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Evaluation of direct and biochar carrier-based inoculation of Bacillus sp. on As- and Pb-contaminated technosol: effect on metal(loid) availability, Salix viminalis growth, and soil microbial diversity/activity

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Abstract

Phytomanagement manipulates the soil-plant system to lower the risk posed by contaminated soils. In this process, the addition of amendments, such as biochar, and bacteria can improve the fertility of poor contaminated soils and consequently ameliorate plant growth. A number of studies based on the inoculation of soil with microorganisms of the genus Bacillus, previously isolated from contaminated sites, revealed positive effects on soil properties and plant growth. Furthermore, when the Bacillus isolates were used in association with biochar, better results were obtained, as biochar can ameliorate soil properties and serve as habitat for microorganisms. Accordingly, a mesocosm study was set-up using a mining technosol amended with biochar and inoculated with an endogenous Bacillus isolate, to evaluate the effect of inoculation on soil properties, metal(loid) immobilization, and Salix viminalis growth. Two inoculation methods were compared: (1) direct inoculation of bacteria (Bacillus sp.) and (2) inoculation using biochar as a carrier. Results showed that the Bacillus isolate modified soil properties and ameliorated plant growth, while having a reduced effect on metal(loid) accumulation. The microbial activity was also stimulated, and the community composition was shifted, more importantly when biochar was used as a carrier. In conclusion, this research revealed an improvement of the plant growth and microbial activity after the addition of the endogenous bacterium to the analyzed former mining soil, with better results recorded when a carrier was used.

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Acknowledgments

The authors wish to thank Dr Lucie Zinger for her help in the analysis of the NGS data.

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Authors and Affiliations

  1. University of Orléans, INRA USC1328, LBLGC EA1207, Orleans, France

    Manhattan Lebrun, Florie Miard, Romain Nandillon, Domenico Morabito & Sylvain Bourgerie

  2. Department of Biosciences and Territory, University of Molise, Pesche, IS, Italy

    Manhattan Lebrun, Antonio Bucci, Dalila Trupiano, Gino Naclerio & Gabriella S. Scippa

  3. French Geological Survey (BRGM), Orleans, France

    Romain Nandillon

  4. IDDEA, Environmental consulting engineering, Olivet, France

    Romain Nandillon

  5. ISTO, UMR 7327, CNRS/Orleans University, Orleans, France

    Romain Nandillon

Authors
  1. Manhattan Lebrun
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  2. Florie Miard
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  3. Antonio Bucci
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  4. Dalila Trupiano
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  5. Romain Nandillon
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  6. Gino Naclerio
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  7. Gabriella S. Scippa
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  8. Domenico Morabito
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  9. Sylvain Bourgerie
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Contributions

Manhattan Lebrun, Antonio Bucci, Dalila Trupiano, Romain Nandillon, Gino Naclerio, Gabriella Scippa, Domenico Morabito, and Sylvain Bourgerie contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Manhattan Lebrun and Florie Miard. The first draft of the manuscript was written by Manhattan Lebrun and Antonio Bucci, Dalila Trupiano, Romain Nandillon, Gino Naclerio, Gabriella Scippa, Domenico Morabito, and Sylvain Bourgerie commented on previous versions of the manuscript. All authors (Manhattan Lebrun, Florie Miard, Antonio Bucci, Dalila Trupiano, Romain Nandillon, Gino Naclerio, Gabriella Scippa, Domenico Morabito and Sylvain Bourgerie) read and approved the final manuscript.

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Correspondence to Sylvain Bourgerie.

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Lebrun, M., Miard, F., Bucci, A. et al. Evaluation of direct and biochar carrier-based inoculation of Bacillus sp. on As- and Pb-contaminated technosol: effect on metal(loid) availability, Salix viminalis growth, and soil microbial diversity/activity. Environ Sci Pollut Res 28, 11195–11204 (2021). https://doi.org/10.1007/s11356-020-11355-1

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