Abstract
Botulism is a rare, acute, life-threatening neuro-paralysis. The digestive onset may raise diagnostic issues. The objective of our study was to analyze the clinical and epidemiological data of patients diagnosed with botulism and hospitalized in “Gavril Curteanu” Municipal Clinical Hospital (Oradea, Romania). Detection of the Clostridium botulinum neurotoxin (type B) in the laboratories of the National Institute of Medical-Military Research Development “Cantacuzino”, Bucharest, Romania (using the mouse bioassays method) confirmed the diagnostics. The statistical analysis was performed using the IBM SPSS software. Forty-eight patients with the diagnosis of foodborne botulism were hospitalized between 2012 and 2018 (36.92% of the total number of cases of botulism reported in Romania). The winter-spring period was the period when most cases were registered (36 patients, p=0.020). Women from rural areas were predominant (but not statistically significant), and the patients’ mean age was 39.93±12.59 years. The most common source/cause of botulism was the consumption of homemade ham. The incubation period was 26.68±22.94 h, and 2.25±1.68 days passed from the clinical onset to diagnosis. The results prove that botulism is still a public health issue, especially in areas where homemade products are prepared using inappropriate/unsafe recipes/procedures.
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References
Anniballi F, Auricchio B, Fiore A, Lonati D, Locatelli CA, Lista F, Fillo S, Mandarino G, De Medici D (2017) Botulism in Italy, 1986 to 2015. Euro Surveill 22:30550
Anonymous (1997) World Medical Association declaration of Helsinki Recommendations guiding physicians in biomedical research involving human subjects. JAMA 277:925–926
Anonymous (2016) European Centre for Disease Prevention and Control. Annual Epidemiological Report 2016 – Botulism, Stockholm
Anonymous (2018) European Centre for Disease Prevention and Control. Surveillance systems overview for 2018, Stockholm
Apel PJ, Alton T, Northam C, Ma J, Callahan M, Sonntag WE, Li Z (2009) How age impairs the response of the neuromuscular junction to nerve transection and repair: an experimental study in rats. J Orthop Res 27:385–393
Codrean A, Dumitrascu DL, Codrean V, Tit DM, Bungau S, Aleya S, Rus M, Fratila O, Nistor-Cseppento DC, Aleya L, Negrut N (2020) Epidemiology of human giardiasis in Romania: a 14 years survey. Sci Total Environ 705:135784
Krzowska-Firych J, Mikłaszewska A, Tomasiewicz K (2020) Foodborne botulism in eastern Poland: a hospital-based retrospective study and epidemiological data review. Int J Food Prop 23:609–615
Lindström M, Korkeala H (2006) Laboratory diagnostics of botulism. Clin Microbiol Rev 19:298–314
Lonati D, Schicchi A, Crevani M, Buscaglia E, Scaravaggi G, Maida F, Cirronis M, Petrolini VM, Locatelli CA (2020) Foodborne botulism: clinical diagnosis and medical treatment. Toxins (Basel) 12
Neghina AM, Marincu I, Moldovan R, Iacobiciu I, Neghina R (2010) Foodborne botulism in southwest Romania during the post-communism period 1990–2007. Int J Infect Dis 14:e96–e101
Negruţ N, Khan S, Bungau S, Zaha D, Aron R, Bratu O, Diaconu C, Ionita Radu F (2020) Diagnostic challenges in gastrointestinal infections. Rom J Mil Med CXXIII:83–90
Negrut N, Bungau S, Behl T, Khan SA, Vesa CM, Bustea C, Nistor-Cseppento DC, Rus M, Pavel FM, Tit DM (2020a) Risk factors associated with recurrent Clostridioides difficile infection. Healthcare (Basel) 8:352
Negrut N, Nistor-Cseppento DC, Khan SA, Pantis C, Maghiar TA, Maghiar O, Aleya S, Rus M, Tit DM, Aleya L, Rahdar A, Bungau S (2020b) Clostridium difficile infection epidemiology over a period of 8 years—a single centre study. Sustainability 12:4439
Nishiura H (2007) Incubation period as a clinical predictor of botulism: analysis of previous izushi-borne outbreaks in Hokkaido, Japan, from 1951 to 1965. Epidemiol Infect 135:126–130
Pauwels L, Chalavi S, Swinnen SP (2018) Aging and brain plasticity. Aging (Albany NY) 10:1789–1790
Rao AK, Sobel J, Chatham-Stephens K, Luquez C (2021) Clinical guidelines for diagnosis and treatment of botulism, 2021. MMWR Recomm Rep 70:1–30
Rasetti-Escargueil C, Lemichez E, Popoff MR (2020) Human botulism in France, 1875–2016. Toxins (Basel) 12:338
Scalfaro C, Auricchio B, De Medici D, Anniballi F (2019) Foodborne botulism: an evolving public health challenge. Infect Dis (Lond) 51:97–101
Sobel J, Dill T, Kirkpatrick CL, Riek L, Luedtke P, Damrow TA (2009) Clinical recovery and circulating botulinum toxin type F in adult patient. Emerg Infect Dis 15:969–971
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All authors contributed equally to this paper. NN, LA, TB, CCD, MAM, EEB, MMT, and SB: conceptualization, methodology, writing—original draft, writing—review & editing; NN and SB: software, visualization; LA and TB: formal analysis, investigation; NN, CCD, SB: data curation, supervision, draft reviewing; LA and SB: supervision, resources, project coordination. All authors read and approved the final manuscript.
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Negrut, ., Aleya, L., Behl, T. et al. Epidemiology of botulism in the north-western Romania—a 7-year survey. Environ Sci Pollut Res 28, 64234–64240 (2021). https://doi.org/10.1007/s11356-021-15576-w
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DOI: https://doi.org/10.1007/s11356-021-15576-w