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Arsenate and arsenite-induced inhibition and recovery in two diazotrophic cyanobacteria Nostoc muscorum and Anabaena sp.: study on time-dependent toxicity regulation

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Abstract

Exposure time, metal bio-accumulation, and upregulation of ascorbate-glutathione (AsA-GSH) cycle are the key factor that provide tolerance against heavy metal stress. Thus, the current study is an endeavor to prove our hypothesis that regulation of arsenate (AsV: 50, 100, and 150 mM) and arsenite (AsIII: 50, 100, and 150 μM) toxicity is time dependent (48–96 h) due to modulation in bio-accumulation pattern, AsA-GSH cycle, and non-enzymatic antioxidants in two paddy field cyanobacteria Nostoc muscorum ATCC27893 and Anabaena sp. PCC7120. After 48 h, reduction in growth associated with increased sensitivity index, As bio-accumulation, and oxidative stress was observed which further intensified after 96 h but the degree of damage was lesser than 48 h. It denotes a significant recovery in growth after 96 h which is correlated with decreased As bio-accumulation and oxidative stress due to increased efficiency of AsA-GSH cycle and non-enzymatic antioxidants. Both the species of As caused significant rise in oxidative biomarkers as evident by in -vitro analysis of O2·−, H2O2, and MDA equivalent contents despite appreciable rise in the activity antioxidative enzymes APX, DHAR, and GR. The study concludes that among both forms of arsenic, AsIII induced more toxic effect on growth by over-accumulating the ROS as evident by weak induction of AsA-GSH cycle to overcome the stress as compared to AsV. Further, with increasing the time exposure, apparent recovery was noticed with the lower doses of AsV, i.e., 50 and 100 mM and AsIII, i.e., 50 and 100 μM; however, the toxicity further aggravated with higher dose of both AsV and AsIII. Study proposes the deleterious impact of AsV and AsIII on cyanobacteria N. muscorum and Anabaena sp. but the toxicity was overcome by time-dependent recovery.

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Acknowledgements

Anuradha Patel is thankful to NFO as ‘SRF’ with award letter number (NFO-2015-17-OBC-UTT-41056) and Sanjesh Tiwari is thankful to CSIR-UGC New Delhi as ‘SRF’ with award letter number 2121430412, EU-V, 29-06-15. Authors are thankful to the Head, Department of Botany for providing necessary lab facilities.

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    1. Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Prayagraj, 211002, India

      Anuradha Patel, Sanjesh Tiwari & Sheo Mohan Prasad

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    1. Anuradha Patel
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    2. Sanjesh Tiwari
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    3. Sheo Mohan Prasad
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    SMP designed the experiment, AP and ST planned and performed the experiment, AP and ST analyzed the data, and SMP, AP, and ST wrote the manuscript.

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    Correspondence to Sheo Mohan Prasad.

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    Anuradha Patel and Sanjesh Tiwari contributed equally.

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    Patel, A., Tiwari, S. & Prasad, S.M. Arsenate and arsenite-induced inhibition and recovery in two diazotrophic cyanobacteria Nostoc muscorum and Anabaena sp.: study on time-dependent toxicity regulation. Environ Sci Pollut Res 28, 51088–51104 (2021). https://doi.org/10.1007/s11356-021-13800-1

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