • Petrushkova JP, Lyalikova NN (1986) Microbiologial degradation of lead-containing pigments in mural paintings. Stud Conserv 31:65–69

    Google Scholar 

  • Piacenza E, Presentato A, Di Salvo F et al (2020) A combined physical–chemical and microbiological approach to unveil the fabrication, provenance, and state of conservation of the Kinkarakawa-gami art. Sci Rep 10:16072. https://doi.org/10.1038/s41598-020-73226-6

    Article  CAS  Google Scholar 

  • Pietrini AM, Ricci S, Nugari MP (2008) Churches and crypts. In: Caneva G, Nugari MP, Salvadori O (eds) Plant Biology for Cultural Heritage. Biodeterioration and Conservation. Getty Conservation Institute, New York, pp 179–183

    Google Scholar 

  • Portillo MC, Gonzalez JM, Saiz-Jimenez C (2008) Metabolically active microbial communities of yellow and grey colonizations on the walls of Altamira Cave. Spain. J Appl Microbiol 104(3):681–91. https://doi.org/10.1111/j.1365-2672.2007.03594.x

    Article  CAS  Google Scholar 

  • Presentato A, Armetta F, Spinella A et al. (2020) Formulation of mesoporous silica nanoparticles for controlled release of antimicrobials for stone preventive conservation Frontiers in Chemistry 8:699. Manuscript ID: 555668https://doi.org/10.3389/fchem.2020.00699

  • Presentato A, Lampis S, Vantini A et al (2020) On the Ability of Perfluorohexane Sulfonate (PFHxS) Bioaccumulation by Two Pseudomonas sp. Strains Isolated from PFAS-Contaminated Environmental Matrices. Microorganisms 8:92

    Article  CAS  Google Scholar 

  • Pruesse E, Peplies J, Glockner FO (2012) SINA: Accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28(14):1823–1829. https://doi.org/10.1093/bioinformatics/bts252

    Article  CAS  Google Scholar 

  • Purpura G (1999) Le pitture della grotta di Santa Margherita, Kalos – L’arte in Sicilia. Kalos 6:30

    Google Scholar 

  • Randazzo L et al (2015) Flos Tectorii degradation of mortars: An example of synergistic action between soluble salts and biodeteriogens. J Cult Herit 16:838–847

    Article  Google Scholar 

  • Meilunas RJ, Bentsen JG, Steinberg A (1993) Analysis of Aged Paint Binders by FTIR Spectroscopy. Stud Conserv 35:33–51

    Google Scholar 

  • Remmas N, Melidis P, Voltsi C, Athanasiou D, Ntougias S (2017) Novel hydrolytic extremely halotolerant alkaliphiles from mature landfill leachate with key involvement in maturation process. J Environ Sci Health A Tox Hazard Subst Environ Eng 52(1):64–73. https://doi.org/10.1080/10934529.2016.1229931

    Article  CAS  Google Scholar 

  • Renda V, Alvarez de Buergo M, Saladino ML, Caponetti E (2020) Assessment of protection treatments for carbonatic stone using nanocomposite coatings”. Prog Org Coat 141:105515

    Article  CAS  Google Scholar 

  • Renda V, Mollica Nardo V, Anastasio G, Caponetti E, Vasi CS, Saladino ML, Armetta F, Trusso S, Ponterio RC (2019) A multivariate statistical approach of X-ray fluorescence characterization of a large collection of reverse glass paintings Spectrochimica Acta - Part B Atomic SpectroscopyVolume 159:105655

  • Rizzo G, Ercoli L, Parlapiano M (2008) Characterization of mortars from ancient and traditional, water supply systems in Sicily. J Therm Anal Calorim 92(1):323–330

    Article  CAS  Google Scholar 

  • Roccardi A, Ricci S, Pietrini AM (2008) Semienclosed environments. In: Caneva G, Nugari MP, Salvadori O (eds) Plant Biology for Cultural Heritage Biodeterioration and Conservation. Getty Conservation Institute, New York, pp 206–210

