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Antidiabetic and antioxidant activity of phlorotannins extracted from the brown seaweed Cystoseira compressa in streptozotocin-induced diabetic rats

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Abstract

Diabetes mellitus is considered a set of diseases that lead to high glucose level due to the absolute or relative absence of insulin. The study investigated the antioxidant activity and antidiabetic effect of phlorotannins extracted from brown seaweed Cystoseira compressa. Phlorotannins were extracted from C. compressa. It was confirmed by 2,4 dimethoxy benzaldehyde assay (DMBA), ultraviolet spectra, Fourier transform infrared spectroscopy, and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The free radical scavenging activity of phlorotannins was estimated by total antioxidant capacity, 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity, and 2,2 azino-bis3-ethylbenthiazoline-6-sulfonic acid assays. Four groups of albino rats used in this study include control normal, control phlorotannins extract, diabetic by intraperitoneally administering of streptozotocin, and diabetic treatment with 60 mg/kg of phlorotannin extract after 4 weeks of diabetes induction. The main compound identified by UPLC-MS/MS in C. compressa extract belonged to the fuhalol. C. compressa extract showed high antioxidant properties. Phlorotannins significantly decreased serum glucose, liver malondialdehyde, and α-amylase, glucosidase activities. However, total antioxidant capacity, serum insulin, hepatic glutathione, and AMPKα2 expression in skeletal muscle were improved compared to the diabetic group. The histopathological examination showed that phlorotannins markedly reduced damage in β cells of pancreases. Phlorotannins from C. compressa have efficient antioxidant activity and the antidiabetic effect that may be utilized in human health.

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Acknowledgments

The authors wish to thank Dr. Amira R. El Barky for her kind co-operation during this work. Deep thanks from authors to the editor of Environmental Science and Pollution Research Journal and reviewers who improve this manuscript to appear in this good form.

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Authors and Affiliations

  1. Phycology Division, Botany Department, Faculty of Science, Tanta University, Tanta, Egypt

    Saly Gheda

  2. Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt

    Mai Abdel Naby, Tarek Mohamed & Abeer Khamis

  3. Marine and Environmental Research Center (MARE), Department of Life Sciences, University of Coimbra, 3000-456, Coimbra, Portugal

    Leonel Pereira

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Contributions

Conceptualization, SG and TM; methodology, software, SG and MA; validation, SG, TM, and AK; formal analysis MA; investigation, MA; resources, SG and AK; data curation, TM, SG, LP, and AK; writing original draft preparation, SG, TM, and MA; writing—review and editing, TM, SG, LP, and AK; visualization, TM, SG, and AK supervision, TM, SG, LP, and AK. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Saly Gheda.

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Handlings of animals were in accordance with the National ethical guidelines for the care of the Institution of animal care and use committee (ICUC), Faculty of Science, Tanta University, project identification code: Rec-Sci-Tu-0028, and date of approval: 27-7-2017.

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Gheda, S., Naby, M.A., Mohamed, T. et al. Antidiabetic and antioxidant activity of phlorotannins extracted from the brown seaweed Cystoseira compressa in streptozotocin-induced diabetic rats. Environ Sci Pollut Res 28, 22886–22901 (2021). https://doi.org/10.1007/s11356-021-12347-5

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