Abstract
The influence of grazing and mining on arbuscular mycorrhizal fungi (AMF) species in grassland were studied around the opencast coal mine pit. The two sampling transects had been designed which was located in the grazing area and in the non-grazing area in the grassland on the north side of the opencast coal mining area. Besides, two control points with the direct distance of 8Â km to the opencast coal mining area have been designed. The two points are located in the inside grassland station and outside the grassland station. We collected 3 replicate rhizosphere soil in every point which is from the two transects. We analyzed the genetic diversity of AMF, measured the chemical properties and enzyme of rhizosphere soil of Cleistogenes squarrosa, and explored their correlations using redundancy analysis. And we identified the factors affecting species diversity of AMF around the mining area. Our results showed that grazing on the grassland around the opencast coal mine will aggravate soil degradation and reduce the species richness and diversity of arbuscular mycorrhizal fungi in rhizosphere soil, which can be a theoretical basis for the scientific management of the grassland around the opencast mining area.
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Data availability
The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
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We thank the editor and reviewers for their valuable suggestions on an earlier version of the manuscript.
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The State Key Research Development Program of China (Grant No. 2016YFC0501106) funded this study.
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Yinli Bi and Nan Guo conceptualized and designed the study. Sampling, experiment and data analysis were performed by Nan Guo, Yanxu Zhang, and Kun Wang. The first draft of the manuscript was written by Nan Guo and Yinli Bi. Li Xiao and Anna Krzyszowska Waitkus commented on and revised previous versions of the manuscript. All authors read and approved the final manuscript.
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Guo, N., Bi, Y., Zhang, Y. et al. Grazing and mining influence the population of arbuscular mycorrhizal fungi around the opencast coal mine pit. Environ Sci Pollut Res 28, 21425–21436 (2021). https://doi.org/10.1007/s11356-020-11656-5
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DOI: https://doi.org/10.1007/s11356-020-11656-5