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Chlorogenic acid inhibits apoptosis in thiram-induced tibial dyschondroplasia via intrinsic pathway

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Abstract

Tibial dyschondroplasia (TD) is a common skeletal disease occurred in growth plate of fast-growing broilers. Thiram is a sort of chemical used for pesticide and fungicide. The excessive use of thiram increased the threat to animal and human health. In this study, we aimed to investigate the therapeutic mechanism of chlorogenic acid (CGA) on thiram-induced tibial dyschondroplasia. Broiler chickens were divided into three different groups, e.g., control, TD, and CGA. CGA was administrated after the induction of TD from 4th day to 7th day. Biochemical analysis was performed to detect the content of calcium (Ca) and phosphorus (P). Histological changes and degradation of extracellular matrix were observed through hematoxylin-eosin (H & E) and Masson staining. To further determine the mechanism, TUNEL staining and western blot were also performed to detect the apoptosis changes in growth plate of all groups. The results showed the disproportionation of Ca and P content and upregulation of apoptosis during the development of TD. But, after the administration of CGA, the ratio of Ca:P was upregulated, and the apoptosis was also downregulated. The current study shows the toxic effect of thiram on chickens and suggests that CGA is associated with a mechanism that plays a significant role in apoptosis induced by thiram in poultry industry.

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Acknowledgements

We thank Lihong Zhang, Shucheng Huang, and Xiaole Tong for helpful discussion about experiment design and analysis.

Funding

This work was financially supported by the National Natural Science Foundation of China [grant number No. 31873031], the National Key Research and Development Program of China [grant number No. 2017YFD0502200], and Tibet Agricultural and Animal Husbandry University flexible introduction of talent project funding.

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Author notes

  1. Jialu Zhang and Bihao Luo contributed equally to this work.

Authors and Affiliations

  1. College of Animal Husbandry and Veterinary Medicine, Tibet Agricultural and Animal Husbandry University, Nyingchi, 860000, Tibet, People’s Republic of China

    Jialu Zhang & Jiakui Li

  2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China

    Jialu Zhang, Juanjuan Liu, Muhammad Fakhar-e-Alam Kulyar & Jiakui Li

  3. College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Bihao Luo & Kangkang Guo

  4. Faculty of Veterinary and Animal Sciences, University of Poonch, Rawalakot, Azad Jammu and Kashmir, 12350, Pakistan

    Muhammad Waqas

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  1. Jialu Zhang
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Contributions

Jialu Zhang: conceptualization, formal analysis. Jialu Zhang, Kangkang Guo, and Jiakui Li: writing, review and editing. Jialu Zhang and Jiakui Li: conceptualization. Jialu Zhang: investigation. Jialu Zhang, Bihao Luo, Juanjuan Liu: formal analysis. Jialu Zhang: software. Jialu Zhang: methodology. Muhammad Waqas, Muhammad Fakhar-e-Alam Kulyar: investigation.

Corresponding authors

Correspondence to Kangkang Guo or Jiakui Li.

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The study was approved by the Ethical Committee of the Huazhong Agricultural University (Permit No. 4200695757).

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Responsible Editor: Mohamed M. Abdel-Daim

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Highlights

• Evaluate thiram toxic effect on bone metabolism parameters.

• The application of chlorogenic acid recovered the disorders of Ca and P levels and matrix degradation in chickens which suffered tibial dyschondroplasia.

• Chlorogenic acid can alleviate the tibial dyschondroplasia through inhibiting the intrinsic apoptosis pathway.

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Zhang, ., Luo, B., Liu, J. et al. Chlorogenic acid inhibits apoptosis in thiram-induced tibial dyschondroplasia via intrinsic pathway. Environ Sci Pollut Res 28, 68288–68299 (2021). https://doi.org/10.1007/s11356-021-15286-3

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  • DOI: https://doi.org/10.1007/s11356-021-15286-3

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