    Google Scholar 

  • Rodrigues JD, Ferreira Pinto AP, Nogueira R, Gomes A (2018) Consolidation of lime mortars with ethyl silicate, nanolime and barium hydroxide Effectiveness Assessment with Microdrilling data. J Cult Herit 29:43–53

    Article  Google Scholar 

  • Rossi M, Galizzi A, Mastromei G, et al (2006) Bacillus subtilis gene cluster involved in calcium carbonate biomineralization Chiara Barabesi. J Bacteriol 189(1):228–235. https://doi.org/10.1128/JB.01450-06

  • Russ J, Pohl D, M, L von Nagy C, et al (2017) Strategies for 14C Dating the Oxtotitlán Cave Paintings, Guerrero, Mexico. Adv Archaeol Pract 5:170–183

    Article  Google Scholar 

  • Salvadori B, Errico V, Mauro M, Melnik E, Dei L (2003) Evaluation of Gypsum and Calcium Oxalates in Deteriorated Mural Paintings by Quantitative FTIR Spectroscopy. Spectrosc Lett 36(5–6):501–513

    Article  CAS  Google Scholar 

  • Saunders D, Kirby J (2004) The Effect of Relative Humidity on Artists’ Pigments. National Gallery Technical Bulletin 25:62–72

    Google Scholar 

  • Scatigno C, Gaudenzi S, Sammartino MP, Visco G (2016) A microclimate study on hypogea environments of ancient roman building. Sci Total Environ 566–567:298–305

    Article  CAS  Google Scholar 

  • Schabereiter-Gurtner C, Piñar G, Vybiral D, Lubitz W, Ro ̈lleke S (2001) Rubrobacter-related bacteria associ- ated with rosy discolouration of masonry and lime wall paintings. Arch Microbiol 176(5):347–354. https://doi.org/10.1007/s002030100333

    Article  CAS  Google Scholar 

  • Schabereiter-Gurtner C, Saiz-Jimenez C, Pinar G et al (2002) Phylogenetic 16S rRNA analysis reveals the presence of complex, partly unknown bacterial communitiesin Ttito bustillo cave, Spain, on its palaeolithic paintings. Environ Microbiol 4:392–400

    Article  CAS  Google Scholar 

  • Slížková Z, Drdácký M, Viani A (2015) Consolidation of weak lime mortars by means of saturated solution of calcium hydroxide or barium hydroxide. J Cult Herit 16(4):452–460

    Article  Google Scholar 

  • Sofia S, Elini K, Anglos D, Spyros A (2014) Egg yolk identification and aging in mixed paint binding media by NMR spectroscopy. John Wiley & Sons, Ltd

  • Spyros A, Anglos D (2006) Studies of organic paint binders by NMR spectroscopy. Applied Physics A. Materials Science & Processing A 83:705–508

    Article  CAS  Google Scholar 

  • Stefanis NA, Theoulakis P, Pilinis C (2009) Dry deposition effect of marine aerosol to the building stone of the medieval city of Rhodes, Greece. Build Environ 44(2):260–270

    Article  Google Scholar 

  • Sun DL, Gao YZ, Ge XY, Shi ZL, Zhou NY (2020) Special Features of Bat Microbiota Differ From Those of Terrestrial Mammals. Front Microbiol 11:1040. https://doi.org/10.3389/fmicb.2020.01040

    Article  Google Scholar 

  • Takahashi S, Tomita J, Nishioka K, Hisada T, Nishijima M (2014) Development of a prokaryotic universal primer for simultaneous analysis of Bacteria and Archaea using next-generation sequencing. PLoS One 9(8):105592. https://doi.org/10.1371/journal.pone.0105592

