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Alteration in antioxidant status in slow and fast alleles of EPHX1 gene polymorphisms among wood workers

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Abstract

Occupational wood dust exposure may be associated with various health effects, especially in wood industry. These effects may be due to inducing oxidative stress which is related to inflammations. Biochemical assessment of antioxidant enzyme activities illustrated role of oxidative stress (OS) on its depletion. Super oxide dismutase, glutathione peroxidase (GPx) and catalase (CAT) were analyzed in 50 exposed workers and 50 control subjects. Also, macrophage inflammatory protein-2 was assessed among these workers as it was produced upon dust exposure. Microsomal epoxide hydrolase (EPHX1) enzyme shared in the protective mechanism against wood dust oxidative stress. It plays a dual role in the metabolism of environmental pollutants, detoxification, and bioactivation. Gene polymorphisms of EPHX1 may be associated with variations in enzyme activity. Polymorphisms in exons 3 and 4 have resulted in either decreased (slow conjugating allele) or increased (fast conjugating allele) activity in vitro. We aimed to evaluate the associations between EPHX1 polymorphisms and change in antioxidant status (SOD, CAT, and GPx) among wood dust exposed workers. EPHX1 genotyping in exon 3 and exon 4 polymorphisms was carried out by PCR-RFLP. Our result shows a significant reduction in enzymatic antioxidants (SOD, CAT, and GPx) levels with significant rise in MIP-2 levels in worker group. Also, there are significant variations in SOD, CAT, and GPx levels as well as in MIP-2 in different genotypes of EPHX polymorphisms in exon 3 or 4 (specially in Hist-Hist genotypes in both exons). We can conclude an alteration in antioxidant status in both slow and fast allele of EPHX gene polymorphisms with release of MIP-2 protein in wood workers.

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Data sharing not applicable to this article as no datasets were generated or analyzed during the current study.

References

  • Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126

    CAS  Google Scholar 

  • Antognelli C, Gambelunghe A, Talesa VN, Muzi G (2014) Reactive oxygen species induce apoptosis in bronchial epithelial BEAS-2B cells by inhibiting the antiglycation glyoxalase I defence: involvement of superoxide anion, hydrogen peroxide and NF-κB. Apoptosis. 19(1):102–116

    Article  CAS  Google Scholar 

  • Bislimovska D, Petrovska S, Minov J (2015) Respiratory symptoms and lung function in never-smoking male workers exposed to hardwood dust. Open Access Maced J Med Sci. 500–505

  • Bruschweiler ED, Wild P, Huynh CK, Savova-Bianchi D, Danuser B, Hopf NB (2016) DNA damage among wood workers assessed with the comet assay. Environ Health Insights 10:105–112

    Article  Google Scholar 

  • Bukvic N, Lovreglio P, Fanelli M, Susca FC, Ballini A, Lastella P, Foà V, Fustinoni S, Soleo L, Guanti G (2009) Influence of some detoxification enzyme polymorphisms on cytogenetic biomarkers between individuals exposed to very low doses of 1, 3-butadiene. Journal of occupational and environmental medicine; 811-821

  • Coleshowers CL, Oguntibeju OO, Ukpong M, Truter EJ (2010) Effects of Methotrexate on Antioxidant Enzyme Status in a Rodent Model. Medical Technology SA 24(1):4–9

    Google Scholar 

  • Driscoll KE 2000 (TNFalpha and MIP-2: role in particle-induced inflammation and regulation by oxidative stress); Toxicol Lett., 177-183

  • Driscoll KE, Hassenbein DG, Carter J, Poynter J, Asquith TN, Grant RA, Whitten J, Purdon MP, Takigiku R (1993) Macrophage inflammatory proteins 1 and 2: expression by rat alveolar macrophages, fibroblasts, and epithelial cells and in rat lung after mineral dust exposure. Am J Respir Cell Mol Biol 8(3):311–318

    Article  CAS  Google Scholar 

  • Dubovskaya LV, Rybina TM, Bakakina YS, Kardash OF, Denisevich NP, Volotovski ID (2015) Genetic predisposition to chronic dust bronchitis among potash miners. Journal of Medical and Biological Science Research 1(6):55–61

    Google Scholar 

  • Erkisi Z, Yaylim-Eraltan I, Turna A, Görmüs U, Camlica H, Isbir T (2010) Polymorphisms in the microsomal epoxide hydrolase gene: role in lung cancer susceptibility and prognosis. Tumori 96(5):756–763

    Article  CAS  Google Scholar 

  • Gaballah IF, Helal SF, Mourad BH (2018) Early detection of lung cancer potential among Egyptian wood workers. Int J Occup Environ Health 23(2):120–127

    Article  Google Scholar 

  • Handayani N, Sohilauw DSS, Tolinggi S, Keman S, KetutSudiana I (2014) Effect of Endotoxin Levels Lipopolysaccharide (LPs) In wood dust against increased levels of C-reactive protein and decreasing the lung function sawmill workers in factory Mojoagung jombang. International Refereed Journal of Engineering and Science (IRJES) 3(8):01–05

    Google Scholar 

  • Hassett C, Robinson KB, Beck NB, Omiecinski CJ (1994) The human microsomal epoxide hydrolase gene (EPHX1): complete nucleotide sequence and structural characterization). Genomics,; 433–442

  • Kiyohara C, Yoshimasu K, Takayama K, Nakanishi Y (2006) EPHX1 polymorphisms and the risk of lung cancer: a HuGE review. Epidemiology. 17(1):89–99

