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The protective effects of hesperidin and curcumin on 5-fluorouracil–induced nephrotoxicity in mice

Environmental Science and Pollution Research volume 28, pages 47046–47055 (2021)Cite this article

Abstract

Nephrotoxicity is a very important complication of 5-fluorouracil (5-FU)–treated cancer patients. Increased oxidative stress, kidney damage, and apoptosis play an important role in the pathogenesis of nephrotoxicity caused by 5-FU. In this study, protective effects of two natural compounds, hesperidin and curcumin, on experimentally induced kidney damage in mice with 5-FU were determined. Application of 5-FU resulted in severe histopathological changes and severe renal failure with increased serum urea and creatinine levels. Also, 5-FU–induced kidney damage, increased levels of malondialdehyde (MDA), decreased superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activity, and glutathione (GSH) level have been demonstrated. Also, where 5-FU is in the concentration of caspase-3 and 8-OHdG immune-positive cells and therefore causes apoptosis and DNA damage in kidney tissue cells. However, especially high doses of hesperidin and curcumin treatment significantly improved 5-FU–induced oxidative stress/lipid peroxidation, apoptosis/DNA damage, and renal dysfunction. Based on these data, our results suggest that hesperidin and curcumin may be used as new and promising agents against 5-FU–induced nephrotoxicity.

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Affiliations

  1. Department of Physiology, Veterinary Faculty, Kafkas University, Kars, Turkey

    Volkan Gelen

  2. Department of Physiology, Veterinary Faculty, Ataturk University, Erzurum, Turkey

    Emin Şengül & Samet Tekin

  3. Department of Pathology, Veterinary Faculty, Ataturk University, Erzurum, Turkey

    Serkan Yıldırım

  4. School of Health Services, Agri Ibrahim Cecen University, Agri, Turkey

    Esra Senturk

  5. Department of Biochemistry, Veterinary Faculty, Kafkas University, Kars, Turkey

    Abdulsamed Kükürt

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  1. Volkan Gelen
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  2. Emin Şengül
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  3. Serkan Yıldırım
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  4. Esra Senturk
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  5. Samet Tekin
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  6. Abdulsamed Kükürt
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Contributions

VG, EŞ, ST, AK: experiment design, experiment application, samples collection. VG, EŞ, ES: serum markers and tissue antioxidant estimation, data curation and analysis, final reviewing. SY: histopathological and immunohistochemical investigation. All the authors contributed to the writing and editing, and they read and approved the final manuscript.

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Correspondence to Volkan Gelen.

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The study was designed and conducted according to ethical norms approved by the Atatürk University Rectorate Animal Experiments Local Ethics Committee (Erzurum, Turkey) (Protocol No. 2019/16).

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Gelen, V., Şengül, E., Yıldırım, S. et al. The protective effects of hesperidin and curcumin on 5-fluorouracil–induced nephrotoxicity in mice. Environ Sci Pollut Res 28, 47046–47055 (2021). https://doi.org/10.1007/s11356-021-13969-5

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  • DOI: https://doi.org/10.1007/s11356-021-13969-5

Keywords

  • Nephrotoxicity
  • Apoptosis
  • Curcumin
  • Hesperidin
  • Histopathology
  • Immunohistochemistry
  • Mice