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Shifts in enzymatic activities and microbial community structures in the bioenhanced treatment of ship domestic sewage under microaerobic conditions

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Abstract

A bioenhancement strategy for improving the anaerobic degradation efficiency of ship domestic sewage under microaerobic conditions was proposed in this study. Strains Stenotrophomonas sp. MSPP05 and Prevotella sp. MSPP07 with high organic-degrading efficiency and extracellular hydrolase yield were used for the bioenhancement of activated sludge. In batch experiments, the removal rates of chemical oxygen demand and total nitrogen reached 94.5% and 66.9% after 72 h of degradation. The activities of dehydrogenase, extracellular amylase, and protease in the treatment group were 1.2, 1.4, and 2.0 times higher than those in the control group. Microbial community analysis showed that exogenous enhanced strains competed with original microorganisms and became dominant. One-stage continuous stirred tank reactor with bioenhanced activated sludge ran steadily for 90 days with average effluent COD and TN concentrations of 87.5 and 14.6 mg/L. The feasibility of improving organic-degrading efficiency through bioenhancement by using exogenous hydrolase-producing strains was confirmed under microaerobic conditions. This work provided a theoretical basis for improving treatment effects and developing a new technique for ship domestic sewage treatment.

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Availability of data and material

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Funding

This work was supported by the China MSA project (grant number 80817006), which is gratefully acknowledged.

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Authors and Affiliations

  1. Marine Engineering College, Dalian Maritime University, Dalian, 116026, People’s Republic of China

    Qing Chen, Wanqing Wu & Fang Wei

  2. Environmental Science and Engineering College, Dalian Maritime University, Dalian, 116026, People’s Republic of China

    Linghua Zhang

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  1. Qing Chen
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  2. Wanqing Wu
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  3. Linghua Zhang
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  4. Fang Wei
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Contributions

Qing Chen integrated the data and was a major contributor in writing the manuscript. Wanqing Wu and Linghua Zhang provided the research idea and instruments. Fang Wei performed the determination of chemical indexes. All authors read and approved the final manuscript.

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Correspondence to Wanqing Wu.

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Chen, Q., Wu, W., Zhang, L. et al. Shifts in enzymatic activities and microbial community structures in the bioenhanced treatment of ship domestic sewage under microaerobic conditions. Environ Sci Pollut Res 28, 51242–51250 (2021). https://doi.org/10.1007/s11356-021-14232-7

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  • DOI: https://doi.org/10.1007/s11356-021-14232-7

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