Abstract
Vicia villosa Roth is a legume species with a growing application in Argentina as a cover crop (CC), a practice that favors the sustainable development of agricultural systems. However, several areas where the use of this CC provides numerous advantages are affected by high concentrations of arsenic (As). Thus, in the present work we studied hairy vetch ability to cope with arsenate [As(V)], arsenite [As(III)], and the mixture of both along with oxidative stress indexes [chlorophyll content, malondialdehyde (MDA) equivalents] as well as anatomical and histological changes in the root structure. The results obtained suggested a different behavior of hairy vetch depending on its growth stage and on metal(oid) concentration. The roots treated with the contaminant showed less turgidity, thickening of the epidermal and subepidermal parenchymal outer layers, and the presence of dark deposits. The morpho-anatomic parameters (cortex length, vascular cylinder diameter, total diameter, and vascular cylinder area) were altered in plants treated with As(V) and As(V)/As(III) whereas the roots of plants treated with As(III) did not show significant differences respect to the control. Moreover V. villosa could tolerate and remove As from soil, thus the use of this legume species seems an attractive approach to remediate As while protecting contaminated soils.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Ahmed M, Wardle D (1994) Allelopathic potential of vegetative and flowering ragwort (Senecio jacobea L.) plants against associated pasture species. Plant Soil 64:61–68. https://doi.org/10.1007/BF00010111
Armendariz AL, Talano MA, Travaglia CN, Reinoso H, Wevar Oller AL, Agostini E (2016) Arsenic toxicity in soybean seedlings and their attenuation mechanisms. Plant Physiol Biochem 98:119–127. https://doi.org/10.1016/j.plaphy.2015.11.021
Barrena R, Casals E, Colón J, Font X, Sánchez A, Puntes V (2009) Evaluation of the ecotoxicity of nanoparticles. Chemosphere 75:850–857. https://doi.org/10.1016/j.chemosphere.2009.01.078
Buscaroli A (2017) An overview of indexes to evaluate terrestrial plants for phytoremediation purposes. Ecol Indic 82:367–380. https://doi.org/10.1016/j.ecolind.2017.07.003
D’Ambrogio de Argüeso (1986) Manual de Técnicas en Histología Vegetal. Hemisferio Sur. Buenos Aires, Argentina
Dere S, Günes T, Sivaci R (1997) Spectrophotometric determination of chlorophyll A, B and total carotenoid contents of some algae species using different solvents. Botany 22:13–17
Garcia-Huidobro J, Monteith JL, Squire GR (1982) Time, temperature and germination of pearl millet (Pennisetum typhoides S. & H.): III. Inhibition of germination by short exposure to high temperature. J Exp Bot 33:288–296. https://doi.org/10.1093/jxb/36.2.338
Gerhardt KE, Gerwing PD, Greenberg BM (2017) Opinion: taking phytoremediation from proven technology to accepted practice. Plant Sci 256:170–185. https://doi.org/10.1016/j.plantsci.2016.11.016
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
Ibañez SG, Medina MI, Agostini E (2020) Vicia: a green bridge to clean up polluted environments. Appl Microbiol Biotechnol 104:13–21. https://doi.org/10.1007/s00253-019-10222-5
Ibañez SG, Merini LJ, Barros GG, Medina MI, Agostini E (2014) Vicia sativa-rhizospheric bacteria interactions to improve phenol remediation. Int J Environ Sci Technol 11:1679–1690. https://doi.org/10.1007/s13762-013-0357-2
Ibañez SG, Sosa Alderete LG, Medina MI, Agostini E (2012) Phytoremediation of phenol using Vicia sativa L. plants and its antioxidative response. Environ Sci Pollut Res 19:1555–1562. https://doi.org/10.1007/s11356-011-0664-4
Ibañez SG, Wevar Oller AL, Paisio CE, Sosa Alderete LG, González PS, Medina MI, Agostini E (2018) The challenges of remediating metals using phytotechnologies. In: Donatti E (ed) Heavy metals in the environment: microorganisms and bioremediation. CRC Press, Taylor & Francis, pp 173–195
