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Protective role of yeast beta-glucan on lead acetate-induced hepatic and reproductive toxicity in rats

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Abstract

Lead (Pb) is one of the most common environmental pollutants and causes adverse effects on human and animal health. This study aimed to evaluate the protective role of beta-glucan against hepatic and reproductive toxicity induced by lead acetate. A total of 28 Sprague Dawley male rats were distributed into four groups (n = 7). The control group was intraperitoneally injected saline (1 ml/kg b.w.) daily for 21 days, the Pb group was intraperitoneally injected lead acetate (15 mg/kg b.w.) daily for 21 days, the beta-glucan group was orally administrated beta-glucan (50 mg/kg b.w.) daily for 21 days, and the Pb + beta-glucan group was intraperitoneally injected lead acetate (15 mg/kg b.w.) daily following the oral administration of beta-glucan (50 mg/kg b.w.) daily for 21 days. Results showed that feed intake in the Pb + beta-glucan group was significantly increased in comparison with that of the Pb group (p < 0.001). We also found that liver malondialdehyde (MDA) level was increased significantly in the Pb group (p < 0.01), while glutathione (GSH) level (p < 0.05), glutathione peroxidase (GSH-Px) (p < 0.05), and catalase (CAT) (p < 0.01) activities were reduced when they were compared with control. Moreover, Pb administration increased expression of pro-apoptotic protein Bax, the ratio of Bax/Bcl-2, and decreased the expression of the antiapoptotic protein Bcl-2 (p < 0.01). Also, Pb was found to cause a significant decrease in sperm motility (p < 0.01) and sperm concentration (p < 0.05) but increase in sperm tails and total sperm anomalies (p < 0.05). These findings were partially preserved by the administration of beta-glucan. Taken together, these results indicated that beta-glucan has the potential to alleviate the Pb-induced toxicity.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work supported by the Fırat University Research Fund of Elazig, as Master thesis study Project (Project number: VF.18.13).

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Authors and Affiliations

  1. Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Firat University, 23119, Elazig, Turkey

    Pinar Tatli Seven & Seda Iflazoglu Mutlu

  2. Department of Plant and Animal Production, Vocational School of Sivrice, Firat University, Elazig, Turkey

    Ismail Seven

  3. Department of Physiology, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey

    Gozde Arkali

  4. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey

    Seyma Ozer Kaya

  5. Breeding Sheep and Goat Association, Elazig, Turkey

    Osman Ersin Kanmaz

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  1. Pinar Tatli Seven
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  2. Seda Iflazoglu Mutlu
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  3. Ismail Seven
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  4. Gozde Arkali
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Contributions

PTS designed the study and wrote first draft of the manuscript. PTS, IS, SIM, OEK and GA conducted the experiments. GA performed oxidative stress and protein expression analyses. SOK performed sperm analyses. SIM, SOK, and GA analyzed the data and prepared figures. All authors read and approved the final manuscript.

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Correspondence to Seda Iflazoglu Mutlu.

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Ethics approval and consent to participate

This study was conducted with the ethics committee approval of Fırat University Animals Experiments Local Ethics Committee dated 11.07.2018, with a decision no. 131 and protocol no 2018/67.

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The authors declare no conflict of interest.

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Responsible Editor: Mohamed M. Abdel-Daim

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Tatli Seven, P., Iflazoglu Mutlu, S., Seven, I. et al. Protective role of yeast beta-glucan on lead acetate-induced hepatic and reproductive toxicity in rats. Environ Sci Pollut Res 28, 53668–53678 (2021). https://doi.org/10.1007/s11356-021-14398-0

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