    Article  Google Scholar 

  • Kurai J, Watanabe M, Tomita K, Sano H, Yamasaki A, Shimizu E (2014) Influence of Asian dust particles on immune adjuvant effects and airway inflammation in asthma model mice. PLoS One 9(11):1–7

    Article  Google Scholar 

  • Li H, Fu WP, Hong ZH (2012) Microsomal epoxide hydrolase gene polymorphisms and risk of chronic obstructive pulmonary disease: A comprehensive meta-analysis. Oncol Lett:1022–1030

  • Magagnotti N, Nannicini C, Sciarra G, Spinelli R, Volpi D (2013) Determining the Exposure of Chipper Operators to Inhalable Wood Dust. The Annals of Occupational Hygiene:784–792

  • Mard I, Sakly M, Amara S (2017) Aluminum oxide nanoparticles induced cognitive deficits and oxidative stress in frontal cortex and cerebellum of rat. Advanced Journal of Toxicology: Current Research 1(1):7–14

    Google Scholar 

  • Nishikimi M, Appaji N, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 46(2):849–854

    Article  CAS  Google Scholar 

  • Očkajová A, Kučerka M, Kminiak R, Krišťák Ľ, Igaz R, Réh R (2020) Occupational exposure to dust produced when milling thermally modified wood. Int J Environ Res Public Health 17(5):1478

    Article  Google Scholar 

  • Plaglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    Google Scholar 

  • Qin CC, Liu YN, Hu Y, Yang Y, Chen Z (2017) Macrophage inflammatory protein-2 as mediator of inflammation in acute liver injury. World J Gastroenterol 23:3043–3052

    Article  CAS  Google Scholar 

  • Rekhadevi PV, Mahboob M, Rahman MF, Grover P (2009) Genetic damage in wood dust-exposed workers. Mutagenesis. 24(1):59–65

    Article  CAS  Google Scholar 

  • Singh SR, Hijam D, Dubey A, Devi NO, Jamir S, Longkumer C, Nandinibala L, Singh TP, Singh WG (2015) Study of oxidative stress status in type 2 diabetic patients. International Journal of Contemporary Medical Research 2(1):20–27

    CAS  Google Scholar 

  • Suliman YAO, Ali D, Alarifi S, Harrath AH, Mansour L, Alwasel SH (2015) Evaluation of cytotoxic, oxidative stress, proinflammatory and genotoxic effect of silver nanoparticles in human lung epithelial cells). Environmental toxicology,; 149-160

  • Tan X, Wang YY, Chen XY, Xian L, Guo JJ, Liang GB, Chen MW (2014) Quantitative assessment of the effects of the EPHX1 Tyr113His polymorphism on lung and breast cancer. Genetics and molecular research,; 7437-7446

  • Tumer TB, Sahin G, Arinç E (2012) Association between polymorphisms of EPHX1 and XRCC1 genes and the risk of childhood acute lymphoblastic leukemia. Arch Toxicol 86:431–439

    Article  CAS  Google Scholar 

  • Václavíková R, Hughes DJ, Souček P (2015) Microsomal epoxide hydrolase 1 (EPHX1): Gene, structure, function, and role in human disease.Gene. 1-8

  • Wippich C, Rissler J, Koppisch D, Breuer D (2020) estimating respirable dust exposure from inhalable dust exposure. Ann Work Expo Health 64(4):430–444

    Article  Google Scholar 

  • Wultsch G, Nersesyan A, Kundi M, Wagner KH, Ferk F, Jakse R, Knasmueller S (2015) Impact of exposure to wood dust on genotoxicity and cytotoxicity in exfoliated buccal and nasal cells. Mutagenesis 30(5):701–709

    Article  CAS  Google Scholar 

  • Xu X, Hua H, Fan B, Sun Q, Guo X, Zhang J (2015) EPHX1 rs2234922 polymorphism and lung cancer susceptibility in Asian populations: a meta-analysis. Journal of thoracic disease:1125–1129

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Acknowledgment

The authors express their sincere thanks to Prof Dr Amal Saad, Prof. of Environmental & Preventive Medicine, National Research Centre, for her encouragement during the study and her help in analyzing the data.

Funding

The study was funded by the National Research Centre. The grant number is (11205).

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Authors and Affiliations

  1. Department of Environmental and Occupational Medicine, Environmental Research Division, National Research Centre, Dokki, Giza, Egypt

    Mona M. Taha, Eman M. Shahy & Heba Mahdy-abdallah

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  1. Mona M. Taha
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  2. Eman M. Shahy
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  3. Heba Mahdy-abdallah
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Contributions

MMT designed the study and performed genetic analysis, EMS conducted the biochemical study. HMA interviewed the participants and performed clinical examination. MMT analyzed and interpreted the data and wrote the manuscript. All authors have critically reviewed and approved the final draft and are responsible for the content and similarity index of the manuscript.

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Correspondence to Mona M. Taha.

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Consents were obtained from all subjects before onset of work. Approval of the Ethical committee of the National Research Centre was taken prior to the study (Registration number 11075).

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The paper has not been published before and is not considered for publication elsewhere. The corresponding Author took the consent of all Co-authors before sending the paper for publication.

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The authors declared no potential conflicts of interest.

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Taha, M.M., Shahy, E.M. & Mahdy-abdallah, H. Alteration in antioxidant status in slow and fast alleles of EPHX1 gene polymorphisms among wood workers. Environ Sci Pollut Res 28, 49678–49684 (2021). https://doi.org/10.1007/s11356-021-14166-0

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  • DOI: https://doi.org/10.1007/s11356-021-14166-0

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