Irshad S, Xie Z, Wang J, Nawaz A, Luo Y, Wang Y, Mehmoode S, Faheem (2020) Indigenous strain Bacillus XZM assisted phytoremediation and detoxification of arsenic in Vallisneria denseserrulata. J Hazard Mater 381:120903. https://doi.org/10.1016/j.jhazmat.2019.120903
Johansen DA (1940) Plant microtechnique, first ed. McGraw-Hill Book Co. Ltd., New York, USA
Kofroňová M, Hrdinová A, Mašková P, Soudek P, Tremlová J, Pinkas D, Lipavská H (2019) Strong antioxidant capacity of horseradish hairy root cultures under arsenic stress indicates the possible use of Armoracia rusticana plants for phytoremediation. Ecotoxicol Environ Saf 174:295–304. https://doi.org/10.1016/j.ecoenv.2019.02.028
Kumar D, Tripathi DK, Liu S, Singh VK, Sharma S, Dubey NK, Prasad SM, Kumar Chauhana D (2017) Pongamia pinnata (L.) Pierre tree seedlings offer a model species for arsenic phytoremediation. Plant Gene 11:238–246. https://doi.org/10.1016/j.plgene.2017.06.002
Kumar S, Dubey RS, Tripathi RD, Chakrabarty D, Trivedi PK (2015) Omics and biotechnology of arsenic stress and detoxification in plants: current updates and prospective. Environ Int 74:221–230. https://doi.org/10.1016/j.envint.2014.10.019
Lardone AV, Justo C, Barraco MR, Scianca CM, Miranda WR (2013) Especies de cultivos de cobertura como antecesores de maíz tardío y soja. Memoria técnica, Estación Experimental Agropecuaria General Villegas
Le Gall H, Philippe F, Domon J-M, Gillet F, Pelloux J, Rayon C (2015) Cell wall metabolism in response to abiotic stress. Plants 4:112–166. https://doi.org/10.3390/plants4010112
Li N, Wang J, Song W-Y (2016) Arsenic uptake and translocation in plants. Plant Cell Physiol 57:4–13. https://doi.org/10.1093/pcp/pcv143
Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. In: Wrolstad RE, Acree TE, An HJ et al (eds) Current protocols in food analytical chemistry. John Wiley & Sons, Inc, New York, pp F4.3.1–F4.3.8
Mohamed I, Akladious SA (2017) Changes in antioxidants potential, secondary metabolites and plant hormones induced by different fungicides treatment in cotton plants. Pestic Biochem Physiol 142:117–122. https://doi.org/10.1016/j.pestbp.2017.04.001
Nahar N, Rahman A, Nawani NN, Ghosh S, Mandal A (2017) Phytoremediation of arsenic from the contaminated soil using transgenic tobacco plants expressing ACR2 gene of Arabidopsis thaliana. J Plant Physiol 218:121–126. https://doi.org/10.1016/j.jplph.2017.08.001
Panda SK, Upadhyay RK, Nath S (2010) Arsenic stress in plants. J Agron Crop Sci 196:161–174. https://doi.org/10.1111/j.1439-037X.2009.00407.x
Pandey J, Chand S, Pandey S, Rajkumari Patra DD (2015) Palmarosa [Cymbopogon martinii (Roxb.) Wats.] as a putative crop for phytoremediation, in tannery sludge polluted soil. Ecotoxicol Environ Saf 122:296–302. https://doi.org/10.1016/j.ecoenv.2015.08.005
Patra DK, Pradhan C, Patra HK (2020) Toxic metal decontamination by phytoremediation approach: concept, challenges, opportunities and future perspectives. Environ Technol Innov 18:100672. https://doi.org/10.1016/j.eti.2020.100672
Praveen A, Mehrotra S, Singh N (2019) Mixed plantation of wheat and accumulators in arsenic contaminated plots: a novel way to reduce the uptake of arsenic in wheat and load on antioxidative defence of plant. Ecotoxicol Environ Saf 182:109462. https://doi.org/10.1016/j.ecoenv.2019.109462
Rai PK, Lee SS, Zhang M, Tsang YF, Kim K-H (2019) Heavy metals in food crops: health risks, fate, mechanisms, and management. Environ Int 125:365–385. https://doi.org/10.1016/j.envint.2019.01.067
Rai R, Pandey S, Pandey Rai S (2011) Arsenic-induced changes in morphological, physiological, and biochemical attributes and artemisinin biosynthesis in Artemisia annua, an antimalarial plant. Ecotoxicol 20:1900–1913. https://doi.org/10.1007/s10646-011-0728-8
Reginato M, Travaglia C, Reinoso H, Garello F, Luna V (2016) Salt mixtures induce anatomical modifications in the halophyte Prosopis strombulifera (Fabaceae: Mimosoideae). Flora 218:75–85. https://doi.org/10.1016/j.flora.2015.11.008
Reinoso H, Sosa L, Reginato M, Luna V (2005) Histological alterations induced by sodium sulfate in the vegetative anatomy of Prosopis strambulifera (Lam.) Benth. World J Agricultural Sci 1:109–119
