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Preventive effect of Bacillus mojavensis levan against carbon tetrachloride and cisplatin toxicity: in vivo study

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Abstract

This study is the first to investigate the hepato- and nephron-preventive effect of levan from Bacillus mojavensis (BM-levan) against toxicity induced by carbon tetrachloride (CCl4) and cisplatin. Thirty-six male albino rats weighing between 230 and 250 g were used for this experiment. The groups received multiples doses of BM-levan and were compared to the untreated group. The in vitro and in vivo biological potentials of BM-levan were evaluated by measuring its antioxidant capacity as well as its hepato- and nephron-protective activities in rat models. The investigations highlighted a significant in vitro antioxidant activity indicated by the radical-scavenging capacity, the reducing power, and the total antioxidant activity measurement. In addition, results demonstrate that BM-levan supplementation during 8 weeks (100 mg/kg body weight) significantly (p < 0.05) decreased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities and remarkably (p < 0.05) attenuated the altered lipid profile by decreasing the levels of triglycerides (TG) and total cholesterol (TC), LDL cholesterol (LDL-C) and by enhancing the HDL cholesterol (HDL-C) content, when compared with the CCl4 group. BM-levan also reduced the content of plasma renal biomarkers (urea, creatinine, and uric acid) in the cisplatin-treated group. Moreover, BM-levan inhibited hepatic and renal oxidative stress generated by CCl4 and cisplatin administration, through the enhancement of the antioxidant catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and the diminishment of lipid peroxidation. The harmful effects of CCl4 or cisplatin on hepatic and renal histology were found to be decreased by the addition of BM-levan. Therefore, BM-levan has proved promising for biomedical applications thanks to its in vitro and in vivo antioxidant properties.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

CCl4 :

Carbon tetrachloride

AST:

Aspartate aminotransferase

ALT:

Alanine aminotransferase

LDL-C:

LDL cholesterol

HDL-C:

HDL cholesterol

TG:

Triglycerides

TC:

Total cholesterol

GPx:

Glutathione peroxidase

SOD:

Superoxide dismutase

CAT:

Catalase

MDA:

Malondialdehyde

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Acknowledgements

This work was funded by the Ministry of Higher Education and Scientific Research, Tunisia. The authors would like to express their sincere gratitude to Mr. Haythem Chiboub, Professeur agrégé of English Language, from the Faculty of Sciences of Gafsa (Tunisia) who provided professional support in the proofreading, editing, and language polishing of the present manuscript.

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Authors and Affiliations

  1. Laboratory of Plants Improvement and Valorization of Agroressources, National School of Engineering of Sfax (ENIS), University of Sfax, 3038, Sfax, Tunisia

    Anissa Haddar, Mariem Hamed, Assaad Sila & Semia Ellouz-Chaabouni

  2. Department of Life Sciences, Faculty of Sciences of Gafsa, Gafsa University, 2112, Gafsa, Tunisia

    Anouar Feriani & Assaad Sila

  3. Research Unit of Macromolecular Biochemistry and Genetic, Faculty of Sciences of Gafsa, University of Gafsa, 2112, Gafsa, Tunisia

    Anouar Feriani

  4. Common Service Unit of Bioreactor coupled with an ultrafilter, National School of Engineering, Sfax University, Sfax, Tunisia

    Semia Ellouz-Chaabouni

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  1. Anissa Haddar
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  2. Anouar Feriani
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  3. Mariem Hamed
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  4. Assaad Sila
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  5. Semia Ellouz-Chaabouni
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Contributions

Anissa Haddar: investigation, conceptualization, methodology, data curation, writing – original draft.

Anour Feriani: investigation, methodology, formal analysis.

Mariem Hamed: methodology, data curation, formal analysis.

Assaad Sila: methodology, investigation, resources, data curation.

Semia Ellouz-Chaabouni: supervision, resources, visualization, writing – review and editing.

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Correspondence to Anissa Haddar.

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Haddar, A., Feriani, A., Hamed, M. et al. Preventive effect of Bacillus mojavensis levan against carbon tetrachloride and cisplatin toxicity: in vivo study. Environ Sci Pollut Res 28, 50117–50126 (2021). https://doi.org/10.1007/s11356-021-14147-3

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