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Enhanced antioxidant capacity and upregulated transporter genes contribute to the UV-B-induced increase in blinin in Conyza blinii

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Abstract

Conyza blinii (C. blinii) is a traditional Chinese medicinal plant mainly grown in Sichuan, China. C. blinii is suitable for studying the mechanism of plant tolerance to UV-B due to its living conditions, characterized by a high altitude and exposure to strong ultraviolet radiation. Our results showed that the growth and photosynthetic activity of C. blinii were improved under a specific intensity of UV-B, rather than being significantly inhibited. Although UV-B increased the content of reactive oxygen species (ROS) in C. blinii, the activities of antioxidative enzymes were elevated, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), which contributed to the elimination of ROS. Additionally, the content of blinin, the characteristic diterpene in C. blinii, was markedly increased by UV-B. Furthermore, RNA sequencing analyses were used to explore the molecular mechanism of UV-B tolerance in C. blinii. According to the results, most of the key enzyme genes in the blinin synthesis pathway were upregulated by UV-B. In addition, 23 upregulated terpene transporter genes were identified, and these genes might participate in blinin transport during the response to UV-B. Taken together, these results implied that enhanced antioxidant capacity and upregulated transporter genes contributed to increased synthesis of blinin in response to UV-B in C. blinii.

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Acknowledgments

We thank all the colleagues in our laboratory for providing useful discussions and technical assistance. We are very grateful to the editor and reviewers for critically evaluating the manuscript and providing constructive comments for its improvement.

Funding

This research was supported by a study on improving the content of blinin in Conyza blinii H. Lév. by metabolic engineering (2018HH0074), a study on DNA Damage by Body Fluid Chloride and its Mechanism supported by Sichuan Science and Technology Program (2020YFH0136), and entrepreneurship training program for college students (201810626106). Funds were used for the design of the study and collection, analysis, and interpretation of data and in writing the manuscript, as well as in the open access payment.

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  1. Junyi Zhan and Qin Yang contributed equally to this work.

Authors and Affiliations

  1. College of Life Science, Sichuan Agricultural University, Ya’an, China

    Junyi Zhan, Qin Yang, Zhiyi Lin, Tianrun Zheng, Maojia Wang, Wenjun Sun, Tongliang Bu, Zizhong Tang, Chenglei Li, Xueyi Han, Haixia Zhao, Qi Wu, Zhi Shan & Hui Chen

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  1. Junyi Zhan
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Contributions

J.-Y.Z and Q.Y designed the research; J.-Y.Z, Q.Y, T.-R.Z, Z.-Y.L, W.-J.S, M.-J.W, and T.W performed the research; J.-Y.Z, T.-L.B, and Z.-Z.T analyzed the data; J.-Y.Z, Q.Y, and Z.-Y.L wrote the paper. All authors read and approved the final manuscript.

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Correspondence to Hui Chen.

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Zhan, J., Yang, Q., Lin, Z. et al. Enhanced antioxidant capacity and upregulated transporter genes contribute to the UV-B-induced increase in blinin in Conyza blinii. Environ Sci Pollut Res 28, 13275–13287 (2021). https://doi.org/10.1007/s11356-020-11502-8

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