Renzi JP, Chantre GR, Cantamutto MA (2014) Development of a thermal-time model for combinational dormancy release of hairy vetch (Vicia villosa ssp. villosa). Crop Pasture Sci 65:470–478. https://doi.org/10.1071/CP13430
Renzi JP, Chantre GR, Cantamutto MA (2016) Self-regeneration of hairy vetch (Vicia villosa Roth) as affected by seedling density and soil tillage method in a semi-arid agroecosystem. John Wiley & Sons Ltd. Grass Forage Sci 72:524–533. https://doi.org/10.1111/gfs.12255
Rimski-Korsakov H, Alvarez CR, Lavado RS (2015) Cover crops in the agricultural systems of the Argentine Pampas. J Soil Water Conserv 70:112–119. https://doi.org/10.2489/jswc.70.6.134A
Rodrıguez-Serrano M, Romero-Puertas CM, Pazmiño DM, Testillano PS, Risueño MC, del Río LA, Sandalio LM (2009) Cellular response of pea plants to cadmium toxicity: cross talk between reactive oxygen species, nitric oxide, and calcium. Plant Physiol 150:229–243. https://doi.org/10.1104/pp.108.131524
Ruciήska-Sobkowiak R (2016) Water relations in plants subjected to heavy metal stresses. Acta Physiol Plant 38:257–270. https://doi.org/10.1007/s11738-016-2277-5
Shah V, Daverey A (2020) Phytoremediation: a multidisciplinary approach to clean up heavy metal contaminated soil. Environ Technol Innov 18:100774. https://doi.org/10.1016/j.eti.2020.100774
Singh S, Sinha S, Saxena R, Pandey K, Bhatt K (2004) Translocation of metals and its effects in the tomato plants grown on various amendments of tannery waste: evidence for involvement of antioxidants. Chemosphere 57:91–99. https://doi.org/10.1016/j.chemosphere.2004.04.041
Singh S, Sounderajan S, Kumar K, Fulzele DP (2017) Investigation of arsenic accumulation and biochemical response of in vitro developed Vetiveria zizanoides plants. Ecotoxicol Environ Saf 145:50–56. https://doi.org/10.1016/j.ecoenv.2017.07.013
Svobodníková L, Kummerová M, Zezulka S, Babula P, Sendeckác K (2020) Root response in Pisum sativum under naproxen stress: morpho-anatomical, cytological, and biochemical traits. Chemosphere 258:127411. https://doi.org/10.1016/j.chemosphere.2020.127411
Talano MA, Cejas RB, González PS, Agostini E (2013) Arsenic effect on the model crop symbiosis Bradyrhizobium-soybean. Plant Phisiol Biochem 63:8–14. https://doi.org/10.1016/j.plaphy.2012.11.00
Tripathi P, Mishra A, Dwivedi S, Chakrabarty D, Trivedi PK, Singh RP, Tripathi RD (2012) Differential response of oxidative stress and thiol metabolism in contrasting rice genotypes for arsenic tolerance. Ecotoxicol Environ Saf 79:189–198. https://doi.org/10.1016/j.ecoenv.2011.12.019
Funding
This work was supported by the grants from Secretaría de Ciencia y Técnica-Universidad Nacional de Río Cuarto (SECyT-UNRC) (PPI 2016–2018), Fondo Nacional para la Investigación Científica y Tecnológica (FONCYT) (PICT 447/15), and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) (PIP). Sabrina G Ibañez, Claudia N Travaglia, and Elizabeth Agostini are members of the Research Career from CONICET (Argentina).
Author information
Authors and Affiliations
Contributions
Sabrina G. Ibañez did all the experiments. Claudia N. Travaglia contributed with the execution and analysis of microscopical assays. Sabrina G. Ibañez and Elizabeth Agostini designed the experiments, analyzed the results, and wrote the paper. Claudia N. Travaglia, María I. Medina, and Elizabeth Agostini critically revised and correct the manuscript.
Corresponding author
Ethics declarations
Ethics approval and consent to participate
Not applicable.
Consent for publication
Not applicable.
Competing interests
The authors declare no competing interests.
Additional information
Responsible Editor: Philipp Gariguess
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Ibañez, S.G., Travaglia, C.N., Medina, M.I. et al. Vicia villosa Roth: a cover crop to phytoremediate arsenic polluted environments. Environ Sci Pollut Res 28, 38604–38612 (2021). https://doi.org/10.1007/s11356-021-13529-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-021-13529-x