Abstract
Interest in phytochemical therapy methods in the treatment of diabetes is increasing day by day. Although the antidiabetic and antioxidant effects of Cistus laurifolius L. (CL) have been mentioned, the systemic effects remain unknown. The present study aims at evaluating the antidiabetic effects of the CL aqueous extract via metformin on streptozotocin (STZ)-induced diabetic rats. Forty male Wistar albino rats were divided into five groups of eight animals each: control, diabetic group (55mg/kg STZ), STZ+125mg/kg CL, STZ+250mg/kg CL, and STZ+100mg/kg metformin. The effects of CL and metformin on oxidative, apoptotic, and inflammatory pathways were comparatively investigated. In addition, nuclear factor-κB (NFκB), tumor necrosis factor-alpha (TNF-α), and interleukin (IL)-1β expressions analysis were carried out. CL treatment resulted in a significant improvement in blood glucose levels, lipid profile, pancreatic markers, and liver and kidney function tests. A 250mg/kg CL treatment decreased by 67.9%, 31.6%, 66.8%, 28.3%, and 31.4% in the total oxidant capacity, NFκB, TNF-α, IL-1β, caspase3, and cytochrome c levels, respectively, compared to the diabetic group. Additionally, CL treatments showed a dose-dependent reduction in NFκB, TNF-α, and IL-1β expression levels. A 250mg/kg CL treatment exhibited a greater increase (by 9.6%) in total antioxidant capacity than metformin. CL treatment provided histologically more improvement in the brain, heart, pancreas, spleen, liver, kidney, and testicular tissues compared to the metformin group. Our results suggest that the single treatment of CL aqueous extract at the low doses may have stronger short-term anti-diabetic effects than metformin. Therefore, further studies are needed regarding the long-term hypoglycemic effect or treatment of CL aqueous extract.
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The experimental procedure on animals was confirmed by the Eskisehir Osmangazi University Animal Experiments Local Ethics Committee (Approval number: 678-1).
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CH, FK, and GK made experimental design and participated in data collection. CH, FK, and HS formed the experimental diabetes model in rats. CH, FK, and GK analyzed and interpreted biochemical measurements. EY provided the plant material and prepared its extract. YK analyzed the total flavonoid and phenolic compounds in the plant extract. DBD performed the histological examination of the brain, heart, pancreas, spleen, liver, kidney, and testis. CH was a major contributor in writing the manuscript. All authors read and approved the final manuscript.
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Hacioglu, C., Kar, F., Kara, Y. et al. Comparative effects of metformin and Cistus laurifolius L. extract in streptozotocin-induced diabetic rat model: oxidative, inflammatory, apoptotic, and histopathological analyzes. Environ Sci Pollut Res 28, 57888–57901 (2021). https://doi.org/10.1007/s11356-021-14780-y
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DOI: https://doi.org/10.1007/s11356-021-14780-y