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Haematological, biochemical, enzymological and histological responses of Labeo rohita exposed to methyl orange dye solution treated with Oedogonium subplagiostomum AP1

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Abstract

The present investigation is an attempt to assess the impact of untreated methyl orange and Oedogonium subplagiostomum AP1 treated methyl orange dye solutions on Labeo rohita. The behavioural response, mortality, haematological (red blood corpuscles (RBC), packed cell volume (PCV), haemoglobin (Hb), white blood corpuscles (WBC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC)), biochemical (plasma glucose and protein), enzymological (aspartate amino transaminases (AST) and alanine amino transaminases (ALT)) and histological examination (gills, liver and kidney) of Labeo rohita are exposed to untreated and treated methyl orange dye solutions were assessed on 7th day. The fish exposed to tap water and treated dye solution showed normal behavioural response whereas abnormal behaviour was noted in fish exposed to untreated dye solution. Similar trend was recorded in the mortality rate of the fishes. Fish exposed to untreated dye solution showed reduction in RBC, PCV, Hb, MCHC, plasma glucose and plasma protein, increased level of WBC, MCV and MCH and also alteration in AST and ALT thereby indicating the toxicity of the dye. No such reduction and alteration were observed in haematological, biochemical and enzymological levels of fishes exposed to tap water and treated dye solution indicating the non-toxic nature of the degraded metabolites of dye. Histological examination of fishes exposed to methyl orange dye revealed necrosis and haemorrhage in the gills and hepatocytes, congested and shrunken glomeruli in kidney thereby indicating the toxicity of the dye. The histoarchitecture of control and algae-treated fishes showed no structural changes indicating the non-toxic nature of the degraded metabolites of the dye. The results concluded that methyl orange dye solution treated with O. subplagiostomum AP1 can be explored for aquacultural purposes owing to its non-toxic nature.

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Acknowledgements

The authors wish to place their record of thanks to the authorities of Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, for the support given to conduct the study and DST CURIE (No. SR/CURIE/PHASE II/01/2014) to utilize the chemicals required for the study.

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  1. Department of Zoology, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, 641 043, Tamil Nadu, India

    Maruthanayagam Alaguprathana & Mani Poonkothai

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MP contributed to the study conception and design. MA performed experiment and data collection. The data was analysed and interpreted by MP and MA. The draft of the manuscript was written by MP. All authors read and approved the final manuscript.

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Alaguprathana, M., Poonkothai, M. Haematological, biochemical, enzymological and histological responses of Labeo rohita exposed to methyl orange dye solution treated with Oedogonium subplagiostomum AP1. Environ Sci Pollut Res 28, 17602–17612 (2021). https://doi.org/10.1007/s11356-020-12208-7

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