Abstract
Cadmium (Cd) is a common pollutant in the aquatic environment, which puts the health and safety of aquatic organisms and humans at risk. In the present study, the freshwater crab Sinopotamon henanense was exposed to Cd (0, 50, 100, and 500 μg·L-1) for 14 d (0–14th d), followed by 21 d (14–35th d) of depuration. The changes in Cd bioaccumulation, microstructure, biomacromolecules (polysaccharides, neutral lipids, DNA and total proteins), and biochemical parameters (SOD, CAT, GR, TrxR, MDA and AChE) in the gills and hepatopancreas were tested. The injured microstructure, activated antioxidant system, increased MDA, and inhibited AChE of the gills and hepatopancreas responded with progressive bioaccumulation of Cd. Meanwhile, the polysaccharides and neutral lipids in the hepatopancreas reduced and DNA synthesis enhanced. During depuration, more than 58.80 ± 8.53% and 13.84 ± 12.11% of Cd was excreted from the gills and hepatopancreas, respectively. Recovery of microstructure and biomacromolecules as well as alleviated oxidative damage and neurotoxicity were also found in these two organs. Additionally, based on PCA, Ihis, GR and MDA were identified as the optimal biomarkers indicating the health status of crabs. In conclusion, S. henanense could resist Cd stress through antioxidant defence and self-detoxification.
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The data and materials used or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We want to allocate thanks to Dr. Xuelei Hu and Prof. Hans-Uwe Dahms for guidance in our research.
Funding
This study was funded by the National Natural Science Foundation of China (Grant No. 31672293), Shanxi Scholarship the Council of China (No. 2016-1), and the Shanxi Key Research and Development Program of China (No. 201703D221008-3).
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L J, W E M and Z C Y performed sample collection of crabs. X Z H was a major operator of the experiment and contributor in writing the manuscript. All authors modified and approved the final manuscript.
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Highlights
• Polysaccharides and neutral lipids of hepatocytes were susceptible to Cd stress.
• S. henanense resisted Cd through the antioxidant system during 21 d of depuration.
• The gills had a higher EC, better recovery capability than hepatopancreas.
• Ihis, GR and MDA were suitable biomarkers showing the health status of S. henanense.
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Xu, Z., Liu, J., Wang, E. et al. Detoxification and recovery after cadmium exposure in the freshwater crab Sinopotamon henanense. Environ Sci Pollut Res 28, 58050–58067 (2021). https://doi.org/10.1007/s11356-021-14528-8
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DOI: https://doi.org/10.1007/s11356-021-14528-8