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Alleviating Cr(VI) stress in horse gram (Macrotyloma uniflorum Var. Madhu) by native Cr-tolerant nodule endophytes isolated from contaminated site of Sukinda

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Abstract

Sukinda chromite mine of Odisha is a heavily polluted site, generating huge overburden dumps. The present experiment was designed to evaluate the potential of two native nodule endophytic bacterial strains, viz. Bacillus aryabhattai AS03 (MT645244) and Rhizobium pusense AS05 (MT645243), isolated from contaminated sites to be considered remediation tool to minimize the effect of Cr toxicity on Macrotyloma uniflorum var. Madhu. The two nodule endophytic bacterial strains AS03 and AS05 exhibited tolerance to 1800 and 3000 ppm of Cr(VI) respectively in vitro when cultured alone. AAS analysis confirmed higher accumulation of Cr(VI) in roots and less accumulation in shoots which is dose-specific (bio-inoculant) either treated alone or combined. Complete absence of Cr accumulation approximately 99% in shoots of Macrotyloma was observed owing to synergistic effect of both the strains (biochar-based formulation). This study also suggests increased shoot and root length, nodule nos., and leghemoglobin content of the plant at 60 days indicating the plant growth-promoting effects of both the strains. ROS and antioxidant enzymes of the plant recorded decreasing trend in inoculated plants. However, a significant increment in transpiration rate, total photosynthetic rate, intracellular CO2 conc., and stomatal conductance in leaves was observed owing to dual inoculation. Our findings corroborate the supremacy of synergistic effect of both the strains applied in the form of biochar-based biofertilizer in enhancing growth and tolerance index of M. uniflorum cultivated in Cr(VI)-stressed soil. This investigation depicts the efficiency of the two nodule bacteria as a mixed inoculant to alleviate Cr toxicity and making the seeds safe for consumption.

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Acknowledgements

The authors acknowledge DST, New Delhi, India, for necessary funding towards upgradation of laboratory facility in Department of Botany and COE under World Bank, RUSA, Utkal University, India, to carry out the study. Furthermore, authors acknowledge Dr. Saroj Kumar Sahu and Ms. Poonam Mangaraj for their valuable contribution for preparation of map.

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  1. Shilpee Dhali and Madhusmita Pradhan contributed equally to this work.

Authors and Affiliations

  1. Department of Botany, Utkal University, VaniVihar, Bhubaneswar, Odisha, 751004, India

    Shilpee Dhali & Chinmay Pradhan

  2. Department of Microbiology, OUAT, Bhubaneswar, Odisha, 751003, India

    Madhusmita Pradhan

  3. Department of Biotechnology, Centurion University of Technology and Management, Bhubaneswar, Odisha, 752050, India

    Ranjan Kumar Sahoo

  4. Department of Soil Science and Agricultural Chemistry, OUAT, Bhubaneswar, Odisha, 751003, India

    Santanu Mohanty

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  1. Shilpee Dhali
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  2. Madhusmita Pradhan
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  4. Santanu Mohanty
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  5. Chinmay Pradhan
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Contributions

Shilpee Dhali: formal analysis, data curation, writing—original draft, investigation. Madhusmita Pradhan: formal analysis, data curation, writing—original draft and review. Ranjan Kumar Sahoo: writing—review and editing, methodology, statistical analysis. Santanu Mohanty: review and editing, data curation, conceptualization. Chinmay Pradhan: methodology, conceptualization, data curation, project administration, supervision.

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Correspondence to Chinmay Pradhan.

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Highlights

• Isolation of Cr(VI)-tolerant nodule endophytes from contaminated site of Sukinda.

• Dual bacterial inoculation resulted in no accumulation of Cr in shoots at 60 days.

• Nodule endophytes benefited the crop with respect to morpho-physicochemical characteristics.

• Plants with biochar formulation showed 94.97% tolerance index under Cr toxicity.

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Dhali, S., Pradhan, M., Sahoo, R.K. et al. Alleviating Cr(VI) stress in horse gram (Macrotyloma uniflorum Var. Madhu) by native Cr-tolerant nodule endophytes isolated from contaminated site of Sukinda. Environ Sci Pollut Res 28, 31717–31730 (2021). https://doi.org/10.1007/s11356-021-13009-2

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