    Article  CAS  Google Scholar 

  • Tascon M, Mastrangelo N, Gallegos D, Marte F (2017) Determination of materials and techniques involved in the mural paintings of San Miguel Church, Argentina. J Raman Spectrosc 48:1356–1364

    Article  CAS  Google Scholar 

  • Tomaselli L, Lamenti G, Bosco M, Tiano P (2000) Biodiversity of photosynthetic micro-organisms dwelling on stone monuments. Int Biodeterior Biodegradation 46:251–258

    Article  Google Scholar 

  • Tortora M, Sfarra S, Chiarini M et al (2016) Non-destructive and micro-invasive testing techniques for characterizing materials, structures and restoration problems in mural paintings. Appl Surf Sci 387:971–985

    Article  CAS  Google Scholar 

  • Vahur S, Teearu A, Peets P, Joosu L, Leito I (2016) ATR-FT-IR spectral collection of conservation materials in the extended region of 4000–80 cm–1. Anal Bioanal Chem 408:3373–3379

    Article  CAS  Google Scholar 

  • Vettori S, Bracci S, Cantisani E et al (2016) A multi-analytical approach to investigate the state of conservation of the wall paintings of Insula 104 in Hierapolis (Turkey). Microchem J 128:279–287

    Article  CAS  Google Scholar 

  • Walujkar SA et al (2019) Utilizing the iron tolerance potential of Bacillus species for biogenic synthesis of magnetite with visible light active catalytic activity. Colloid Surf B 177:470–478

    Article  CAS  Google Scholar 

  • Yasir M (2018) Analysis of bacterial communities and characterization of antimicrobial strains from cave microbiota. Braz J Microbiol 49(2):248–257. https://doi.org/10.1016/j.bjm.2017.08.005

    Article  CAS  Google Scholar 

  • Zhang G, Gong C, Gu J, Katayama Y, Someya T, Gu JD (2019) Biochemical reactions and mechanisms involved in the biodeterioration of stone world cultural heritage under the tropical climate conditions International. Biodeterior Biodegradation 143:10472

    Google Scholar 

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Acknowledgements

We would like to express our gratitude to Soprintendenza di Trapani for allowing us to investigate and collect samples from the paintings at Santa Margherita Cave and to thank Ignazio Sottile (BC Sicilia) for his fruitful cooperation.

The authors would like to express our gratitude to Comune di Catellammare del Golfo for the interest to the research. EC, GC, and FA thank Mr I. Sottile for useful discussions and guide us to reach the site. Thanks are due to Prof. G. Traina of the Academy of Art of Palermo for his precious suggestions about the methodology to use for the restoration of the paintings.

Eugenio Caponetti wants to dedicate this paper to the memory of prof. Sebastiano Tusa, who had inspired this work during a discussion about the conservation of paintings in Sicily caves.

Funding

A.P. and F.A thank MIUR for the Project PON Ricerca e Innovazione 2014–2020 – Avviso DD 407/2018 “AIM Attrazione e Mobilità Internazionale” (AIM1808223). This work is part of the project “Development and Application of Innovative Materials and processes for the diagnosis and restoration of Cultural Heritage—DELIAS”—PON03PE 00214 2 (Programma Operativo Nazionale Ricerca e Competitività2007–2013).

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FA, EC, and MLS: Conceptualization and methodology. JC and FA: XRD, XRF, and FT-IR investigations. PdS: petrographic analysis. RA, AP and LV: biological test, bioinformatic analysis and data analysis. AS: NMR data analysis. FA, EC, MS, RA, and AP: writing–review and editing. All authors contributed to the article and approved the submitted version.

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Correspondence to Francesco Armetta or Alessandro Presentato.

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Armetta, F., Cardenas, J., Caponetti, E. et al. Conservation state of two paintings in the Santa Margherita cliff cave: role of the environment and of the microbial community. Environ Sci Pollut Res 29, 29510–29523 (2022). https://doi.org/10.1007/s11356-021-17211-0

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